Detection of Differences in mRNA Expression Regulated by Salt-Stress in Mangrove Cultured Cells.

Yamada, Akiyo; Saitoh, Takeo; Mimura, Tetsuro; Ozeki, Yoshihiro

doi:10.5511/plantbiotechnology.19.145

Cited by 12 publications

(6 citation statements)

References 12 publications

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“…The asterisk indicates a statistically significant difference from 0% at Po0.05 using Dunnett's test. exposure of up to 48 h in most studies (Gilbert et al, 2002;Yamada et al, 2002;Jithesh et al, 2006;Ezawa and Tada, 2009). Two types of adaptation mechanisms are possible to cope with the salinity: short-term and long-term.…”

Section: Discussionmentioning

confidence: 99%

“…Numerous genome-wide screening studies have been devoted to identify the salt tolerance genes in halophytic or glycophytic plant (Zhu, 2000;Ueda et al, 2002;Yamada et al, 2002;Miyama and Hanagata, 2007;Ezawa and Tada, 2009). Nevertheless, terpenoid synthase gene has not been identified as salt tolerance gene.…”

Section: Discussionmentioning

confidence: 99%

“…A number of studies have shown that salinity stress increased the expression of a number of genes and level of proteins (Sugihara et al, 2000;Ueda et al, 2002;Yamada et al, 2002). Our previous study also showed that the salt stress induced a change in the concentration of terpenoids in mangrove roots and leaves (Oku et al, 2003).…”

Section: Introductionmentioning

confidence: 88%

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Expression of terpenoid synthase mRNA and terpenoid content in salt stressed mangrove

Basyuni

Baba

Inafuku

et al. 2009

Journal of Plant Physiology

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Expression of terpenoid synthase mRNA and terpenoid content in salt stressed mangrove

Basyuni

Baba

Inafuku

et al. 2009

Journal of Plant Physiology

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“…Transcriptome assembly was accomplished based on left.fq and right.fq using Trinity (r20140413p1) (MG et al, 2011). Gene function was annotated based on the following databases: Nr (NCBI nonredundant protein sequences) (v0.2.28) (Nguyen et al, 2007); Nt (NCBI non-redundant nucleotide sequences) (v2.2.28+) (Nguyen et al, 2007); Pfam (Protein family) (HMMER 3) (Finn et al, 2008); KOG/COG (Clusters of Orthologous Groups of proteins) (v0.2.28); Swiss-Prot (A manually annotated and reviewed protein sequence database) (v0.2.28); KO (KEGG Ortholog database) (r140224) (Kanehisa et al, 2008); and GO (Gene Ontology) (b2g4pipe_v2.5) (Götz et al, 2008). The gene expression levels were estimated by RSEM (v1.2.15) (Bo and Dewey, 2011) for each sample.…”

Section: Rna-seq Analysismentioning

confidence: 99%

Transcriptome and Flavonoids Metabolomic Analysis Identifies Regulatory Networks and Hub Genes in Black and White Fruits of Lycium ruthenicum Murray

Fan

Qin

et al. 2020

Front. Plant Sci.

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Lycium ruthenicum Murry. is a highly nutritional cash crop due to its fruit abundant anthocyanins. To understand the complex metabolic networks underlying the color formation in black and white fruits of L. ruthenicum, we conducted transcriptome and flavonoid metabolic profiling to identify the candidate genes possibly involved in flavonoid biosynthesis. As a result, 147 flavonoids were identified and there was almost no anthocyanin in white fruits, while luteolin, kaempferol, and quercetin derivatives showed markedly higher abundance. Furthermore, applying weighted gene co-expression network analyses, 3 MYB, 2 bHLH, 1WRKY and 1 NAC transcription factor, associated with anthocyanin biosynthesis were identified. A bHLH transcription factor, LrAN1b showed the greatest correlations with anthocyanin accumulation with no expression in white fruits. In addition, gene function analysis and qRT-PCR experiments identified a new activated anthocyanin MYB transcription factor designed as LrAN2-like. Yeast two-hybrid and transient tobacco overexpression experiments showed that LrAN1b could interact with LrAN2-like and LrAN11 to form MBW complex to activate the anthocyanin pathway. The yeast one-hybrid experiment indicated that LrAN2-like bonded anthocyanin structural gene LrDFR and LrANS promoters. Heterologous expression of LrAN1b in tobacco can significantly increase the anthocyanin content of tobacco florals and capsules, and activate anthocyanin synthesis related genes. Taken together, an anthocyanin regulatory network model in L. ruthenicum fruit was proposed firstly and we speculate that the white fruit phenotype was due to abnormal expression of LrAN1b. The findings provide new insight into the underlying mechanism of flavonoids, laying the foundation for future functional and molecular biological research in L. ruthenicum.

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“…In the recent years, the isolation of salinity tolerant genes from a few mangrove species through the generation and analyses of expressed sequence tags (ESTs) from Avicennia marina (Mehta et al 2004) and Acanthus ebracteatus (Nguyen et al 2006), differential gene expression studies of Bruguiera spp. by using differential display (Banzai et al 2002a), cDNA Representational Difference Analyis (cDNA RDA) (Yamada et al 2002a), and suppression subtractive hybridization (SSH) (Wong et al 2007), have been reported. Besides, molecular cloning and characterization of individual genes such as superoxide dismutase (Takemura et al 2002); catalase (Takemura et al 2002); oxygen evolving enhancer protein (Sugihara et al 2000); BURP domain-containing protein (Banzai et al 2002b); cytosolic chaperonin-containing TCP-1a (Yamada et al 2002c); mangrin (Yamada et al 2002b); Na + /H + antiporter (Tanaka et al 2000) and betaine/proline transporters (Waditee et al 2002), have also shed light on the understanding of salinity tolerance of mangrove plants.…”

Section: Introductionmentioning

confidence: 99%

Functional screening for salinity tolerant genes from Acanthus ebracteatus Vahl using Escherichia coli as a host

Nguyen

Harikrishna

et al. 2007

Trees

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Salinity reduces plant growth and crop production globally. The discovery of genes in salinity tolerant plants will provide the basis for effective genetic engineering strategies, leading to greater stress tolerance in economically important crops. In this study, we have identified and isolated 107 salinity tolerant candidate genes from a mangrove plant, Acanthus ebracteatus Vahl by using bacterial functional assay. Sequence analysis of these putative salinity tolerant cDNA candidates revealed that 65% of them have not been reported to be stress related and may have great potential for the elucidation of unique salinity tolerant mechanisms in mangrove. Among the genes identified were also genes that had previously been linked to stress response including salinity tolerance, verifying the reliability of this method in isolating salinity tolerant genes by using E. coli as a host.

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Detection of Differences in mRNA Expression Regulated by Salt-Stress in Mangrove Cultured Cells.

Cited by 12 publications

References 12 publications

Expression of terpenoid synthase mRNA and terpenoid content in salt stressed mangrove

Expression of terpenoid synthase mRNA and terpenoid content in salt stressed mangrove

Transcriptome and Flavonoids Metabolomic Analysis Identifies Regulatory Networks and Hub Genes in Black and White Fruits of Lycium ruthenicum Murray

Functional screening for salinity tolerant genes from Acanthus ebracteatus Vahl using Escherichia coli as a host

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