Detection of Desmosterol in the Internal Lipids of Wool Fibers

Gale, Douglas J.; Logan, Raymond I.; Rivett, Donald E.

doi:10.1177/004051758705700909

Cited by 15 publications

(5 citation statements)

References 14 publications

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“…Even though the chloroform/methanol azeotrope (az.) (79:21) is the most widely used procedure for extracting IWL (1,12,15,16), other solvents or solvent az. such as acetone, methanol, acetone/methanol az.…”

Section: Resultsmentioning

confidence: 99%

“…In an earlier study, surface lipids and contaminants were removed from the raw, unprocessed Australian Merino wool following a sequential extraction procedure with t-butanol (5) and heptane (2.5 h) and rinsing with water (12). In the following studies, all wool samples were industrially cleaned with sodium carbonate and a nonionic polyoxyethylene surfactant to remove lanolin before being mechanically shaken and rinsed with water to eliminate vegetable matter and dust.…”

Section: Methodsmentioning

confidence: 99%

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Extraction and analysis of ceramides from internal wool lipids

Coderch

Fonollosa

Martı́

et al. 2002

J Americ Oil Chem Soc

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This study sought to obtain internal wool lipid extracts rich in ceramides from different wool types. Extraction methods, i.e., Soxhlet extraction with different organic solvents and supercritical fluid extraction with CO 2 using several polarity modifiers such as MeOH or EtOH, were optimized. The internal wool lipid content varied from 0.2 to 1.9% (based on wool weight) with a ceramide content ranging from 15 to 30% (based on extract weight). The Spanish and Russian Merino wool extracts were the richest in ceramide compounds. TLC-FID was used to quantify the different internal wool lipid extracts. A new experimental protocol that enabled us to identify most of the different ceramide types is presented. These internal wool lipid extracts, especially the ones with a high ceramide content, may be regarded as an alternative source of animal ceramides, which could be of value in the cosmetic and dermopharmaceutical industries.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Extraction and analysis of ceramides from internal wool lipids

Coderch

Fonollosa

Martı́

et al. 2002

J Americ Oil Chem Soc

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“…Although the presence of free desmosterol on wool has been demonstrated (15,34), there is only one previous study that refers to the presence of different sterols [cholesterol, hydroxy-desmosterol, and 19-norcholesta-1,3,5( 10)-triene-6one] in the wool polar lipid fraction (31). Our analyses show not only the presence of cholesterol sulfate but also the presence of different polar compounds that are sterol derivatives; in spots 6 and 7, mainly desmosterol derivatives are found, and in spots 10 and 11, mainly cholesterol but also desmosterol and hydroxycholesterol derivatives are detected.…”

Section: Resultsmentioning

confidence: 99%

Chromatographic characterization of internal polar lipids from wool

Coderch

Maza

Soriano

et al. 1995

J Americ Oil Chem Soc

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Wool internal polar lipids were isolated and separated into different fractions based on polarity. Qualitative and quantitative analyses of the different fractions were performed by thin-layer chromatography and thin-layer chromatography coupled to flame-ionization detection, respectively. Cholesterol esters, free fatty acids, sterols, ceramides, glycosylceramides, and cholesterol sulfate were the main components, with ceramides being in the highest proportion. The fatty acid composition of ceramides and glycosylceramides was determined by gas chromatography/mass spectrometry. As for other keratinized tissues, long-chain fatty acids predominated in comparison to either free fatty acids or phospholipid-linked fatty acids; in both cases, stearic and lignoceric acids were the most abundant fatty acids, and a low amount of 18-methyleicosanoic acid was found. This work opens new avenues in the study of lipid rearrangement in more complex and realistic vesicle structures than conventional liposomes. JAOCS 72, 71 5-720 (1995).

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“…The internal lipids were Soxhlet extracted from cleaned wool (12 g) with chloroform/methanol azeotrope (360 mL, 79:21 v/v) for 5 h. The lipid extracts were concentrated down to 10 mL under a stream of dry nitrogen and stored in 2/1 chloroform/methanol at 6 °C. Aliquots were dried and weighed, and the lipid percentage extractions were quantified. , Quantitative analysis was performed using thin-layer chromatography (TLC) coupled to an automated ionization detection (FID) system (Iatroscan MK-5, IATRON LAB. INC. Tokyo, Japan) with a sample spotter SES 3202/IS-01 (SES GmbH, Nieder-Olm, Germany) using an optimized analytical procedure …”

Section: Methodsmentioning

confidence: 99%

Thermodynamic and Structural Aspects of Internal Wool Lipids

et al. 2000

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Thermotropic physicochemical techniques such as differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), and electron paramagnetic resonance (EPR) were applied to liposomes made up of internal wool lipids. These vesicles can be regarded as a model of wool membranes. Differences in fluidity obtained from EPR allow us to deduce a high rigidity of the polar external section of the vesicle in contrast to the high fluidity of the hydrophobic internal section, which accounts for the thermotropic properties of the liposome. The coincidence of the thermotropic changes obtained with the EPR, FTIR, and DSC techniques allows us to infer a reversible phase transition temperature in the range of 30-40 °C, indicating a transition from a mixture of hexagonal gel-liquid crystal state to a single liquid crystal state.

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Detection of Desmosterol in the Internal Lipids of Wool Fibers

Cited by 15 publications

References 14 publications

Extraction and analysis of ceramides from internal wool lipids

Extraction and analysis of ceramides from internal wool lipids

Chromatographic characterization of internal polar lipids from wool

Thermodynamic and Structural Aspects of Internal Wool Lipids

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