Chromatographic characterization of internal polar lipids from wool

Coderch, L.; Maza, Alfons de la; Soriano, C.; Erra, P.; Parra, J. L.

doi:10.1007/bf02635661

Cited by 41 publications

(41 citation statements)

References 26 publications

(25 reference statements)

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“…Even though the chloroform/methanol azeotrope (az.) (79:21) is the most widely used procedure for extracting IWL (1,12,15,16), other solvents or solvent az. such as acetone, methanol, acetone/methanol az.…”

Section: Resultsmentioning

confidence: 99%

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Extraction and analysis of ceramides from internal wool lipids

Coderch

Fonollosa

Martı́

et al. 2002

J Americ Oil Chem Soc

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This study sought to obtain internal wool lipid extracts rich in ceramides from different wool types. Extraction methods, i.e., Soxhlet extraction with different organic solvents and supercritical fluid extraction with CO 2 using several polarity modifiers such as MeOH or EtOH, were optimized. The internal wool lipid content varied from 0.2 to 1.9% (based on wool weight) with a ceramide content ranging from 15 to 30% (based on extract weight). The Spanish and Russian Merino wool extracts were the richest in ceramide compounds. TLC-FID was used to quantify the different internal wool lipid extracts. A new experimental protocol that enabled us to identify most of the different ceramide types is presented. These internal wool lipid extracts, especially the ones with a high ceramide content, may be regarded as an alternative source of animal ceramides, which could be of value in the cosmetic and dermopharmaceutical industries.

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Section: Resultsmentioning

confidence: 99%

“…Internal wool lipids (IWL) are rich in cholesterol, FFA, cholesteryl sulfate, and ceramides and resemble those from membranes of other keratinic tissues such as human hair or the stratum corneum (SC) of skin (1,2).…”

mentioning

confidence: 99%

Extraction and analysis of ceramides from internal wool lipids

Coderch

Fonollosa

Martı́

et al. 2002

J Americ Oil Chem Soc

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“…IWL liposomes were prepared from those extracted from raw industrially scoured Merino wool provided by Corcoy SA (Terrassa, Spain). The extraction procedure was performed using the methodology described in an earlier work [19]. Both kinds of liposomes were made up as follows.…”

Section: Liposome Preparationmentioning

confidence: 99%

“…The internal lipids of wool fibre were extracted and analysed in an earlier work [19]. This lipid extract is mainly composed of ceramides (46%), free fatty acids (24%), sterols (21%) and cholesteryl sulphate (9%).…”

Section: Introductionmentioning

confidence: 99%

Effect of Internal Wool Lipid Liposomes on Skin Repair

Pera

Coderch²,

Fonollosa³

et al. 2000

Skin Pharmacol Physiol

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Intercellular lipids of the stratum corneum (SC) play a crucial role in keeping an optimal skin barrier function and in regulating the water-holding capacity. Recently, the internal lipids have been extracted from wool fibre. This lipid extract has a composition similar to that of the SC lipids. Two parameters were used to test the effect of topically applied internal wool lipids (IWL), structured as liposomes, on the water barrier functions of disturbed and intact skin: transepidermal water loss and skin capacitance. Liposomes made up of lipids that simulate the composition of the SC were also applied for comparison. The single application of the IWL liposomes on disturbed skin resulted in an accelerated recovery of water barrier functions. Daily application of these liposomes on intact skin for 5 days reinforced the skin barrier and increased the water-holding capacity. The repairing effect of the IWL enhances their suitability in the treatment, prevention and care of skin.

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“…Despite the considerable advances in the characterisation of the lipid composition of the cell membrane complex [2][3][4], little progress has been made in improving our understanding * Corresponding author. Tel.…”

Section: Introductionmentioning

confidence: 99%