Detection of Deoxyribonuclease I Along the Secretory Pathway in Paneth Cells of Human Small Intestine

Shimada, Osamu; Ishikawa, Harunori; Tosaka-Shimada, Hisami; Yasuda, Toshihiro; Kishi, Koichiro; Suzuki, Shosuke

doi:10.1177/002215549804600706

Cited by 29 publications

(21 citation statements)

References 37 publications

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“…Recently, it has been suggested that the peripheral halo mainly contains O-linked oligosaccharides with N-acetyl-galactosamine (GalNAc) and N-acetyl-d-glucosamine (GlcNAc) residues and N-linked oligosaccharides with GlcNAc residues (33). The peripheral Pc halo also contains the antimicrobial DNase I that is specifically associated with the exocrine secretory granules (34). The characteristic peripheral intermediately dense halo ( Figure 6E, left panel, arrow) characteristic of WT animals was completely absent in the Pc granules of germ-free Cd1d -/-mice and instead an "empty" halo remained ( Figure 6E, right panel, arrow).…”

Section: Figurementioning

confidence: 99%

Cd1d-dependent regulation of bacterial colonization in the intestine of mice

Nieuwenhuis¹,

Matsumoto²,

Lindenbergh³

et al. 2009

J. Clin. Invest.

148

139

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The accumulation of certain species of bacteria in the intestine is involved in both tissue homeostasis and immune-mediated pathologies. The host mechanisms involved in controlling intestinal colonization with commensal bacteria are poorly understood. We observed that under specific pathogen-free or germ-free conditions, intragastric administration of Pseudomonas aeruginosa, E. coli, Staphylococcus aureus, or Lactobacillus gasseri resulted in increased colonization of the small intestine and bacterial translocation in mice lacking Cd1d, an MHC class I-like molecule, compared with WT mice. In contrast, activation of Cd1d-restricted T cells (NKT cells) with α-galactosylceramide caused diminished intestinal colonization with the same bacterial strains. We also found prominent differences in the composition of intestinal microbiota, including increased adherent bacteria, in Cd1d -/-mice in comparison to WT mice under specific pathogen-free conditions. Germ-free Cd1d -/-mice exhibited a defect in Paneth cell granule ultrastructure and ability to degranulate after bacterial colonization. In vitro, NKT cells were shown to induce the release of lysozyme from intestinal crypts. Together, these data support a role for Cd1d in regulating intestinal colonization through mechanisms that include the control of Paneth cell function.

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Section: Figurementioning

confidence: 99%

Cd1d-dependent regulation of bacterial colonization in the intestine of mice

Nieuwenhuis¹,

Matsumoto²,

Lindenbergh³

et al. 2009

J. Clin. Invest.

148

139

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“…During chronic pancreatitis, after pancreatic duct ligation in hamsters and in diabetic mice, PCs become hyperplastic [128 -131]. Considering that pancreatic lipase-related protein 2 [121], pancreatic carboxylesterase [125], phospholipase B/lipase [132], and DNAse I [133] have been localized in PCs, PC secretions may have digestive functions in small intestinal regions distant from the pancreas, where digestive enzymes originating from the pancreas may have undergone autodegradation and degradation through microbial products. However, several other digestive enzymes including chymotrypsin, amylase, and lipase are reportedly not expressed in the small intestine [134].…”

Section: Relationship Of Pcs To Pancreatic Cellsmentioning

confidence: 99%

The multifaceted Paneth cell

Porter

Bevins

Ghosh

et al. 2002

Cellular and Molecular Life Sciences (CMLS)

361

277

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Paneth cells (PCs) were described over a century ago as granulated cells located at the base of small intestinal crypts, the 'crypts of Lieberkühn.' Various histochemical staining procedures were developed that identified PCs based on their distinctive granule-staining pattern. Early on, PCs were proposed to perform a specialized function other than absorption of digested nutrients, the predominant task of the small intestinal epithelium. Since then, many constituents of the PC granules have been biochemically characterized. The presence of various granule-associated antimicrobial substances and their release upon microbial challenge suggest that PCs function as specialized defense cells in the small intestine. Altered resistance to microbial infection in animal models with disrupted or augmented PC function provides further support for the host defense role of PCs. Other PC components suggest that PCs may also participate in the regulation of lumenal ionic composition, crypt development, digestion, and intestinal inflammation.

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“…The function of DNases within the small intestine is debated and very few researchers purified DNases from small intestine. DNase I was purified and studied from human small intestine in 1998 [13], while the present work purified a DNase II from the lysosomes of jejunum of the small intestine in human. The authors used two methods for the purification of the enzyme: a classical method that used affinity chromatography and the immunoabsorption technique [14].…”

Section: Introductionmentioning

confidence: 99%

A Novel Method For Purifying a DNase From Lysosomal Fraction From Human Small Intestine

Shikara¹,

Al-jaff²

2012

Turk J Bioch

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Aims:The purification of an acid DNase related DNase II family from lysosomes of human small intestine (jejunum). Materials and Methods: Two different methods: a column chromatography series, including phosphocellulose, CM-cellulose and Sephadex G-200 and a novel procedure that use immunoabsorbent technique were used in the purification. Results:The purified DNase is an endonuclease that consisted of one polypeptide chain (monomer) with a molecular mass of 28-32kDa with an optimal pH and temperature of 6.0 and 30°C, respectively. The enzyme prefers native DNA on denatured DNA Discussion: The catalytic properties of the purified enzyme are essentially the same as those of DNase II family such as independency of divalent ions which inhibited its activity at 10mM. The enzyme acts on dsDNA and ssDNA and generates 3'-phosphate and 5'-OH termini which was a characteristic of DNase II. The functions of the intestinal purified DNase from lysosomes were unclear, but lysosomes contained a set of enzymes required for degradation of food, and the enzyme may be necessary for degradation of nucleic acids within food, but small amounts of the enzyme were found in the nuclei and cytoplasm were detected. Several researchers suggested that DNase II family may be active in apoptosis and played a role as a barrier to transfection. The purified was designated SIDNase. Key words: DNase II, small intestine, immunoabsorption, lysosomes, DLAD Conflict of interests: There is no conflict in interest. ÖZETAmaç: İnsan ince bağırsağından (jejunum) asit DNaz II ailesi ile ilintili DNaz izolasyonu. Materyal ve Metod: İzolasyonlar için iki farklı metod uygulanmıştır: Fosfoselüloz CMselüloz ve Sephadex G-200 den oluşan bir kolon serisi ve immünabsorbsiyon tekniği kullanılarak geliştirilen yeni bir prosedür. Sonuçlar: İzole edilen DNaz tek bir polipeptit zincirinden (monomer) oluşan bir endonükleaz olup, moleküler ağırlığı 28-32 kDa, fonksiyonu için optimal ısısı 30°C, pH ise 6.0 dır. Bu enzim natif DNA'yı denatüre DNA'ya tercih etmektedir. Tartışma: İzole edilen enzimin katalitik özellikleri DNaz II ailesine ait enzimler ile temelde aynıdır. Mesela iki değerlikli iyonlardan bağımsız aktivite göstermesi, aktivitesinin 10mM da inhibe olması gibi. Bu enzim dsDNA ve ssDNA üzerinde DNaz II gibi 3'-fosfat ve 5'-OH terminali oluşturur. Bağırsaklardan izole edilen lizozomal DNaz'ın fonksiyonları tam olarak nitelenememiştir ancak lizozomler gıda parçalanması için bir set enzim içermektedir ve gıdada bulunan nükleik asitlerin parçalanması için gerekli olabilirler. Az miktarda enzim çekirdek ve sitoplazmada bulunmuştur. Bazı araştırmacılar DNAz II ailesinin apoptozda aktif olabileceğini ve görevinin transfeksiyonda bariyer oluşturmak olabileceğini öne sürmüşlerdir. İzolat SIDNaz olarak adlandırılmsiyonıştır.

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Detection of Deoxyribonuclease I Along the Secretory Pathway in Paneth Cells of Human Small Intestine

Cited by 29 publications

References 37 publications

Cd1d-dependent regulation of bacterial colonization in the intestine of mice

Cd1d-dependent regulation of bacterial colonization in the intestine of mice

The multifaceted Paneth cell

A Novel Method For Purifying a DNase From Lysosomal Fraction From Human Small Intestine

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