Detection of damage to DNA and antioxidative activity of yeast polysaccharides at the DNA-modified screen-printed electrode

Bučková, M.; Labuda, Ján; Šandula, J.; Križková, Lı́via; Stĕpánek, I; Ďuračková, Zdena

doi:10.1016/s0039-9140(01)00654-3

Cited by 60 publications

(32 citation statements)

References 23 publications

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“…Electroactive DNA markers have been used to obtain reversible electrode processes at less extreme potentials than those produced by DNA itself (see Table 1) or catalytic signals affording higher sensitivity [35, 43, 52, 54, 59, 79, 82 ± 84]. Some covalent (e.g., osmium tetroxide complexes [32 ± 35, 52]) or non-covalent (e.g., metallointercalators [59,79,83,84]) redox markers exhibit selectivity to DNA structure. Redox mediators (e.g., chelates of ruthenium ions) have been employed to shuttle electrons from DNA G residues to the surface of carbon or (capacitive) DNA structure-sensitive signals obtained at mercury electrodes.…”

Section: Dna Electrochemistrymentioning

confidence: 99%

“…A relatively deep degradation of DNA (e.g., due to acid hydrolysis [99,100], oxidative DNA damage mediated by copper complexes [83] or chemical damage by styrene oxide [81]) is therefore required to observe significant increases in intensity of DNA signals at carbon electrodes. Labuda et al proposed a technique using an intercalative redox intercalator, [Co(phen) 3 ] 3 , for detection of DNA damage [59,60,79,83,101]. Damage to dsDNA at the surface carbon paste electrode (CPE) due to interaction with, e.g., copper chelates mediating generation of reactive oxygen species [83], was accompanied by disruption of its double-helical structure.…”

Section: Detection Of Dna Degradation At Solid Electrodesmentioning

confidence: 99%

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Electrochemical Sensors for DNA Interactions and Damage

2002

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Section: Dna Electrochemistrymentioning

confidence: 99%

Section: Detection Of Dna Degradation At Solid Electrodesmentioning

confidence: 99%

Electrochemical Sensors for DNA Interactions and Damage

2002

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“…14,15,[27][28][29][30] Jaehrig et al 28 verificaram que o potencial antioxidante da parede celular de levedura deve-se menos à glucana que às proteínas da parede celular, especialmente proteínas que apresentam aminoácidos aromáticos ou grupos tiol.…”

Section: Fraçãounclassified

Isolamento de polímeros da parede celular de Saccharomyces cerevisiae e avaliação da atividade antioxidante da manana-proteína isolada

Ferracini-Santos¹,

Sato²

2009

Quím. Nova

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Recebido em 31/1/08; aceito em 25/8/08; publicado na web em 26/1/09 ISOLATION OF POLYMERS FROM Saccahromyces cerevisiae CELL WALL AND EVALUATION OF ANTIOXIDANT ACTIVITY OF ISOLATED MANNAN-PROTEIN. Yeast cell wall contains polymers glucan and mannan-protein that have received much attention with respect to their biological activities. Conventional isolation process involving treatments with hot alkali and acids cause degradation of these polymers. The aim of this paper was to study a low-degrading process for the isolation of glucan and mannan-protein from S. cerevisiae cell wall comprising physic and enzymatic treatments. Yeast cell glucan was obtained in a purity of 87.4% and a yield of 33.7%. The isolated mannan-protein presented antioxidant activity that was increased after thirty minutes of protease treatment. Antioxidant activity was determined by β-carotene/linoleate model system. Keywords: yeast glucan; antioxidant activity; mannan-protein. IntroduçãoA parede celular da levedura Saccharomyces cerevisiae é organizada em duas camadas que são compostas por três macromoléculas principais: manana-proteína, um complexo no qual o polissacarídeo manana está covalentemente ligado à proteína; glucana, um polissacarídeo de β-1,3 e β-1,6 glicose e, quitina, um polímero de β-1,4 N-acetilglicosamina. [1][2][3][4] Estes componentes estão unidos por ligações covalentes. A manana-proteína corresponde a aproximadamente 30% do peso seco da parede celular, 5 e compõe a camada mais externa da parede, estando ligada covalentemente a cadeias de β-1,6-glucana. A β-1,6-glucana corresponde à cerca de 5% do peso seco da parede celular e são moléculas relativamente pequenas, com cerca de 140 resíduos de glicose. Mais da metade da parede celular (50-60%) é formada por β-1,3-glucana, 6 que é composta predominantemente por moléculas lineares com cerca de 1500 resíduos de glicose, dos quais cerca de 40 a 50 resíduos estão envolvidos em ligações através do carbono C-6 com moléculas de β-1,6-glucana e quitina. 7 A β-glucana é encontrada também na parede celular de fungos, em cereais como aveia, trigo, centeio, milho, entre outros. 8 Os estudos sobre a composição das β-glucanas tiveram grande avanço após a identificação de problemas em filtros utilizados nos processos de produção de cerveja, onde se observou o aparecimento de um material gelatinoso. Este material foi identificado como o polissacarídeo β-glucana. 9 As β-1,3-glucanas apresentam habilidade para estimular o sistema imunológico. 5,10 Devido a esta função biológica, esse polissacarídeo pode exercer efeito benéfico contra uma série de doenças, como infecções virais, bacterianas e fúngicas, além de tumores, efeitos de radiações e supressão imune devido ao stress. 11 O problema que as glucanas apresentam e que inviabiliza sua utilização em alguns casos é sua insolubilidade. Por isso, alguns processos de derivação, como carboximetilação e sulfoetilação, e alguns processos físicos, como tratamento com ultrassom, têm sido propostos com o objetivo de aumentar a solubilidade das glucana...

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“…The strategy is based on the employment of DNA itself as the sensor biocomponent for the detection of species leading to damage or protection of DNA. Mercury as well as solid electrodes were used as electrochemical transducers of a signal [8][9][10][11]. Electrically heated electrodes represent a way of enhancement of the sensitivity of electrochemical analysis [12,13].…”

Section: Introductionmentioning

confidence: 99%

Voltammetric Detection of Antioxidative Properties of Flavonoids Using Electrically Heated DNA Modified Carbon Paste Electrode

Korbut

Bučková

Labuda

et al. 2003

Sensors

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Abstract:A simple electrochemical sensor consisting of electrically heated carbon paste electrode with the surface modified by dsDNA is used to characterize voltammetric behaviour and antioxidative activity of four selected flavonoids. Quercetin, rutin, catechin and epigallocatechin gallate accumulate within the DNA layer. A positive effect of the electrode temperature within the range of 20 to 38 ºC on the detection of a deep DNA degradation by a copper(II)/H 2 O 2 /ascorbic acid cleavage mixture as well as an antioxidative effect of flavonoids was evaluated.

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Detection of damage to DNA and antioxidative activity of yeast polysaccharides at the DNA-modified screen-printed electrode

Cited by 60 publications

References 23 publications

Electrochemical Sensors for DNA Interactions and Damage

Electrochemical Sensors for DNA Interactions and Damage

Isolamento de polímeros da parede celular de Saccharomyces cerevisiae e avaliação da atividade antioxidante da manana-proteína isolada

Voltammetric Detection of Antioxidative Properties of Flavonoids Using Electrically Heated DNA Modified Carbon Paste Electrode

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