Detection of conjugated C16 PUFAs in rat tissues as possible partial beta-oxidation products of naturally occurring conjugated linoleic acid and its metabolites

Banni, Sebastiano; Petroni, A.; Blasevich, M.; Carta, Gianfranca; Angioni, Elisabetta; Murru, Elisabetta; Day, Billy W.; Melis, Maria Paola; Spada, Simona; Ip, Clement

doi:10.1016/j.bbalip.2004.03.003

Cited by 47 publications

(30 citation statements)

References 45 publications

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“…Detailed investigations of the concentration of stearic and palmitic acids in the rat liver reinforce the findings of this study and of Banni et al (2004) showing that the c9t11 isomer and t10c10CLA, in particular, decrease the yield of cis-9 fatty acids formation (Table 3). Consequently, the concentrations of these saturated fatty acids are higher in the liver of rats fed the diet enriched in these isomers, irrespective of the presence of Se, compared with the controls.…”

Section: The Effect Of the Diets On The Concentration Of Cla Isomers supporting

confidence: 63%

“…Considering the above evidence, we decided to investigate whether a diet enriched in Se-yeast stimulated the accumulation of conjugated linoleic acid (CLA) isomers in rat livers. CLA isomers are believed to play a principal role in many important physiological functions and may lead to changes in body composition, body fat reduction, overall energy expenditure or modulate immune function (Banni et al, 2004;Rainer and Heiss, 2004). CLA isomers are also expected to inhibit tumorigenesis by interfering in the metabolism of some carcinogens (Wahle et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Influence of dietary conjugated linoleic acid isomers and selenium on growth, feed efficiency, and liver fatty acid profile in rats

Korniluk¹,

Czauderna²,

Kowalczyk³

et al. 2006

J. Anim. Feed Sci.

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The influence of dietary CLA isomer(s) and/or selenized yeast on the growth, concentration of CLA isomers and other fatty acids in the liver was investigated in rats. Plasma blood triacylglycerols (TAG), total cholesterol (TC), HDL and LDL cholesterol fractions in relation to dietary CLA isomer(s) and/or selenium (Se) were analysed. The experiment was performed on female rats (Wistar), 8 weeks of age and initial body weight of about 200 g. After a 1-week preliminary period, for 4 weeks the animals were fed a diet enriched in conjugated linoleic acid (CLA) isomer(s) and selenized yeast (2×2 experimental design). Dietary Se or/and CLA isomer(s) resulted in small changes in the spleen, heart, kidneys and brain, and increased liver weight. Administration of Se and trans10cis12CLA most efficiently increased the body weight gain of rats. CLA isomer(s) administered with or without Se elevated the CLA isomer(s) level in the liver. These results demonstrate that trans,transCLA isomers are metabolized more slowly, while cis,trans/trans,cisCLA isomers, more rapidly to longchain fatty acids containing a conjugated double bond. Enrichment of the diet in CLA isomer(s) with or without Se caused a reduction in the capacity of Δ9-, Δ6-and Δ5-desaturases in the liver, while dietary trans10cis12CLA or the CLA isomer mixture increased Δ4-desaturases. The contents of oleic acid, C20:4n-6, and C20:5n-3 decreased in the liver, whereas the level of C22:5n-3 and C22:6n-3 increased in the liver of rats fed the CLA isomer mixture. Individual dietary CLA isomers with or without Se increased the concentration of palmitic and stearic acids in the liver. All experimental diets increased the concentration of triacylglycerols in blood plasma, while trans10cis12CLA with or without Se usually decreased the concentration of total cholesterol, LDL, and HDL cholesterol.

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Section: The Effect Of the Diets On The Concentration Of Cla Isomers supporting

confidence: 63%

Section: Introductionmentioning

confidence: 99%

Influence of dietary conjugated linoleic acid isomers and selenium on growth, feed efficiency, and liver fatty acid profile in rats

Korniluk¹,

Czauderna²,

Kowalczyk³

et al. 2006

J. Anim. Feed Sci.

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“…Expression in the by guest, on May 11, 2018 www.jlr.org The 75 kDa band was most prominent in adipose tissue. In addition, much of the immunoreactive D2 migrated as a prominent smear in our SDS-PAGE gels, ranging from 120 to >220 kDa, the largest of our molecular weight markers.…”

Section: Development Of Abcd2 Antibodymentioning

confidence: 99%

ABCD2 is abundant in adipose tissue and opposes the accumulation of dietary erucic acid (C22:1) in fat

Liu¹,

Sabeva²,

Bhatnagar

et al. 2010

Journal of Lipid Research

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This article is available online at http://www.jlr.orgPeroxisomes play essential roles in the metabolism of both dietary and endogenously synthesized lipids ( 1, 2 ). The synthesis of the etherphospholipids (platelet activating factor and plasmalogens), partial ␤ -oxidation of verylong-chain fatty acids (VLCFAs; C>22), and the ␣ -oxidation of phytanic acid occur within this organelle. A comprehensive integrative map of peroxisomal metabolic pathways can be found at www.peroxisomedb.org ( 3 ). The importance of the organelle is best exemplifi ed by the occur rence of peroxisome biogenesis disorders and singleenzyme defi ciencies that result in severe metabolic diseases that are often lethal in early childhood. X-linked adrenoleukodystrophy (X-ALD) is the most common peroxisomal disorder characterized by the accumulation of VLCFA in tissues, progressive demyelination, and adrenocortical dysfunction ( 4 ). X-ALD is caused by mutations in ABCD1 (D1), an ATP binding cassette (ABC) half transporter that presumably mediates the transport of very-long-chain acylCoAs into peroxisomes ( 5 ). D1 is the founding member of a quartet of ABC half-transporters that contain peroxisomal targeting sequences and form heterodimers in vitro ( 6 ). However, homodimers appear to be preferred in vivo, and each subfamily member has a unique tissue distribution ( 7,8 ).The closest paralog to D1 is D2, which shares 66% amino acid identity and is expressed in the adrenal, brain, and liver ( 9 ). Expression of a D2 transgene in D1-defi cient mice partially corrected the phenotype in this mouse Abstract The ATP binding cassette transporter, ABCD2 (D2), is a peroxisomal protein whose mRNA has been detect ed in the adrenal, brain, liver, and fat. Although the role of this transporter in neural tissues has been studied, its function in adipose tissue remains unexplored. The level of immunoreactive D2 in epididymal fat is >50-fold of that found in brain or adrenal. D2 is highly enriched in adipocytes and is upregulated during adipogenesis but is not essen tial for adipocyte differentiation or lipid accumulation in day 13.5 mouse embryonic fi broblasts isolated from D2-defi cient (D2 ؊ / ؊ ) mice. Although no differences were appreci ated in differentiation percentage, total lipid accumulation was greater in D2 ؊ / ؊ adipocytes compared with the wild type. These results were consistent with in vivo observa tions in which no signifi cant differences in adiposity or adipocyte diameter between wild-type and D2؊ / ؊ mice were observed. D2

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“…50.1−51.4 min and 53.6−55.3 min) . Considering the above, as well as the UV spectra of these species having an absorbance maximum at 234 nm Banni et al, 2004), we argue that these fatty acids containing conjugated double bonds (CFA) contained more than two double bonds because of their shorter elution times compared with the retention times of the CLA isomers possessing two conjugated double bonds. Studies by Gnädig et al (2001) and our recent studies carried out on rats also reinforce the finding that CLA isomers could be metabo- LO = linseed oil, SE = sodium selenate, LOSE = linseed oil + sodium selenate, DM = dry matter 1 mean values in columns having the same superscripts are significantly different at a,b P < 0.05 and A,B P < 0.01; differences at α,β P = 0.1 are indicated as tendencies; interactions were significant at the x,y P < 0.05 and X,Y P < 0.01 levels, respectively lized in vivo into long-chain conjugated PUFA (via elongation and desaturation products of CLA isomers) using the same pathway as linoleic acid (LA).…”

Section: Effect Of the Diet Enriched With Lo And Sevi On Cla Isomer Pmentioning

confidence: 99%

Dietary linseed oil and selenate affect the concentration of fatty acids in selected tissues of sheep

Czauderna¹,

Kowalczyk²,

Marounek³

2012

CZECH J ANIM SCI

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ABSTRACT:The aim of the study was to determine the influence of the addition of 5% linseed oil (LO) and 2 µg Se as selenate (SeVI) per g of diet on body mass gain (BMG), feed conversion efficiency (FCE), and concentrations of fatty acids (FA), especially conjugated linoleic acid (CLA) isomers, in the liver, heart, m. longissimus dorsi (MLD), m. biceps femoris (MBF), subcutaneous fat (SF), perirenal fat (PF), and intermuscular fat (IF) of sheep. Each group comprised 5 lambs with an average initial body weight of 25 ± 2 kg. For 35 days the sheep were fed the control or experimental diets enriched with 5% LO, SeVI, or LO and SeVI combined. The diets with LO, regardless of the presence of SeVI, increased BMG, FCE, and the mass of MBF, MLD, and the liver. The LO treatment increased the concentration of total CLA isomers in the liver, SF, PF, and MLD in comparison with the control. Dietary LO with or without SeVI resulted in negligible changes in the concentration of total CLA isomers in MBF. The diet enriched with LO, irrespective of the presence of SeVI, increased the concentrations of cis-monounsaturated fatty acids (cis-MUFA), polyunsaturated fatty acids (PUFA), as well as unsaturated fatty acids (UFA) in the liver, heart, and especially in SF and PF (i.e. energy storage tissues) compared with the control group and SeVI-fed sheep. Dietary SeVI more effectively increased the capacity of ∆9-desaturation in all adipose tissues as the concentration of cis9,trans11CLA increased in these tissues. The addition of SeVI to the diet decreased the yield of the catabolism of fatty acids in these adipose tissues and both muscles compared with the control sheep or other experimental groups. The diets enriched with LO with or without SeVI increased the nutritional value of MBF, MLD, and adipose tissues as the ratio of unsaturated FA/saturated FA increased. Our results of a short-term study provide useful information for nutritionists carrying out further investigations to improve the nutritional quality of feed for ruminants as well as humans.

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Detection of conjugated C16 PUFAs in rat tissues as possible partial beta-oxidation products of naturally occurring conjugated linoleic acid and its metabolites

Cited by 47 publications

References 45 publications

Influence of dietary conjugated linoleic acid isomers and selenium on growth, feed efficiency, and liver fatty acid profile in rats

Influence of dietary conjugated linoleic acid isomers and selenium on growth, feed efficiency, and liver fatty acid profile in rats

ABCD2 is abundant in adipose tissue and opposes the accumulation of dietary erucic acid (C22:1) in fat

Dietary linseed oil and selenate affect the concentration of fatty acids in selected tissues of sheep

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