Detection of Colistin Susceptibility in Multi-Drug Resistant Pseudomonas Aeruginosa and Acinetobacter Baumannii by Four Different Methods

Kandeel, Amina

doi:10.12816/0036990

Cited by 3 publications

(3 citation statements)

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“…As previously reported by Somily et al 15 , most of the MDR isolates in this study were obtained from ICU patients (76.67%). This can be explained by several factors that predispose ICU patients to bacterial infections such as the use of invasive devices, severity of diseases, prolonged duration of hospitalization, comorbidities, and the higher use of broad-spectrum or multiple antibiotics.…”

Section: Discussionsupporting

confidence: 71%

Resistance to Colistin Mediated by mcr-1 among Multidrug Resistant Gram Negative Pathogens at a Tertiary Care Hospital, Egypt

Shabban¹,

Fahim²,

Montasser³

et al. 2020

J Pure Appl Microbiol

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Colistin is considered the last option for treatment of infections caused by Multidrug Resistant (MDR) Gram negative pathogens. The mcr-1 gene could transfer the resistance to colistin between different species. Therefore, screening of this resistance mechanism will help greatly in the control of further spread of colistin resistance and enhancing the outcomes of patients. The current work aimed to study the frequency of colistin resistance among MDR Gram negative pathogens isolated from clinical samples at Ain Shams University Hospitals and to explore if the mcr-1 gene was the responsible mechanism for this resistance. These pathogens were isolated from various samples including; blood, pleural fluid, sputum, urine, swabs from surgical and burn wounds, that were submitted from wards and intensive care units to the central microbiology laboratory at Ain Shams University Hospital, Cairo, Egypt, during the period from June to December 2019. Culture and bacterial identification were done by conventional microbiologic techniques. Eighty Gram negative bacterial pathogens were assayed for antimicrobial susceptibility by disc diffusion test. Sixty MDR Gram negative isolates were identified and further studied for colistin susceptibility by E-test, as well as real-time PCR to detect mcr-1 gene. Totally four isolates (6.7%) were phenotypically resistant to colistin. We found mcr-1 gene in three of the obtained isolates (5%); 1 Pseudomonas aeruginosa (5.3%) and 2 Acinetobacter baumannii (14.3%). In conclusion, although we detected a low prevalence of mcr-1 positive isolates from human clinical samples, continuous monitoring and implementation of infection control precautions are greatly required, to interfere with the further occurrence and transfer of bacterial species carrying the mcr-1 gene.

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Section: Discussionsupporting

confidence: 71%

Resistance to Colistin Mediated by mcr-1 among Multidrug Resistant Gram Negative Pathogens at a Tertiary Care Hospital, Egypt

Shabban¹,

Fahim²,

Montasser³

et al. 2020

J Pure Appl Microbiol

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“…Lastly, all strains were sensitive to colistin with MICs not exceeding 1 μg/ml. Our results were similar to those obtained by Kandeel [36], who stated the MIC of colistin as less than 2 μg/ml.…”

Section: Antibiotic Resistance Profile Of Acinetobacter Baumannii Strainssupporting

confidence: 92%

Antibiotic Resistance Pattern of Acinetobacter baumannii Strains Isolated from Different Clinical Specimens and Their Sensibility Against Bioactive Molecules Produced by Actinobacteria

et al. 2019

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Acinetobacter baumannii frequently targets the most vulnerable hospitalized patients, especially in intensive care units. Bacteremia, nosocomial pneumonia and surgical wound infections caused by A. baumannii have a higher mortality compared to other nosocomial pathogens. The severity of the diseases caused by this microorganism is accentuated by its resistance to many antibiotics currently used in the world. In this context, we undertook to isolate several A. baumannii strains from various samples collected in the Béni Messous University Hospital Center, Algiers (Algeria), to determine the antibiotic resistance profile of these strains and to evaluate the antagonistic potential of some actinobacterial strains against A. baumannii. Nineteen A. baumannii strains were isolated and identified. The predominant number of strains isolated from pulmonary samples (36.84%) and from the intensive care unit (57.89%) was A. baumannii. All A. baumannii strains were sensitive to colistin but exhibited high resistance to 11 other antibiotics tested. Of the 19 strains, 15 were metallo-β-carbapenemase producers, 10 were cephalosporinase, and three were extended-spectrum β-lactamases (ESBL) producers. Of 59 strains of actinobacteria tested, 19 (32.20%) showed activity against at least one strain of A. baumannii, the highest activity being detected in strains belonging to the genus Saccharothrix (or related genera). However, Streptomyces sp. WAB9 had the widest spectrum of activity. Based on the obtained results, colistin remains the unique antibiotic which inhibits the growth of all A. baumannii strains, and some actinobacterial strains also present an alternative source of new molecules with strong bioactivities against multidrug-resistant A. baumannii strains.

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“…In the same line, Nowak et al 23 , reported that colistin resistanc was 47.7% among isolated A. baumannii from patients with ventilatorassociated pneumonia in Greece, Italy and Spain, whereas Al-Kadmy et al 24 in Iraq, reported higher rate (76%). In contrary, Hameed et al 25 noted lower rates in Pakistan (9.6%) and Kandee 26 , in Egypt (2.8%).…”

Section: Discussionmentioning

confidence: 89%

Evaluation of potential synergistic activity of antimicrobial combinations against Colistin resistant Acinetobacter baumannii

Dawoud

Saied

Salem

2023

Egyptian Journal of Medical Microbiology

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Background: Acinetobacter baumannii (A. baumannii) is one of the most important nosocomial pathogen. With the introduction of colistin as a last resort in treatment of A. baumannii, resistant strains. Using combined antibiotics could increase the success of treatment and reduce resistance. Objectives: To assess potential in-vitro synergistic activity of colistin when combined with (vancomycin, teicoplanin, rifampicin, tigecycline, meropenem, amikacin, and ceftazidime) against colistin resistant A. baumannii (CRAB). Also, to screen the prevalence of plasmid mediated colistin resistance (mcr) genes (mcr-1 to mcr-5) in colistin resistant isolates. Methodology: A. baumannii strains were isolated from different microbial specimens. Antibiotic susceptibility testing was done for all isolates by disk diffusion method while broth micro dilution (BMD) was performed to determine colistin minimum inhibitory concentration (MIC). Genotypic detection of mcr genes was done using multiplex PCR. Checkerboard method was done to detect potential synergistic activities between colistin and other tested antibiotics against CRAB. Results: A total of 94 A. baumannii strains were isolated from 373 different clinical samples. Colistin resistance was detected in 44/94 (46.8%) of isolated A. baumannii. Only 2 out of 44 CRAB (4.5%) carried mcr-1 gene and neither of other mcr genes could be detected. All isolated CRAB were resistant to 7 tested antibiotics by BMD. But when colistin was included in a checkerboard pattern, colistin-based combinations with vancomycin, meropenem, rifampicin, teicoplanin and ceftazidime showed synergy in 93. 2%, 90.9%, 88.7%, 86.4% and 79.5% of isolated CRAB respectively. For both tigecyclin and amikacin more than 90% of CRAB showed indifference in combination with colistin. Conclusion: Further studies are needed to determine the ability of colistin based antimicrobial combinations as an alternative therapy to treat CRAB infections and confirm that synergy.

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Detection of Colistin Susceptibility in Multi-Drug Resistant Pseudomonas Aeruginosa and Acinetobacter Baumannii by Four Different Methods

Cited by 3 publications

References 0 publications

Resistance to Colistin Mediated by mcr-1 among Multidrug Resistant Gram Negative Pathogens at a Tertiary Care Hospital, Egypt

Resistance to Colistin Mediated by mcr-1 among Multidrug Resistant Gram Negative Pathogens at a Tertiary Care Hospital, Egypt

Antibiotic Resistance Pattern of Acinetobacter baumannii Strains Isolated from Different Clinical Specimens and Their Sensibility Against Bioactive Molecules Produced by Actinobacteria

Evaluation of potential synergistic activity of antimicrobial combinations against Colistin resistant Acinetobacter baumannii

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