Detection of Colistin Resistance Gene
            <i>mcr-1</i>
            in Hypervirulent Klebsiella pneumoniae and Escherichia coli Isolates from an Infant with Diarrhea in China

Gu, Danxia; Huang, Yonglu; Ma, Jian-Gang; Zhou, Hongwei; Fang, Ying; Cai, Jiachang; Hu, Yanyan; Zhang, Rong

doi:10.1128/aac.00476-16

Cited by 53 publications

(30 citation statements)

References 14 publications

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“…This study was the first testing of increasing Klebsiella, which belonged to Proteobacteria in RD infants. More recent studies suggested Klebsiella may contribute to inflammation (33,34), such as bronchitis, pneumonia, and meningitis (35). Therefore, it could be supposed that RD infection, as the major cause of the viral gastroenteritis might disrupt the gut microbiota and introduce more effects from Klebsiella.…”

Section: Discussionmentioning

confidence: 99%

Fecal Microbiota, Lactic Acid and Short Chain Fatty Levels of Infants Following Rotavirus Infection Revealed by Illumina Miseq High-Throughput Sequencing and HPLC Method

Huang

Nevin

et al. 2019

Jundishapur J Microbiol

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Background: Rotavirus (RV) is one of the major causes of acute gastroenteritis in infants. It is indispensable to demonstrate the relationship between the diversity and richness of gut microbiota and RV infection using more accurate and effective technology. Objectives: To investigate the differences in fecal microbiota, lactic acid, and short-chain fatty acids (SCFAs) levels between rotaviralinduced diarrhea (RD) infants and healthy (H) infants. Methods: The infants comprised of 25 infants aged few days to six months, who were in good health (n = 12) or diagnosed with rotavirus (n = 13). Fecal matter was analyzed with Illumina Miseq high-throughput sequencing technique targeting the 16s rRNA gene V3-V4 region. Lactic acids and SCFAs were measured by the high-performance liquid chromatography (HPLC) technique. Results: Compared to H infants, the fecal samples in RD infants had lower Shannon diversity index and the bacteria richness (P < 0.05). A higher proportion of Proteobacteria, Enterobacteriaceae and Klebsiella, and lower abundances of Actinobacteria and Knoellia (P < 0.05) were detected in fecal samples of RD infants. The total SCFAs content of fecal samples showed no distinction between RD and H infants, yet lower levels of lactic acid were observed in fecal samples of RD infants. Conclusions: Rotaviral infection in infants led to an alteration of fecal microbiota and lactic acid concentration compared with healthy infants. Fecal microbiota and metabolite may advance the understanding and treatment of RD.

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Section: Discussionmentioning

confidence: 99%

Fecal Microbiota, Lactic Acid and Short Chain Fatty Levels of Infants Following Rotavirus Infection Revealed by Illumina Miseq High-Throughput Sequencing and HPLC Method

Huang

Nevin

et al. 2019

Jundishapur J Microbiol

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“…As a consequence, Klebsiella is now considered a reservoir for antimicrobial resistance and virulence genes (Holt et al, 2015 ). The acquisition and dissemination of ESBL-, carbapenem- and now colistin-resistance encoding genes (Gu et al, 2016 ; Ridolfo et al, 2016 ) makes this pathogen an urgent threat to human health. In this study, a collection of K. pneumoniae isolates resistant to ESBL- and FQ- compounds were further characterized.…”

Section: Discussionmentioning

confidence: 99%

Exploring the Genome and Phenotype of Multi-Drug Resistant Klebsiella pneumoniae of Clinical Origin

et al. 2017

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Klebsiella pneumoniae is an important nosocomial pathogen with an extraordinary resistant phenotype due to a combination of acquired resistant-elements and efflux mechanisms. In this study a detailed molecular characterization of 11 K. pneumoniae isolates of clinical origin was carried out. Eleven clinical isolates were tested for their susceptibilities, by disk diffusion and broth microdilution and interpreted according to CLSI guidelines. Efflux activity was determined by measuring the extrusion of ethidium bromide and biofilm formation was assessed following static growth in Müeller-Hinton and minimal media M9 broths at two temperatures and time points. Template DNA from all 11 isolates was extracted and sequenced. The study collection was found to be resistant to several (extended-spectrum beta-lactam) ESBL-type compounds along with several (fluoro)quinolones (FQ). Resistance to tetracycline accounted for 55% of the study collection (n = 6) and three of the 11 isolates were resistance to carbapenems. Genotyping identified blaCTX-M-15 (82%), blaSHV-12 (55%), and blaTEM-1B (45%) ESBL encoding genes and FQ resistance was associated the presence of the oqxAB operon, identified in 10 of the 11 isolates and qnrB gene in one isolate. The polymorphisms detected in the quinolone resistance-determining regions (QRDRs) were associated with isolates of the clonal group CG15. Sequence types (ST) identified were representative of previously described clonal groups including CG258 (n = 7), CG15 (n = 3), and CG147 (n = 1). Plasmid replicon type databases were queried indicating the presence of IncFII and IncFIB replicon types in the majority of the isolates (91%), followed by IncFIA (45%), and IncR (45%). Two of the 11 isolates were found positive for yersiniabactin siderophore-encoding genes. No differences in the ability to efflux ethidium bromide were identified. Biofilm formation was stronger when the isolates were grown under stressed conditions at 37°C for a period up to 96 h. These data confirm the fact that well-recognized clonal groups of K. pneumoniae of importance to human health carries a diverse repertoire of antimicrobial resistance determinants, particularly related to critically important drugs in the ESBL and FQ classes. The capacity of most isolates to form strong biofilms, when stressed under laboratory-simulated conditions, supports the risk to human health associated with nosocomial infections deriving from indwelling medical devices.

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“…This subtype is non-resistant to most of the commonly used antimicrobials such as colistin and carbapenems. But the recent reports of carbapenem-resistant hvKp strains which belong to the sequence types 11 (ST11) [7], ST25 and ST65 [8] poses a major clinical concern. HvKp strains can cause serious infections in both immunocompetent, diseased and healthy young individuals [9].…”

Section: Klebsiella Pneumoniae: the Classical And Other Subtypesmentioning

confidence: 99%

Klebsiella pneumoniae: an increasing threat to public health

Effah

Sun

Liu

et al. 2020

Ann Clin Microbiol Antimicrob

290

225

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Objectives: This review fills the paucity of information on K. pneumoniae as a nosocomial pathogen by providing pooled data on epidemiological risk factors, resistant trends and profiles and resistant and virulent genes of this organism in Asia. Methods:Exhaustive search was conducted using PubMed, Web of Science, and Google scholar for most studies addressing the prevalence, risk factors, drug resistant-mediated genes and/or virulent factors of K. pneumoniae in Asia. Data extracted for meta-analysis were analyzed using comprehensive meta-analysis version 3. Trends data for the isolation rate and resistance rates were entered into Excel spread sheet and the results were presented in graphs. Results: The prevalence rate of drug resistance in K. pneumoniae were; amikacin (40.8%) [95% CI 31.9-50.4], aztreonam (73.3%) [95% CI 59.9-83.4], ceftazidime (75.7%) [95% CI 65.4-83.6], ciprofloxacin (59.8%) [95% CI 48.6-70.1], colistin (2.9%) [95% CI 1.8-4.4], cefotaxime (79.2%) [95% CI 68.0-87.2], cefepime (72.6) [95% CI 57.7-83.8] and imipenem (65.6%) [95% CI 30.8-89.0]. TEM (39.5%) [95% CI 15.4-70.1], SHV-11 (41.8%) [95% CI 16.2-72.6] and KPC-2 (14.6%)[95% CI 6.0-31.4] were some of the resistance mediated genes observed in this study. The most virulent factors utilized by K. pneumoniae are; hypermucoviscous phenotype and mucoviscosity-related genes, genes for biosynthesis of lipopolysaccharide, iron uptake and transport genes and finally, adhesive genes. Conclusion:It can be concluded that, antimicrobial resistant in K. pneumoniae is a clear and present danger in Asia which needs strong surveillance to curb this menace. It is very important for public healthcare departments to monitor and report changes in antimicrobial-resistant isolates.

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Detection of Colistin Resistance Gene mcr-1 in Hypervirulent Klebsiella pneumoniae and Escherichia coli Isolates from an Infant with Diarrhea in China

Cited by 53 publications

References 14 publications

Fecal Microbiota, Lactic Acid and Short Chain Fatty Levels of Infants Following Rotavirus Infection Revealed by Illumina Miseq High-Throughput Sequencing and HPLC Method

Fecal Microbiota, Lactic Acid and Short Chain Fatty Levels of Infants Following Rotavirus Infection Revealed by Illumina Miseq High-Throughput Sequencing and HPLC Method

Exploring the Genome and Phenotype of Multi-Drug Resistant Klebsiella pneumoniae of Clinical Origin

Klebsiella pneumoniae: an increasing threat to public health

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