Detection of Coliforms and Enteric Pathogens in Favorite Snack Food Sold in Yogyakarta City

Budiarso, Tri Yahya; Amarantini, Charis; Prihatmo, Guruh; Restiani, Ratih; Putri, Yesika; Kindagen, Virgin; Linggardjati, Sharoneva

doi:10.2991/assehr.k.210305.007

Cited by 3 publications

(2 citation statements)

References 24 publications

(32 reference statements)

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“…An investigation of ready-to-eat food in public places of Yogyakarta in Indonesia showed several enteropathogens. Apart from the entero pathogens mentioned above, some other pathogens found in this study are Yersinia enterocolitica, Pantoea species, Aeromonas hydrophila, Serratia species, Proteus mirabilis, and Ewangella americana 11 . Contamination due to poor hygiene and poor temperature control while preparing food is one of the root causes of transferring enteric pathogens to food.…”

Section: Introductionmentioning

confidence: 73%

Prevalence of Enteric Pathogens in the Prepackaged Salads in Makkah City, Saudi Arabia

Ashgar

Alharthi

Al-Said

et al. 2023

Egyptian Journal of Medical Microbiology

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Background: Ready-to-eat foods are exposed to contamination in several ways either during harvesting, gathering, or processing. Food contamination with pathogens is a serious concern. Objective: The study amis to detect pathogenic bacteria in ready meals especially the prepared salads in restaurants in Makkah city. Methodology: A total of 110 samples of two types of salads, (55 sliced green salad and 55 crushed salad) were randomly collected from 55 different restaurants. Several types of culture media were used for the isolation of the pathogenic bacteria from salads samples and the identification of pathogenic bacterial strains was done using traditional and automated methods (VITEK2). Results: The current study results showed that Enterococcus faecalis recorded the highest percentage (89%), while Acinetobacter hemolyticus recorded the lowest percentage (1.8 %) among the isolated pathogenic bacterial strains from crushed and sliced green salad samples. Conclusion: This study revealed that most of the salad samples were contaminated with pathogenic bacteria and the highest percentage was recorded among the sliced green salad samples. The high pollution rates indicate a failure to implement health requirements during the preparation of meals in restaurants.

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Section: Introductionmentioning

confidence: 73%

Prevalence of Enteric Pathogens in the Prepackaged Salads in Makkah City, Saudi Arabia

Ashgar

Alharthi

Al-Said

et al. 2023

Egyptian Journal of Medical Microbiology

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“…Furthermore, gram staining was done and then biochemical tests were carried out using API media. API 20E is a standardized identification system which uses 20 miniature tubes or wells for biochemical tests of microorganisms (Budiarso, 2021) Based on the results of research from 5 samples tested with API 20E, it is known that 2. samples show the results of Staphylococcus aureus and E. coli bacteria. Of the 5 samples of food snacks tested with API 20E, it is known that 2 samples are Staphylococcus aureus and E. coli bacteria with the results showing if the positive color and negative color are not the same as the sample color.…”

Section: Table 6 Total Sample Types Of Snacks In Health Facilities Ba...mentioning

confidence: 99%

Bacteriological Analysis of Street Food to Support the Availability of Hygienic Functional Foods in Kendari City

et al. 2023

IJFMR

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Food contaminated by pathogenic bacteria can cause various diseases such as diarrhea and typhoid. Bacterial growth in food can also result in unwanted physical or chemical changes, making the food unfit for consumption. The purpose of this study was to determine the number and type of bacteria in some snacks around health facilities in Kendari City. This type of research is descriptive research, the samples in this study are types of snacks around health facilities in Kendari City with testing methods in the form of Total Plate Numbers, API Test, and Confirmation test. The results showed that the total plate number contamination of 2 out of 5 food samples amounted to 3x103 - 4x103 colonies/g, namely in samples 2 and 3. These results indicate that the total plate number value in these samples is higher than SNI 7388: 2009 which is < 1x102 colonies/g. The number of colonies in sample 1, namely fried sweet potato, amounted to 0, in sample 2, namely fried banana, amounted to 4x103, in sample 3, namely sweet potato, amounted to 3x103, in sample 4, namely tempeh, amounted to 0 and sample 5, namely bakwan, amounted to 0. The type of bacteria in the results of bacterial identification testing using the API method in sample 2 is Staphylococcus aureus and sample 3 is E.coli.

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Microbiological quality of ready-to-eat street foods in Lisbon, Portugal

Barreira,

Marcos,

Flores

et al. 2024

Discov Food

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Street food sector is an important component of the food distribution system in many cities. However, foods exposed for sale on the roadside are generally associated with conditions that favors its potential microbiological contamination. In this study, the microbiological quality of 118 ready-to-eat street foods sold in the metropolitan area of Lisbon, Portugal was evaluated. Mesophilic aerobic bacteria, yeasts, moulds, Enterobacteriaceae and Escherichia coli were used as spoilage/hygiene indicators. Listeria monocytogenes, Salmonella spp., coagulase-positive staphylococci (CPS), Clostridium perfringens, Bacillus cereus and E. coli pathotypes implicated in gastrointestinal disease were used as food safety/hygiene indicators. Thirty-five (29.7%) of the samples were classified as satisfactory, 29 (24.6%) as questionable and 51 (43.2%) as unsatisfactory. Three samples (2.6%) were unsatisfactory/ potentially dangerous, due to the presence of the L. monocytogenes > 102 cfu/g and/or B. cereus > 105 and/or CPS > 104. B. cereus genes encoding causative toxins were detected in the strains isolated from two samples containing > 105 cfu/g. Salmonella spp., Clostridium perfringens and pathogenic E. coli were not detected in any of the examined samples. CPS was detected in 26.3% of the samples.A significant relationship between microbiological quality and food groups, with the increase of questionable and unsatisfactory classifications for those samples including raw components, like fruits and vegetables, was observed (Fisher-Freeman-Halton = 29.01, p < 0.001). The obtained data highlights for the need of education and training of street-food vendors to improve the food safety management system in the place of sale.

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Detection of Coliforms and Enteric Pathogens in Favorite Snack Food Sold in Yogyakarta City

Cited by 3 publications

References 24 publications

Prevalence of Enteric Pathogens in the Prepackaged Salads in Makkah City, Saudi Arabia

Prevalence of Enteric Pathogens in the Prepackaged Salads in Makkah City, Saudi Arabia

Bacteriological Analysis of Street Food to Support the Availability of Hygienic Functional Foods in Kendari City

Microbiological quality of ready-to-eat street foods in Lisbon, Portugal

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