Detection of classical swine fever virus in archival formalin-fixed tissues by reverse transcription-polymerase chain reaction

Singh, Vinod; Kumar, G. Sai; Paliwal, O. P.

doi:10.1016/j.rvsc.2004.11.002

Cited by 8 publications

(5 citation statements)

References 9 publications

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“…Similar to present study, several authors (Liu et al, 1991;Singh et al, 2005;Rathnapraba et al, 2012Rathnapraba et al, & 2013 also detected CSF viral nucleic acid in tissue samples such as liver, spleen, kidney and mesenteric lymphnodes by RT-PCR. All the samples were proved to be CSF positive.…”

Section: Resultssupporting

confidence: 89%

An Outbreak of Classical Swine Fever in Indigenous Pigs in Tamilnadu, India

Malmarugan¹,

Sundaram²,

Rajeswar³

2014

IJAVST

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Classical swine fever is a highly contagious viral disease of swine causing economic losses due to heavy mortality and reproductive problems. The present study was conducted at Nakalapuram village, Villathikulam taluk of Thoothukudi District, Tamilnadu where there was a suspected outbreak of swine fever among the indigenous pigs reared under scavenging system. Ninety pigs out of one hundred and ten pigs of all age groups without any vaccination died within two to three days period. The ailing animals showed clinical signs such as high fever, staggering gait, frothy excessive salivation, severe respiratory distress, paddling of legs with convulsions followed by death. The post mortem examination was carried out in two pigs and the histopathological examination was carried out on representative necropsy samples like liver, spleen, kidney and mesenteric lymph node. Based on the necropsy findings such as haemorrhagic lymphadenitis, petechial haemorrhages in kidney and spleen and by NS5B gene based RT-PCR the disease was confirmed as classical swine fever.

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Section: Resultssupporting

confidence: 89%

An Outbreak of Classical Swine Fever in Indigenous Pigs in Tamilnadu, India

Malmarugan¹,

Sundaram²,

Rajeswar³

2014

IJAVST

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“…Se ha demostrado también que mediante la técnica de RT-PCR puede detectarse el ARN del VPPC en tejidos fijados en formalina (Ha et al, 2004;Singh et al, 2005), lo que podría ser muy ventajoso ante la sospecha de la ocurrencia de PPC en porcinos de lugares alejados en la sierra y selva del país, o ante la imposibilidad de enviar tejidos frescos al laboratorio, pues las pruebas convencionales de IF o aislamiento viral requieren de tejidos frescos y adecuadamente conservados.…”

Section: Discussionunclassified

Standardization and Validation of Qualitative Real Time Rt-PCR for Detection of Classical Swine Fever Virus

Chiok

Manchego

Rivera

et al. 2012

Rev. investig. vet. Perú

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“…Specific amplification was observed in eight samples for 5 0 UTR target while one of the tissues shows amplification for E2 gene target fragment. Similarly, Singh et al [9] yielded specific amplicons in three out of ten archival samples subjected to RT-PCR targeting 172 bp of E2 gene. Amplification in less number of samples can be attributed to decrease in the sensitivity of RT-PCR due to formalin fixation process [10].…”

mentioning

confidence: 88%

Phylogenetic analysis of Classical swine fever virus from archival formalin fixed clinical tissues reveals vietnamese origin of the isolates

Singh

Rajak

2017

VirusDis.

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Detection of Classical swine fever virus (CSFV) nucleic acid in archival formalin fixed tissue samples and their use for phylogenetic analysis was investigated. Ten samples were examined for the presence of CSFV nucleic acid by reverse transcriptase polymerase chain reaction (RT-PCR) amplification of 5 0 UTR and E2 gene. RT-PCR was found positive for 5 0 UTR fragment in eight samples while only one tissue samples showed amplification for E2 gene target fragment. For molecular epidemiology of the disease, 5 0 UTR PCR product of sample from Darbhanga (Bihar), was cloned and sequenced. The sequence was compared with the sequences available in database. The phylogenetic analysis reveals that the isolate belongs to subgroup 2.2 sharing 98.7% nucleotide identities with Vietnamese isolate (CaTh05-1, AB252170), indicating towards the possible origin of genogroup 2.2 CSFV isolates involved in the outbreak from Vietnam. From the study, it can be concluded that the tissue samples collected and stored in buffer formalin for years can be used to detect CSFV nucleic acid. Results are also suggestive of that the 5 0 UTR region of genome is more suitable target for RT-PCR based detection of CSFV in archival formalin fixed specimens. The study also indicates the potential of archival formalin fixed tissues for molecular epidemiology and genotyping of the CSF virus.

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Detection of classical swine fever virus in archival formalin-fixed tissues by reverse transcription-polymerase chain reaction

Cited by 8 publications

References 9 publications

An Outbreak of Classical Swine Fever in Indigenous Pigs in Tamilnadu, India

An Outbreak of Classical Swine Fever in Indigenous Pigs in Tamilnadu, India

Standardization and Validation of Qualitative Real Time Rt-PCR for Detection of Classical Swine Fever Virus

Phylogenetic analysis of Classical swine fever virus from archival formalin fixed clinical tissues reveals vietnamese origin of the isolates

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