Abstract:CTCs can be successfully isolated in patients with advanced-stage HNSCC using the CellSearch system. CTC detection may be important for prognosis, evaluating treatment outcome, and for determining efficacy of adjuvant treatments.
“…Data about CTC detection with the CellSearch system in patients with head and neck cancer have already been published; however, patient cohorts were heterogeneous with SCCs of different subanatomical regions, including hypo-and oropharyngeal cancer (29,30). Interestingly, absence or disappearance of CTCs during therapy was shown to be associated with partial or complete response to therapy (29).…”
Purpose: Current staging methods for squamous cell carcinomas (SCC) of the oral cavity (OSCC) need to be improved to predict the risk of individual patients. Because hematogenous tumor cell dissemination is a key event in tumor progression, we assessed the prognostic significance of disseminated tumor cells (DTC) in bone marrow and circulating tumor cells (CTC) in peripheral blood from patients with OSCC.Experimental Design: From 110 patients with OSCC, tumors were surgically resected (R0) without neoadjuvant therapy. The CellSearch system was used to enumerate CTCs. Bone marrow was aspirated from the iliac crest, and mononuclear cells (MNC) were enriched by Ficoll density gradient centrifugation. To detect DTCs, MNCs were immunostained with the pan-keratin antibody A45-B/B3. Results were correlated with clinicopathologic parameters and clinical outcome such as recurrence and death during follow-up time (mean 916 days).Results: Ten of 80 patients (12.5%) harbored CTCs in peripheral blood, whereas in 18 of 90 patients (20.0%) DTCs in bone marrow could be detected. Surprisingly, in only 2 patients (1.8%) CTCs and DTCs were detected simultaneously. Significant correlations could be found for CTCs and tumor size (P ¼ 0.04), nodal status and DTCs (P ¼ 0.02), and distant metastasis with CTCs (P ¼ 0.004) and DTCs (P ¼ 0.005). Univariate and multivariate analyses revealed that CTCs and DTCs were significant and independent predictors of recurrence-free survival (P < 0.001).Conclusions: Both DTCs and CTCs are independent prognostic markers in patients with OSCC, predicting relapse with higher sensitivity at various disease stages than routine staging procedures. Bone marrow might be an interesting target organ for future therapeutic interventions. Clin Cancer Res; 20(2); 425-33. Ó2013 AACR.
“…Data about CTC detection with the CellSearch system in patients with head and neck cancer have already been published; however, patient cohorts were heterogeneous with SCCs of different subanatomical regions, including hypo-and oropharyngeal cancer (29,30). Interestingly, absence or disappearance of CTCs during therapy was shown to be associated with partial or complete response to therapy (29).…”
Purpose: Current staging methods for squamous cell carcinomas (SCC) of the oral cavity (OSCC) need to be improved to predict the risk of individual patients. Because hematogenous tumor cell dissemination is a key event in tumor progression, we assessed the prognostic significance of disseminated tumor cells (DTC) in bone marrow and circulating tumor cells (CTC) in peripheral blood from patients with OSCC.Experimental Design: From 110 patients with OSCC, tumors were surgically resected (R0) without neoadjuvant therapy. The CellSearch system was used to enumerate CTCs. Bone marrow was aspirated from the iliac crest, and mononuclear cells (MNC) were enriched by Ficoll density gradient centrifugation. To detect DTCs, MNCs were immunostained with the pan-keratin antibody A45-B/B3. Results were correlated with clinicopathologic parameters and clinical outcome such as recurrence and death during follow-up time (mean 916 days).Results: Ten of 80 patients (12.5%) harbored CTCs in peripheral blood, whereas in 18 of 90 patients (20.0%) DTCs in bone marrow could be detected. Surprisingly, in only 2 patients (1.8%) CTCs and DTCs were detected simultaneously. Significant correlations could be found for CTCs and tumor size (P ¼ 0.04), nodal status and DTCs (P ¼ 0.02), and distant metastasis with CTCs (P ¼ 0.004) and DTCs (P ¼ 0.005). Univariate and multivariate analyses revealed that CTCs and DTCs were significant and independent predictors of recurrence-free survival (P < 0.001).Conclusions: Both DTCs and CTCs are independent prognostic markers in patients with OSCC, predicting relapse with higher sensitivity at various disease stages than routine staging procedures. Bone marrow might be an interesting target organ for future therapeutic interventions. Clin Cancer Res; 20(2); 425-33. Ó2013 AACR.
“…This hypothesis is strengthened by significantly higher incidences of CTC positivity in patients with locally advanced disease when considering the N-stage, holding true even in a multivariate analysis [31] . Moreover, CTC detection was significantly associated with the presence of lung nodules [84] . The tumour mass limits local resection by potentially decreasing safety margins because of neighbouring structures, such as the sinus at the skull base, which reaffirms the problem of an assumed CF.…”
Section: A Discussion Of the Results From Studies On Head And Neck Camentioning
confidence: 95%
“…Nevertheless, a longer disease-free survival of patients without detectable CTCs compared to CTC-positive patients was stated by Jatana et al [32] . Unfortunately, most of the studies cited were performed on small patient cohorts for other groups of HNSCC [83,84] . In a distinctly larger group of 176 HNSCC patients, the presence of DTCs in BM showed prognostic relevance for overall survival.…”
Section: A Discussion Of the Results From Studies On Head And Neck Camentioning
confidence: 99%
“…However, even in metastasised, advanced disease, CTCs were not detectable in all cases [35] . Nichols et al [84] detected almost 100% of spiked HNSCC cell line cells (FaDu) using the CellSearch system, however only 6 out of 15 HN-SCC patients tested CTC-positive using this approach. The results are in accordance with those of other studies but suggest that, in patients, either the incidence or number of potentially detectable CTCs is very low or that not all present CTCs are detectable with the currently applied approaches.…”
Section: A Discussion Of the Results From Studies On Head And Neck Camentioning
Due to a lack of substantial improvement in the outcome of patients suffering from oral squamous cell carcinoma (OSCC) during the past decades, current staging methods need to be revised. This disease is associated with poor survival rates despite considerable advances in diagnosis and treatment. The early detection of metastases is an important indicator of survival, prognosis and relapse. Therefore, a better understanding of the mechanisms underlying metastasis is crucial. Exploring alternative measures apart from common procedures is needed to identify new prognostic markers. Similar to previous findings predominantly for other solid tumours, recently published studies demonstrate that circulating tumour cells (CTCs) and disseminated tumour cells (DTCs) might serve as prognostic markers and could supplement routine staging in OSCC. Thus, the detection of CTCs/DTCs is a promising tool to determine the individual need for therapeutic intervention. Encouraging results and new approaches point to the future use of targeted therapies for OSCC, an exceedingly heterogeneous subgroup of head and neck cancer. This review focuses on summarising technologies currently used to detect CTCs/DTCs. The translational relevance for OSCC is highlighted. The inherent challenges in detecting CTCs/DTCs will be emphasised. Core tip: Oral squamous cell carcinoma (OSCC), among head and neck cancer, is related to poor survival rates despite considerable advances in diagnosis and treatment. Therefore, detecting tumour cell dissemination early and understanding the underlying mechanisms are crucial for predicting prognosis, relapse and survival. According to previous findings, circulating tumour cells (CTCs) and disseminated tumour cells (DTCs) might serve as prognostic markers to supplement routine staging and support determining individual therapeutic interventions. This review focuses on summarising the current knowledge about the detection of CTCs/DTCs with special emphasis on patients suffering from OSCC. The translational relevance of CTCs/DTCs and challenges for clinical application are highlighted.Wikner J, Gröbe A, Pantel K, Riethdorf S. Squamous cell carcinoma of the oral cavity and circulating tumour cells.
“…However, CTCs are promising as a prognostic factor in patients with advanced breast, colorectal, and prostate cancer and other tumor types, such as lung cancer, 45,46 melanoma, 47 head and neck cancer, 48 and ovarian cancer. 49 Even in the early stage of certain cancers without clinical or imaging signs of overt metastases, CTCs have significant prognostic relevance, particularly in breast cancer 50 but also in other cancer types.…”
Circulating tumor cells are low-frequency cells that are shed into the peripheral bloodstream from a primary solid tumor and/or metastasis. Although these cells were recognized initially in 1869, it is only in the past 2 decades that they have been isolated for use as a surrogate biomarker to monitor response to therapy, evaluate prognosis, detect tumor mutations, assist in selecting personalized medicine, and enable earlier cancer diagnosis.
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