Detection of circulating tumor cells using targeted surface-enhanced Raman scattering nanoparticles and magnetic enrichment

Shi, Wei; Paproski, Robert J.; Moore, Ronald B.; Zemp, Roger J.

doi:10.1117/1.jbo.19.5.056014

Cited by 47 publications

(34 citation statements)

References 23 publications

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“…Sun et al 138 synthesized biotin-labelled aptamers specific to DLD-1 cells (a colorectal adenocarcinoma cell line) and subsequently conjugated them to magnetic beads via the biotin-streptavidin interaction to develop a capture system for CTCs, which identified CTCs readily and accurately with a SERS imaging technique. Shi et al 139 used magnetic scattering nanoparticles (MNPs) decorated with folate (its receptor is overexpressed in many cancer cells) to target cancer cells and found that an increasing SERS signal was detected from the cells targeted by both SERS NPs and MNPs; this system was able to effectively trap cells within a large flow velocity range (0.2 to 12 cm s −1 ).…”

Section: Bloodmentioning

confidence: 99%

Biomedical optical spectroscopy for the early diagnosis of gastrointestinal neoplasms

2017

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Gastrointestinal cancer is a leading contributor to cancer-related morbidity and mortality worldwide. Early diagnosis currently plays a key role in the prognosis of patients with gastrointestinal cancer. Despite the advances in endoscopy over the last decades, missing lesions, undersampling and incorrect sampling in biopsies, as well as invasion still result in a poor diagnostic rate of early gastrointestinal cancers. Accordingly, there is a pressing need to develop noninvasive methods for the early detection of gastrointestinal cancers. Biomedical optical spectroscopy, including infrared spectroscopy, Raman spectroscopy, diffuse scattering spectroscopy and autofluorescence, is capable of providing structural and chemical information about biological specimens with the advantages of non-destruction, non-invasion and reagent-free and waste-free analysis and has thus been widely investigated for the diagnosis of oesophageal, gastric and colorectal cancers. This review will introduce the advances of biomedical optical spectroscopy techniques, highlight their applications for the early detection of gastrointestinal cancers and discuss their limitations.

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Section: Bloodmentioning

confidence: 99%

Biomedical optical spectroscopy for the early diagnosis of gastrointestinal neoplasms

2017

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“…As the Raman-scattered light is very weak, surface-enhanced Raman scattering (SERS) and stimulated Raman scattering (SRS) have been used to intensify the signals [7]. Raman-scattered light is also utilized in the detection of tumor cells [8,9], but use in the enclosed space of micro uidic devices is dif cult.…”

Section: Introductionmentioning

confidence: 99%

Immobilized Monolayer Nanoparticles in a Microfluidic Device for Surface Enhanced Raman Scattering Measurement

Hase

Ishigaki

Shibusawa

et al. 2017

ABE

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Micro uidic devices are powerful bioapplication tools for cellular experiments in particular, as they can regulate physical and chemical parameters such as the ow rate, shear stress, oxygen, and molecular concentrations in a culture medium to mimic physiological and pathological microenvironments in vitro. However, as cell cultures take place in enclosed spaces, culture samples have to be removed repeatedly to monitor cell-derived metabolites and proteins in order to maintain the cellular microenvironment. We report a simple method for obtaining surface enhanced Raman scattering (SERS) spectra through self-assembly of a nanoparticle monolayer on polydimethylsiloxane (PDMS), which is commonly used in highly biocompatible micro uidic devices. Silica nanoparticles were stabilized as a hexagonal close packed structure on an O 2 plasma-processed PDMS membrane, and was coated with silver using vapor deposition to create an SERS plate. When this SERS plate was installed in a micro uidic device, the nanoparticles did not peel off even after long-term uid immersion. The SERS spectra exhibited stable SERS generation and an enhancement factor of more than 1.5 × 10 6 of the Raman signals from rhodamine 6G compared to the signals without nanoparticles. The SERS spectra of lactate and ATP were obtained, and Raman shifts due to the different masses of 12 C-and 13 C-lactate were observed, suggesting that the proposed method can be applied to determine the cellular metabolic ux in micro uidic devices. We also obtained cell membrane-derived SERS spectra by culturing murine mammary carcinoma 4T1 cells directly on the nanoparticle membrane. PDMS has high biocompatibility and is often used for fabricating micro uidic devices. Through tight binding of the nanoparticles to the O 2 plasma-treated PDMS, the chemical reaction products or the metabolites from cells can be measured for a long duration, while maintaining the microenvironment. We anticipate that this technology can be utilized as a useful tool for analyzing cellular metabolic functions and imaging cellular distributions without staining in various pathological models created in micro uidic devices.

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“…17 Recently, we demonstrated SERS-based detection of magnetically trapped CTCs in vitro. 13 Here we extend this work to include a multimodality photoacoustic-SERS imaging platform and to demonstrate ultralow-background Raman-detection of SERS-targeted tumor cells in vivo. The PAM subsystem enables detailed visualization of the microvascular structure and potentially can guide the placement of a trapping magnet.…”

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confidence: 99%

“…[1][2][3][4][5][6][7][8][9][10][11][12][13] Fluorescence-based detection suffers from significant background autofluorescence.…”

mentioning

confidence: 99%

“…The SERS subsystem is capable of detecting single tumor cells if they are targeted with more than 200 SERS nanoparticles per cell. 13 To target widely overexpressed folate receptors on cancer cells, we conjugated both SERS nanoparticles (S440 & S421, BD Technologies) and magnetic fluorescent silica particles (screenMAG-Thiol, chemicell GmbH) with folate. 13 Simply, folate-PEG5k-maleimide (0.8 mM, PG2-FAML-5k, Nanocs Inc.) and methoxy-PEG2k-maleimide (4 mM, Sigma Aldrich) were added to thiolated SERS nanoparticle solution (1.3 nM, a 60 nm diameter Au core, and a 30 nm silica shell).…”

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confidence: 99%

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Multimodality Raman and photoacoustic imaging of surface-enhanced-Raman-scattering-targeted tumor cells

et al. 2016

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Detection of circulating tumor cells using targeted surface-enhanced Raman scattering nanoparticles and magnetic enrichment

Cited by 47 publications

References 23 publications

Biomedical optical spectroscopy for the early diagnosis of gastrointestinal neoplasms

Biomedical optical spectroscopy for the early diagnosis of gastrointestinal neoplasms

Immobilized Monolayer Nanoparticles in a Microfluidic Device for Surface Enhanced Raman Scattering Measurement

Multimodality Raman and photoacoustic imaging of surface-enhanced-Raman-scattering-targeted tumor cells

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