Detection of Chlamydia (Bedsonia) in Certain Infections of Man. I. Laboratory Procedures: Comparison of Yolk Sac and Cell Culture for Detection and Isolation

Use of Gentamicin in the Isolation of Subgroup A Chlamydia

“…Therefore, the identification of iodine-stained inclusions in irradiated McCoy cells provides a relatively simple and convenient method for isolation of these agents. This technique is more sensitive than yolk sac inoculation and much more suitable for the handling of large numbers of specimens (5). However, the control of bacterial and fungal contamination is especially difficult with specimens from the genital tract (B.…”

“…B. Wentworth et al, Health Lab. Sci., in press), and the streptomycin-vancomycin recommended by Gordon et al (5) does not always control the growth of organisms which interfere with examination of specimens for chlamydiae. Therefore, the use of gentamicin for the control of contamination in the irradiated McCoy cell system was evaluated.…”

Diagnosis of Chlamydia trachomatis Infection

“…Various formulations of transport media have been used [39][40][41] as it may improve yield. This is because it typically contains fetal bovine serum to improve viability as well as antimicrobials, to suppress growth by other organisms.…”

“…This is because it typically contains fetal bovine serum to improve viability as well as antimicrobials, to suppress growth by other organisms. The formulation used is similar to transport media used for rickettsiae, but not viral transport media, as this typically contains penicillin [39,40,42].…”

Isolation in McCoy cells ofChlamydia Psittaciobtained from the domestic duck(anas platyrhyncos)

“…Complete 2SP transport medium (Gordon et al, 1969) containing gentamicin sulphate (0.1 g I' 1 ), streptomycin sulphate (0.1 g I" 1 ), and kanamycin sulphate (0.1 g I" 1 ) was used for transporting and storing samples taken in the field.…”