Detection of catecholamines and metanephrines by radio-immunoassay in canine plasma

Francis, Roland C. E.; Pickerodt, Philipp A.; Salewski, Lothar; Boemke, Willehad; Höhne, Claudia

doi:10.1016/j.tvjl.2008.10.008

Cited by 10 publications

(7 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, cardiac contractility is increased, and the overall effects are increased HR and stroke volume. 6 The parasympathetic effects on the heart are mediated by the neurotransmitter acetylcholine, which activates muscarinic cholinergic receptors. Parasympathetic activation efficiently reduces cardiac pacemaker frequency and cell-to-cell conduction velocity and increases the refractory period, thereby decreasing HRs.…”

Section: Introductionmentioning

confidence: 99%

Scaling Relationships Among Heart Rate, Electrocardiography Parameters, and Body Weight

Aleixo

Alfonso

Oba

et al. 2017

Topics in Companion Animal Medicine

View full text Add to dashboard Cite

Although heart rate (HR) is one of the most important clinical parameters determined via physical examinations, little information is available on the normal HR in dogs, which may be related to the high variability of body weight (BW) in this species. HR is determined by the discharge rate of the sinus node, which is dependent on the autonomic nervous system and the release of catecholamines. The allometric relationship between BW and HR in different species has been described as inversely proportional; however, this relationship has been refuted. Certain authors have reported that the relationship between HR and BW in dogs is based on temperament as well as sympathetic autonomic stimulation of the sinus node in small breeds compared with large breeds. The aim of this study was to analyze the effects of weight, sex, age and temperament on the HR, heart rate variability and serum catecholamine (epinephrine and norepinephrine) levels in dogs. We evaluated 48 adult dogs of both sexes and various breeds and ages and divided the dogs into 5 BW groups: <5kg (n = 8), 5-10kg (n = 10), 10-25kg (n = 10), 25-45kg (n = 10), and >45kg (n = 10). The measured parameters were HR, breath rate (BR) and body temperature. We also performed an ambulatory electrocardiogram and electrocardiography (ECG) test for 24 hours (Holter monitor) and determined the serum levels of the catecholamines epinephrine and norepinephrine. We observed correlations between HR and sex; differences among the weight groups with respect to ECG variables and epinephrine levels; and differences among the temperament categories for certain clinical parameters, such as HR and BR. Age affected the R wave amplitude, and an allometric relationship was not observed between HR and BW in the dogs. Our results indicated that weight was associated with variations in the ECG variables; age and sex were associated with variations in HR; and temperament had a significant influence on the HR and BR of the dogs.

show abstract

Section: Introductionmentioning

confidence: 99%

Scaling Relationships Among Heart Rate, Electrocardiography Parameters, and Body Weight

Aleixo

Alfonso

Oba

et al. 2017

Topics in Companion Animal Medicine

View full text Add to dashboard Cite

show abstract

“…As a consequence, numerous methods have been reported for the quantification of biogenic monoamines and their respective metabolites, including colorimetric and fluorometric methods (Udenfriend et al , ; Hardeman et al , ), radioimmunoassays (Engbaek and Voldby, ; Francis et al , ) or enzyme‐linked‐immunosorbent assays (Westermann et al , ; Park and Paeng, ). Owing to major advances in chromatography‐based methods and column technologies, multianalyte detection capability has been greatly enhanced (Bosse et al , ) and high‐performance liquid chromatography (HPLC) is now widely used for separation of such coumpounds.…”

Section: Introductionmentioning

confidence: 99%

An HPLC‐ECD method for monoamines and metabolites quantification in cuttlefish (cephalopod) brain tissue

Bidel

Corvaisier

Jozet‐Alves

et al. 2016

Biomedical Chromatography

View full text Add to dashboard Cite

The cuttlefish belongs to the mollusk class Cephalopoda, considered as the most advanced marine invertebrates and thus widely used as models to study the biology of complex behaviors and cognition, as well as their related neurochemical mechanisms. Surprisingly, methods to quantify the biogenic monoamines and their metabolites in cuttlefish brain remain sparse and measure a limited number of analytes. This work aims to validate an HPLC-ECD method for the simultaneous quantification of dopamine, serotonin, norepinephrine and their main metabolites in cuttlefish brain. In comparison and in order to develop a method suitable to answer both ecological and biomedical questions, the validation was also carried out on a phylogenetically remote species: mouse (mammals). The method was shown to be accurate, precise, selective, repeatable and sensitive over a wide range of concentrations for 5-hydroxyindole-3-acetic acid, serotonin, dopamine, 3,4-dihydroxyphenylacetic acid and norepinephrine in the both extracts of cuttlefish and mouse brain, though with low precision and recovery for 4-hydroxy-3-methoxyphenylethylene glycol. Homovanillic acid, accurately studied in rodents, was not detectable in the brain of cuttlefish. Overall, we described here the first fully validated HPLC method for the routine measurement of both monoamines and metabolites in cuttlefish brain. Copyright © 2016 John Wiley & Sons, Ltd.

show abstract

“…Measurement of metanephrines rarely is performed in dogs. Urinary NMN‐to‐creatinine ratio has been shown to be significantly increased in dogs with pheochromocytoma compared with dogs with hyperadrenocorticism and healthy dogs, but plasma‐free metanephrines previously have only been measured in healthy laboratory Beagles . This study aimed to measure plasma fMN and fNMN concentrations in dogs with pheochromocytoma, adrenocortical tumors, nonadrenal disease, and healthy dogs by liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) and to determine the sensitivity and specificity of plasma fMN and fNMN concentration for the diagnosis of pheochromocytoma in dogs.…”

mentioning

confidence: 99%

Plasma‐Free Metanephrine and Free Normetanephrine Measurement for the Diagnosis of Pheochromocytoma in Dogs

Gostelow

Bridger

Syme

2013

Veterinary Internal Medicne

View full text Add to dashboard Cite

Background: Measurement of plasma-free metanephrines is the test of choice to identify pheochromocytoma in human patients.Objectives: To establish the sensitivity and specificity of plasma-free metanephrine (fMN) and free normetanephrine (fNMN) concentrations to diagnose pheochromocytoma in dogs.Animals: Forty-five client-owned dogs (8 dogs with pheochromocytoma, 11 dogs with adrenocortical tumors, 15 dogs with nonadrenal disease, and 11 healthy dogs.)Methods: A prospective study. EDTA plasma was collected from diseased and healthy dogs and submitted for fMN and fNMN measurement by liquid chromatography-tandem mass spectrometry (LC-MS/MS).Results .01] nmol/L); P < 0.01). When used to diagnose pheochromocytoma, a fMN concentration of 4.18 nmol/L had a sensitivity of 62.5% and specificity of 97.3%, and a fNMN concentration of 5.52 nmol/L had a sensitivity of 100% and specificity of 97.6%.Conclusions and Clinical Importance: Plasma fNMN concentration has excellent sensitivity and specificity for the diagnosis of pheochromocytoma in dogs, whereas fMN concentration has moderate sensitivity and excellent specificity. Measurement of plasma-free metanephrines provides an effective, noninvasive, means of identifying dogs with pheochromocytoma.

show abstract

Detection of catecholamines and metanephrines by radio-immunoassay in canine plasma

Cited by 10 publications

References 8 publications

Scaling Relationships Among Heart Rate, Electrocardiography Parameters, and Body Weight

Scaling Relationships Among Heart Rate, Electrocardiography Parameters, and Body Weight

An HPLC‐ECD method for monoamines and metabolites quantification in cuttlefish (cephalopod) brain tissue

Plasma‐Free Metanephrine and Free Normetanephrine Measurement for the Diagnosis of Pheochromocytoma in Dogs

Contact Info

Product

Resources

About