Detection of carcinogen–DNA adducts by radioimmunoassay

Chemical Carcinogenesis: Risk Factors, Early Detection and Biomedical Engineering

“…Determining the structure of DNA, DNA sequencing, and the PCR revolutionized cell biology, including carcinogenesis. Advances in detection of DNA damage, including postlabeling of DNA (Randerath et al, 1981), immunoassays (Poirier et al, 1977) and mass spectrometry as earlier discussed (Singh and Farmer, 2006) have allowed the detection of a single altered base in 10 9 nucleotides using human nuclear DNA. This technology can be extended to analyze DNA or RNA in a single cell (Klein, 2005).…”

“…Many technological advances have allowed conceptual ideas to be experimentally tested, including the sensitive detection of chemical carcinogens by high-pressure liquid chromatography (Esaka, et al, 2003) and mass spectrometry (Sigh and Farmer,2006), detection of DNA adducts by postlabeling (Randerath et al, 1981) and by specific antibodies (Poirier et al, 1977), transcriptional profiling by arrays ( Kallioniemi et al, 1992, Schena et al, 1995, and quantitation of mutagenicity of carcinogens using bacterial genetics (Ames et al, 1973 Advanced spectrophotometry -DNA microarray systems The latter is particularly promising as it enables the simultaneous measurement of transcription of thousands of genes using microchips containing thousands of probes of complementary DNA (cDNA) immobilized in predetermined array. But suffers the caveat of being very expensive especially for the developing countries that appear to be mostly in need of it currently.…”

The Scientific Basis for Carcinogen Detection and Primary Cancer Prevention

“…[29][30][31][32][33][34][35][36][37][38] Conclusion Although the science of carcinogenesis is making rapid progress in terms of increas ing our understanding of basic mecha nisms, and although exciting new short term tests and experimental animal models are being developed, we are approaching a crisis in the area of risk extrapolation. I believe that this is a soluble problem but will require specific new types of infor mation and research.…”

Electron microscopic visualization of N-acetoxy-N-2-acetylaminofluorene binding sites in ColE1 DNA by means of specific antibodies.

“…On the other hand, guanosine 5'-monophosphate, N-hydroxy-N-2-acetylaminofluorene, denatured DNA, and native DNA are not recognized (2). Guo-C8-AAF coupled with bovine serum albumin has also been shown to trigger the synthesis of specific antibodies in rabbits (3,4). Such antibodies are a specific tool enabling the titration of carcinogen binding sites in DNA by radioimmunoassay (2,5).…”