Detection of carbohydrate-binding proteins by oligosaccharide-modified polypyrrole interfaces using electrochemical surface plasmon resonance

Abstract

This paper reports on the use of electrochemical surface plasmon resonance (E-SPR) for the detection of carbohydrate-binding proteins. The generation of an SPR sensor specific to lectins Arachis hypogaea (PNA) and Maackia amurensis (MAA) is based on the electrochemical polymerization of oligosaccharide derivatives functionalized by pyrrole groups. The resulting thin conducting polymer films were characterized using E-SPR and atomic force microscopy (AFM). The specific binding of PNA to polypyrrole-lactosyl and of MAA to polypyrrole-3'-sialyllactosyl films was investigated using SPR. The detection limit was 41 nM for PNA and 83 nM for MAA. Through Scatchard analysis and linear transformation of the SPR sensorgram data, association (k(ass)) and dissociation rate constants (k(diss)) could be determined.

Cyclodextrin-based rotaxanes

“…Their ability to inhibit the binding of Arachis hypogaea agglutinin to asialofetuin was assessed by ELLA (enzyme linked lectin assays). Lactose was chosen for experiments due to its affinity for various galactose binding proteins such as peanut lectin [82].…”

Biosensors Based on Electropolymerized Films

“…Following the pioneering work of Cosnier et al [31], several recent reports have focused on the binding of oligosaccharides [32][33][34][35][36]. In particular, the specific binding of lectins such as Arachis hypogaea and Maackia amurensis to polypyrrole-lactosyl and polypyrrole-3 -sialyllactosyl films was nicely demonstrated by electrochemical SPR providing specific detection limits at the nanomolar level [34].…”

“…In particular, the specific binding of lectins such as Arachis hypogaea and Maackia amurensis to polypyrrole-lactosyl and polypyrrole-3 -sialyllactosyl films was nicely demonstrated by electrochemical SPR providing specific detection limits at the nanomolar level [34]. In this vein, the electropolymerization of natural oligosaccharides (molecular weight ranging from 3 to 45 kDa) was carried out for elaborating an SPR chip array.…”

Electrogenerated poly(pyrrole-lactosyl) and poly(pyrrole-3'-sialyllactosyl) interfaces: toward the impedimetric detection of lectins

“…Lectin detection has been investigated using different methods such as QCM (Shen et al, 2007; Wilczewski et al, 2008; Norberg et al, 2011, 2012), fluorescence (Kikkeri et al, 2009; Sanji et al, 2009; Shiraishi et al, 2010; Mandal et al, 2012), SPR (Vornholt et al, 2007; Gondran et al, 2008; Murthy et al, 2008; Wilczewski et al, 2008; Spain and Cameron, 2011), voltammetry (Casas-Solvas et al, 2008; Hu et al, 2012), electrochemical impedance spectroscopy (EIS) (Dubois et al, 2005; Szunerits et al, 2010; Xi et al, 2011; Oliveira et al, 2011). EIS is an efficient and sensitive technique suitable for the characterization of deposits on electrode surfaces and their evolution.…”

“…We, previously, reported the synthesis of pyrrole-functionalized oligosaccharides: pyrrole-lactosyl and pyrrole-3′-sialyllactosyl and their use for the generation of SPR sensors specific to two lectins ( Arachis hypogaea , PNA and Maackia amurensis MAA agglutinins) (Gondran et al, 2008). In this context, we report here the electrochemical characterization of these carbohydrate-polymers and the investigations of their potentialities for the impedimetric detection of lectins and hence for the development of label-free impedance sensors (Figure 1).…”