Detection of canine oral papillomavirus-DNA in canine oral squamous cell carcinomas and p53 overexpressing skin papillomas of the dog using the polymerase chain reaction and non-radioactive in situ hybridization

Teifke, Jens Peter; Löhr, Christiane V.; Shirasawa, Hiroshi

doi:10.1016/s0378-1135(98)00151-5

Cited by 81 publications

(85 citation statements)

References 18 publications

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“…2,7 In this study, immunohistochemistry showed strong positivity in all tissues suspected to be associated with papillomavirus infection. The monoclonal antibodies against papillomavirus antigens used in this study differed from the polyclonal antibody used in both prior accounts, possibly affecting the difference observed between the results seen in our study compared with previous studies.…”

Section: Discussionsupporting

confidence: 53%

Squamous Papillomas of the Conjunctiva in Dogs

et al. 2014

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Papillomas of the conjunctival surface in people can be of viral or nonviral origin and are found in high association with human papillomavirus. Canine conjunctival papillomas are seldom described, and published accounts have mostly been associated with canine oral papillomavirus infection. Here, we describe conjunctival squamous papillomas that do not express papillomavirus proteins and compare them with papillomavirus-associated conjunctival papillomas. Conjunctival squamous papillomas presented a distinct histopathologic profile and lacked the cytopathic effects seen in viral papillomas. They appeared as exophytic, papilliferous, pedunculated lesions with delicate fronds and angular terminal margins. Squamous papillomas presented with a delicate fibrovascular core and were associated both clinically and grossly with a feeder vessel. Pigmentation was variable within the epithelium and stroma of these lesions, and inflammatory infiltrates were characteristically minimal. Conjunctival squamous papillomas resembled squamous papillomas of the skin; however, they lacked significant hyperkeratosis. Compared with conjunctival viral papillomas, these masses occurred in older dogs and were smaller and solitary. Furthermore, polymerase chain reaction and immunohistochemistry failed to demonstrate papillomavirus genetic material and antigens in conjunctival squamous papillomas. Both viral and nonviral conjunctival papillomas were considered benign.

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Section: Discussionsupporting

confidence: 53%

Squamous Papillomas of the Conjunctiva in Dogs

et al. 2014

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“…CPV1, originally denominated canine oral papillomavirus (COPV) (Bernard et al 2010), induces papillomas at the mucous membranes of the oral cavity and at the haired skin of dogs (Gross et al 2005); papillomas may progress to squamous cell carcinoma (Teifke et al 1998). Additionally, CPV1 has been associated with inverted papillomas (Lange et al 2009), and conjuctival epithelial hyperplastic lesions ) in dogs.…”

Section: Introductionmentioning

confidence: 99%

Identification of canine papillomavirus type 1 (CPV1) DNA in dogs with cutaneous papillomatosis

et al. 2014

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Canine oral papillomavirus (COPV), also known as Canine Papillomavirus type 1 (CPV1), induces papillomas at the mucous membranes of the oral cavity and at the haired skin of dogs. The classification of Papillomavirus (PV) types is based on the L1 capsid protein and nucleotide sequence; so far, 14 CPV types have been described in several countries, but the molecular characterization of CPV in Brazil is lacking. This study investigated the presence of the PV in seven papillomas from four mixed breed dogs from Londrina/PR, Southern Brazil, by partial sequencing of the L1 gene. Seven exophytic cutaneous lesions were surgically removed and processed for histopathological and molecular characterization. Histopathology confirmed the lesions as viral papillomas due to typical histological features. Polymerase Chain Reaction (PCR) assay using the FAP59 and FAP64 primers targeted the L1 gene followed by sequence analysis of the amplicons identified CPV1 in all evaluated papilloma samples. This study represents the first description of CPV1 DNA associated with canine papillomatosis in Brazil.

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“…COPV infects domestic dogs and wild canine species on the oropharyngeal and conjunctival mucosae where it induces warts (and very rarely squamous cell carcinomas (3,26,29)). The development of lesions is highly reproducible both in terms of wart area and time providing clean readouts for vaccine efficacy.…”

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confidence: 99%