Squamous Papillomas of the Conjunctiva in Dogs

Beckwith-Cohen, Billie; Teixeira, Leandro; Ramos‐Vara, José A.; Dubielzig, Richard R.

doi:10.1177/0300985814556185

Cited by 17 publications

(11 citation statements)

References 8 publications

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“…Papillomavirus has been detected in conjunctival plaques and viral papillomas, and an eyelid squamous cell carcinoma in dogs [23, 28, 39]. However, Beckwith-Cohen et al showed that papillomavirus was not present in 10 conjunctival squamous papillomas or normal conjunctival tissue from one dog [39]. Our current study is consistent with the Beckwith-Cohen study as papillomavirus was not present in normal conjunctival tissue or tissue from canine lobular orbital adenomas.…”

Section: Discussionsupporting

confidence: 89%

“…Several other studies have evaluated normal and abnormal canine ocular tissue for the presence of papillomavirus. Papillomavirus has been detected in conjunctival plaques and viral papillomas, and an eyelid squamous cell carcinoma in dogs [23, 28, 39]. However, Beckwith-Cohen et al showed that papillomavirus was not present in 10 conjunctival squamous papillomas or normal conjunctival tissue from one dog [39].…”

Section: Discussionmentioning

confidence: 99%

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Papillomavirus DNA not detected in canine lobular orbital adenoma and normal conjunctival tissue

et al. 2019

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Background Canine lobular orbital adenomas are benign tumors that arise from orbital glandular tissue and extend into the orbit, conjunctiva, and third eyelid. Surgical excision is challenging and recurrence rates are high following excision alone. Enucleation and exenteration reduces the likelihood of recurrence, but is a radical therapeutic option for an otherwise visual and comfortable eye. Human papillomavirus causes 4.5% of worldwide cancers in people and has been identified in up to 23% of benign salivary gland tumors. To date, the etiology of canine lobular orbital adenomas has not been established and it is reasonable to consider canine papillomaviruses as an associative agent with benign glandular tumors in dogs. Identification of the underlying etiology of these tumors may help establish treatment or preventative measures. The purpose of this study was to evaluate conjunctival and orbital tissue of phenotypically normal dogs and tissue from canine lobular orbital adenomas for the presence of papillomavirus DNA. Results Thirty seven canine lobular orbital adenoma samples (36 formalin fixed paraffin embedded (FFPE) tissue samples from 33 dogs and one freshly collected sample) were evaluated via polymerase chain reaction for the presence of papillomavirus DNA. Conjunctival tissue samples, from 10 dogs with normal ocular examinations, excised immediately following euthanasia, were used as phenotypically normal controls. Three FFPE and one freshly collected tissue samples previously confirmed to be positive for papillomavirus DNA were used as positive controls. PCR products verified positive controls. Papillomavirus DNA was not detected in fresh conjunctival tissue of the phenotypically normal control dogs or in samples of fresh or FFPE canine lobular orbital adenoma tissue. Conclusions An association between papillomavirus and the development of canine lobular orbital adenomas is unlikely. Further research is needed to evaluate if other viruses play a role in the pathogenesis of canine lobular orbital adenomas.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Papillomavirus DNA not detected in canine lobular orbital adenoma and normal conjunctival tissue

et al. 2019

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“…ab2417, Abcam, Cambridge, UK) were 100-fold diluted in PBST and applied to the slides for incubation for one hour at room temperature. This antibody broadly reacts to several types of CPV, BPV, HPV, and Zalophus papillomavirus [38][39][40][41][42]. The endogenous peroxidase activity was blocked by incubating the slides with 3% H2O2 in methanol for 10 min.…”

Section: Immunohistochemistry Staining Of Viral Antigensmentioning

confidence: 99%

The Detection and Association of Canine Papillomavirus with Benign and Malignant Skin Lesions in Dogs

Chang

Chen

Haga

et al. 2020

Viruses

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Papillomavirus (PV) mainly infects the squamous epithelium and may potentially lead to benign or even malignant cutaneous lesions. However, the malignant transforming ability has been identified in several types of PVs. In humans, papillomavirus (HPV) type 16 and 18 are the most prevalent causative agents of cervical cancer. Therefore, vaccines are being developed to protect against these types. For dogs, there have been limited investigations into the association of different canine papillomavirus (CPV) genotypes with malignant lesions. Understanding the high-risk CPV genotype(s) responsible for these malignant lesions would contribute to the development of interventions for preventing CPV-induced carcinomas. In the present study, a retrospective cohort of 102 pathologically confirmed papillomas and 212 squamous cell carcinomas (SCCs) were included. The viral genome and antigens in the formalin-fixed paraffin-embedded (FFPE) tissues were detected using PCR targeting pan PV E1 and COPV L1 genes and by immunohistochemistry staining (IHC), respectively. PVs were successfully detected from 11 FFPE cutaneous tissues and four oral tissues using pan PV E1- and COPV L1-based PCR, respectively. After sequencing, CPV 1, CPV 2, and CPV 6 were detected in the benign lesions using PCR and were confirmed through IHC. While CPV 9 and CPV 15 were first detected in the SCCs of dogs, CPV 16 was most often detected in SCC specimens. The association and confirmative demonstration of viral genes and intralesional antigens of CPV 9, CPV 15, and CPV 16 in SCCs highlight the potential risk of these genotypes of CPVs in malignant transformation.

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“…Moreover, recent publications suggested that, squamous papilloma of skin and conjunctiva in dogs are not connected with papillomaviruses. 7 …”

Section: Introductionmentioning

confidence: 99%

Electrochemotherapy with bleomycin of different types of cutaneous tumours in a ferret (Mustela putorius furo)

Račnik

Švara

Zadravec

et al. 2017

Radiology and Oncology

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BackgroundMast cell tumour, sebaceous gland adenoma, and less common squamous papilloma are skin tumours in ferrets (Mustela putorius furo), and early excisional surgery is usually the treatment of choice. The aim of our study was to investigate the effectiveness of electrochemotherapy (ECT), a new, minimally invasive non-surgical method, as first treatment option of different types of ferret skin tumours located on surgically difficult sites.Materials and methodsA 5-year-old castrated male ferret with two cutaneous masses, presenting 4 months apart and a 7-year-old spayed female ferret with two cutaneous masses, that appeared simultaneously on two locations are presented. In the first patient, both masses were diagnosed as mast cell tumours, and in the second patient, squamous papilloma and sebaceous adenoma were diagnosed. One session of ECT with bleomycin injected intratumourally was applied in all tumours.ResultsComplete response (CR) of all tumours was obtained, without recurrence during observation period of 15 months after ECT for first tumour and 11 months after ECT of the tumour located on the right hock in first patient, and 8 months after treatment for the second patient.ConclusionsIn present study, ECT with bleomycin proved to be safe and effective against different cutaneous tumours in ferrets. Due of good results, low cost and relatively easy procedure, ECT could be the treatment of choice instead of surgery for the selected skin tumours in ferrets.

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Squamous Papillomas of the Conjunctiva in Dogs

Cited by 17 publications

References 8 publications

Papillomavirus DNA not detected in canine lobular orbital adenoma and normal conjunctival tissue

Papillomavirus DNA not detected in canine lobular orbital adenoma and normal conjunctival tissue

The Detection and Association of Canine Papillomavirus with Benign and Malignant Skin Lesions in Dogs

Electrochemotherapy with bleomycin of different types of cutaneous tumours in a ferret (Mustela putorius furo)

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