Detection of Canine Microalbuminuria Using Semiquantitative Test Strips Designed for Use with Human Urine

Pressler, Barrak M.; Vaden, Shelly L.; Jensen, Wayne A.; Simpson, Dan

doi:10.1111/j.1939-165x.2002.tb00280.x

Cited by 34 publications

(24 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Because albuminuria is the earliest detectable form of proteinuria and all dogs with CKD had a UPC > 0.5, the strong correlation between uALB/c and UPC was expected 28 . This strong correlation also was found in a study in cats with CKD, in which UPC also was predictive for survival 25 …”

Section: Discussionsupporting

confidence: 75%

“…The assays used in this study were able to measure canine uALB and uNAG in a linear manner with within‐ and between‐run imprecision of 5.2 and 12%, and 4.9 and 8.1%, respectively. As a comparison, for uALB ELISA's, other studies have reported within‐ and between‐run imprecision ranging between 2–17 and 4.5–21.7%, respectively 25,26 . For the NAG colorimetric assay, within‐ and between‐run CVs of 2.8 and 11.9% have been reported 27 .…”

Section: Discussionmentioning

confidence: 96%

See 1 more Smart Citation

Urinary Markers in Healthy Young and Aged Dogs and Dogs with Chronic Kidney Disease

Smets

Meyer

Maddens

et al. 2010

Veterinary Internal Medicne

101

View full text Add to dashboard Cite

Background: Blood urea nitrogen and serum creatinine concentrations only detect a decrease of 475% of renal functional mass. Therefore, there is a need for markers that allow early detection and localization of renal damage.Hypothesis: Urinary albumin (uALB), C-reactive protein (uCRP), retinol binding protein (uRBP), and N-acetyl-b-Dglucosaminidase (uNAG) concentrations are increased in dogs with chronic kidney disease (CKD) compared with healthy controls and in healthy older dogs compared with young dogs.Animals: Ten dogs with CKD, 10 healthy young dogs (age 1-3 years), and 10 healthy older dogs (age 4 7 years) without clinically relevant abnormalities on physical examination, hematology, biochemistry, and urinalysis.Methods: Urinary markers were determined using an ELISA (uALB, uCRP, and uRBP) or a colorimetric test (uNAG). Results were related to urinary creatinine (c). The fixed effects model or the Wilcoxon rank sum test were used to compare the different groups of dogs.Results: uALB/c, uRBP/c, and uNAG/c were significantly higher in CKD dogs than in healthy dogs. No significant difference was found for uCRP, which was not detectable in the healthy dogs and only in 3 of the CKD dogs. Between the healthy young and older dogs, no significant difference was detected for any of the markers.Conclusion: The urinary markers uALB/c, uRBP/c, and uNAG/c were significantly increased in dogs with CKD compared with healthy controls. Additional studies are needed to evaluate the ability of these markers to detect renal disease before the onset of azotemia.

show abstract

Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 96%

Urinary Markers in Healthy Young and Aged Dogs and Dogs with Chronic Kidney Disease

Smets

Meyer

Maddens

et al. 2010

Veterinary Internal Medicne

101

View full text Add to dashboard Cite

show abstract

“…10,17 To our knowledge, this technique has only been validated in dogs for urine albumin. 18 In a study that analyzed canine CSF by nephelometry, g only a few dogs showed an increased AQ associated with a normal value for CSF total protein. However, there were no dogs in our study with an AQ indicating a BBB impairment when the CSF total protein value was normal.…”

Section: Discussionmentioning

confidence: 99%

High Resolution Protein Electrophoresis of 100 Paired Canine Cerebrospinal Fluid and Serum

Behr

Trumel

Cauzinille

et al. 2006

Veterinary Internal Medicne

View full text Add to dashboard Cite

This study was performed to investigate the diagnostic relevance of cerebrospinal fluid (CSF) high resolution electrophoresis. The laboratory technique was applied to 100 paired samples of canine CSF and serum, with paired samples tested during the same analytical run, as recommended in human medicine. Ninety four of the dogs had a neurological disease and 6 healthy dogs served as a control group. A strong linear correlation between CSF total protein concentration and the albumin quota (AQ) was found in the control group and in the inflammatory (infectious or noninfectious), neoplastic, and miscellaneous groups: AQ 5 0.015 CSF total protein 2 0.102, r 5 0.990. This correlation suggests that an increased CSF total protein concentration can be an indicator of blood brain barrier dysfunction. The highest median AQ value was found in the aseptic suppurative meningitis group, but no statistical differences were found between this and the other groups. The AQ, calculated with this technique, did not provide any additional information. Moreover, although unexpected, the electrophoretic profiles were not characteristic of any particular disease. In conclusion, this study did not confirm high resolution electrophoresis of paired CSF and serum samples to be a valuable ancillary diagnostic tool for canine neurological diseases.

show abstract

“…[1][2][3][4] Semiquantitative point-of-care tests have been used for the detection of microalbuminuria in people for several years, but these same tests are not valid for use in the dog. 5 Recently, an in-clinic test for canine microalbuminuria was validated and marketed for use ( as urine albumin concentrations Ͼ1 mg/dL and Ͻ30 mg/ dL (ie, concentrations that are at least 2 standard deviations above the mean urinary albumin concentration in healthy dogs but that are undetectable by routinely employed urine dipstick assays). Several conditions are known to induce a short-lived, reversible, and clinically insignificant proteinuria, 6 but the effect of these conditions on albuminuria in dogs is largely unexplored.…”

mentioning

confidence: 99%

The Effects of Exercise on Urinary Albumin Excretion in Dogs

Gary¹,

Cohn²,

Kerl³

et al. 2004

J Vet Int Med

Self Cite

View full text Add to dashboard Cite

Persistent microalbuminuria has been shown to be an indicator of glomerular damage associated with early progressive renal disease in people and dogs. In people, transient or reversible microalbuminuria has been shown to occur with exercise. A semi-quantitative test to measure microalbuminuria in the dog recently has become available. The purpose of this study was to determine if mild-to-moderate exercise induced microalbuminuria in the dog. Twenty-six dogs were included in the study after undergoing tests to rule out hyperglycemia, urinary tract infection, azotemia, and a urine protein:creatinine ratio >1. Exercise consisted of 20 minutes of flat treadmill running. Urine samples were collected on 2 separate days before exercise, the morning of exercise, 3 hours postexercise, 7-9 hours postexercise, and each of the 2 mornings after exercise. For 24 of 26 dogs, this procedure was repeated after a minimum 7-day interval between exercise sessions. The canine E.R.D. (early renal disease)-Screen Urine Test (E.R.D.-Screen test) was used to determine semiquantitative urine albumin concentrations. Microalbuminuria-positive samples, as determined by the E.R.D.-Screen test, were further analyzed to determine quantitative albumin concentrations. Four (15%) dogs were microalbuminuria positive. In each of these dogs, microalbuminuria was present both before and after exercise with no quantitative increase in urine albumin concentration postexercise. Twenty-two (85%) dogs were microalbuminuria negative throughout the study and did not develop microalbuminuria at any time after exercise. On a 95% confidence interval, the proportion of dogs that might be expected to develop microalbuminuria after exercise is between 0 and 15%.

show abstract

Detection of Canine Microalbuminuria Using Semiquantitative Test Strips Designed for Use with Human Urine

Cited by 34 publications

References 7 publications

Urinary Markers in Healthy Young and Aged Dogs and Dogs with Chronic Kidney Disease

Urinary Markers in Healthy Young and Aged Dogs and Dogs with Chronic Kidney Disease

High Resolution Protein Electrophoresis of 100 Paired Canine Cerebrospinal Fluid and Serum

The Effects of Exercise on Urinary Albumin Excretion in Dogs

Contact Info

Product

Resources

About