High Resolution Protein Electrophoresis of 100 Paired Canine Cerebrospinal Fluid and Serum

Behr, Sébastien; Trumel, Cathy; Cauzinille, Laurent; Palenché, Florence; Braun, Jean‐Pierre

doi:10.1111/j.1939-1676.2006.tb02911.x

Cited by 19 publications

(5 citation statements)

References 16 publications

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“…In this study, an increased gamma globulin ratio and elevated albumin levels were observed in the CSF of all dogs with EAE. In addition, patterns of the CSF albumin and gamma globulin ratio increases were similar to that observed in cases of canine autoimmune encephalitis [ 2 ].…”

Section: Discussionsupporting

confidence: 62%

A study of experimental autoimmune encephalomyelitis in dogs as a disease model for canine necrotizing encephalitis

et al. 2015

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In the present study, the use of dogs with experimental autoimmune encephalomyelitis (EAE) as a disease model for necrotizing encephalitis (NE) was assessed. Twelve healthy dogs were included in this study. Canine forebrain tissues (8 g), including white and grey matter, were homogenized with 4 mL of phosphate-buffered saline for 5 min in an ice bath. The suspension was emulsified with the same volume of Freund's complete adjuvant containing 1 mg/mL of killed Mycobacterium tuberculosis H37Ra. Under sedation, each dog was injected subcutaneously with canine brain homogenate at four sites: two in the inguinal and two in the axillary regions. A second injection (booster) was administered to all the dogs using the same procedure 7 days after the first injection. Clinical assessment, magnetic resonance imaging, cerebrospinal fluid analyses, necropsies, and histopathological and immunohistochemical examinations were performed for the dogs with EAE. Out of the 12 animals, seven (58%) developed clinically manifest EAE at various times after immunization. Characteristics of canine EAE models were very similar to canine NE, suggesting that canine EAE can be a disease model for NE in dogs.

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Section: Discussionsupporting

confidence: 62%

A study of experimental autoimmune encephalomyelitis in dogs as a disease model for canine necrotizing encephalitis

et al. 2015

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“…Each dog presented a type 1 protein electrophoresis profile consistent with those previously described in healthy dogs (Behr et al, 2006) (Fig. 2).…”

Section: Csf Albumin Concentration and Qalbsupporting

confidence: 55%

“…Electrophoresis profiles with low albumin concentrations are frequently non-interpretable (Behr et al, 2006). Thanks to the microconcentration step we used, all electrophoresis profiles were interpretable even for albumin concentrations <20 mg/dL.…”

Section: Tablementioning

confidence: 99%

Evaluation of the effect of oral omeprazole on canine cerebrospinal fluid production: A pilot study

Girod

Allerton

Gommeren

et al. 2016

The Veterinary Journal

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A B S T R A C TAdministration of omeprazole by ventriculo-cisternal perfusion or intravenously has been shown to decrease cerebrospinal fluid (CSF) production in dogs and rabbits. Oral omeprazole has consequently been recommended to reduce CSF production in dogs with conditions in which clinical signs may be attributable to an accumulation of CSF in the central nervous system (e.g. hydrocephalus, syringomyelia). The albumin quotient (QAlb), the ratio between CSF and serum albumin concentration, has been proposed as a reliable means to evaluate CSF production; decreasing CSF production should cause an increase in QAlb.The aims of this study were to assess the effect of oral administration of omeprazole on QAlb in dogs and to compare two methods to assess CSF albumin concentration. Fifteen healthy Beagle dogs received omeprazole (1.2 mg/kg/day) orally for 14 days; CSF and blood were obtained before and after treatment. CSF albumin concentrations were evaluated by nephelometry and high-resolution protein electrophoresis. Regardless of the method used for measuring albumin, QAlb did not change significantly following oral omeprazole administration, suggesting that CSF production in healthy dogs may not be affected by chronic oral therapy with omeprazole.

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“…22 Recently, high-resolution electrophoresis (HRE) has been proposed as an electrophoretic technique to be applied to fluids with low concentration of proteins such as urine or cerebrospinal fluid. 2,3,8,9,12,21 HRE is a zonal electrophoresis in which proteins migrate based on charge, volume, and mass. HRE differs from the standard AGE (the technique routinely used in serum) by the use of a more alkaline buffer (pH 8.6 vs. 8.5), a different barbital content (0.18% vs. 0.61%), and a different voltage per hour (40 V/hr vs. 36 V/hr) at 20°C.…”

Section: Introductionmentioning

confidence: 99%

High-resolution gel electrophoresis and sodium dodecyl sulphate–agarose gel electrophoresis on urine samples for qualitative analysis of proteinuria in dogs

et al. 2011

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The aims of the current study were to assess whether sodium dodecyl sulphate-agarose gel electrophoresis (SDS-AGE) and high-resolution electrophoresis (HRE) can identify dogs with a urinary protein-to-creatinine ratio (UPC ratio) >0.2 and whether HRE can provide preliminary information about the type of proteinuria, using SDS-AGE as a reference method. HRE and SDS-AGE were conducted on 87 urine samples classified according to the International Renal Interest Society as non-proteinuric (NP; UPC ratio: <0.20; 32/87), borderline proteinuric (BP; UPC ratio: 0.21-0.50; 15/87), or proteinuric (P; UPC ratio: >0.51; 40/87). SDS-AGE and HRE were positive in 14 out of 32 and 3 out of 32 NP samples and in 52 out of 55 and 40 out of 55 samples with a UPC ratio >0.20, respectively. The concordance between HRE or SDS and UPC ratio was comparable (κ = 0.59; κ = 0.55). However, specificity (90%) and positive likelihood ratio (7.76) were higher for HRE than for SDS-AGE (56% and 2.16) while sensitivity was lower (73% vs. 94%). The analysis of HRE results revealed that a percentage of albumin >41.4% and an albumin/α(1)-globulin ratio (alb/α(1) ratio) >1.46 can identify samples classified by SDS-AGE as affected by glomerular proteinuria while a percentage of α(1)-globulin >40.8% and an alb/α(1) ratio <0.84 can identify samples classified by SDS-AGE as affected by tubular proteinuria. In conclusion, both SDS-AGE and HRE could misclassify samples with a UPC ratio higher or lower than 0.20. Therefore, UPC ratio must always be determined before conducting these tests. The percentage of albumin and α(1)-globulin or the alb/α(1) ratio determined by HRE can provide preliminary information about the origin of proteinuria.

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High Resolution Protein Electrophoresis of 100 Paired Canine Cerebrospinal Fluid and Serum

Cited by 19 publications

References 16 publications

A study of experimental autoimmune encephalomyelitis in dogs as a disease model for canine necrotizing encephalitis

A study of experimental autoimmune encephalomyelitis in dogs as a disease model for canine necrotizing encephalitis

Evaluation of the effect of oral omeprazole on canine cerebrospinal fluid production: A pilot study

High-resolution gel electrophoresis and sodium dodecyl sulphate–agarose gel electrophoresis on urine samples for qualitative analysis of proteinuria in dogs

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