Detection of canine distemper virus in stray and pet dogs in Mosul city, Iraq

Saaed, Muhanad M.; Alsarhan, Qaes T.

doi:10.33899/ijvs.2021.130127.1739

Cited by 2 publications

(2 citation statements)

References 16 publications

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“…A total of 442 examined smears comprising (185 thick blood smears and 185 thin blood smears) and (72 lymph smears) were prepared, air dried, then stained with MGG-Quick stain (Bio-Optic, Italy) and examined under a light microscope at (X1000) with immersion oil (Leitz, Germany) (21). For the initial investigation of Theileria spp.…”

Section: Microscopic Examination Of Blood and Lymph Smearsmentioning

confidence: 99%

Prevalence of ovine theileriosis in Mosul city, Iraq

Hamid¹,

Al-Obaidi²

2022

IJVS

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The present study aimed to determine the prevalence of ovine theileriosis (OT) in sheep in Mosul city, Iraq using microscopic examination (ME) of the blood smears stained with MGG-Quick stain and conventional polymerase chain reaction technique (c-PCR) to compare between c-PCR technique and ME as techniques for the diagnosis of disease, and to investigate the pattern and type of infections based on multiplex polymerase chain reaction technique (m-PCR). From October 2021 to May 2022, one-handed eighty-five Blood samples were drawn randomly from sheep in various regions of Mosul city. The overall prevalence of OT was 42% (22.7 out of 185) and 52.4% (97 out of 185) using microscopic examination and c-PCR technique, respectively. A slight agreement was observed between ME of blood smears and c-PCR technique according to Kappa value 0.190, with low sensitivity, specificity, and accuracy of ME method was 30%, 88.6%, 58.4%, respectively, compared with c-PCR technique. The prevalence of mixed infection 22.7% and single infection with T. lestoquardi 20% were significantly higher (P<0.05) than single infection with T. ovis 9.7%. This study concludes that OT is widespread in Mosul city, Iraq, and the c-PCR technique is more reliable and suitable for detecting Theileria infection in sheep than the ME method.

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Section: Microscopic Examination Of Blood and Lymph Smearsmentioning

confidence: 99%

Prevalence of ovine theileriosis in Mosul city, Iraq

Hamid¹,

Al-Obaidi²

2022

IJVS

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“…The test can detect distemper virus in the conjunctiva, urine, serum, or plasma with high accuracy. CDV antigen rapid test has shown excellent sensitivity (98%) and specificity (98.5%) compared to other methods (Costa et al, 2019;Saaed & Alsarhan, 2022). In our study, we used a Bionote (Woodley equipment) rapid test kit with a sensitivity of 100% and specificity of 98.5% compared to the nested PCR.…”

Section: Introductionmentioning

confidence: 99%

Molecular Detection of Canine Distemper Virus Among Dogs Showing Neurologic and Non-neurologic Forms of Disease

Mojtahedzadeh,

Jamshidi,

Ghalyanchi Langroudi

et al. 2024

IJVM

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Background: Canine distemper (CD) is the dogs’ most contagious and lethal viral disease. Despite the widespread use of vaccines to control CD, the prevalence of the CD virus (CDV) has increased at an alarming rate in recent years. Objectives: To identify the genotypes responsible for the neurological and non-neurological clinical forms of CD and to investigate the presence of the virus in the neurological and non-neurological forms of the disease. Methods: In this descriptive-analytical study, the samples were collected from 70 CD suspected unvaccinated dogs with clinical signs of distemper. All cases were tested with rapid tests and separated into 3 groups based on clinical signs and symptoms. Cerebral spinal fluid (CSF), respiratory secretion, and fecal samples of allall 70 cases were examined for reverse transcription polymerase chain reaction (RT-PCR). After sequencing the hemagglutinin gene (H gene), phylogenetic analysis of the gene isolated from CDVs was carried out using MEGA software, version 7. Results: The RT-PCR results showed that the respiratory secretion sample in the non-neurological CDV group (85%) and the neurological CDV group (80%) had the highest level of virus contamination. However, in the non-neurological CDV group, the CSF sample (40%) had a high level of infection. In neurotic groups, cases older than 12 months showed the highest percentage of distemper contamination, and in the non-neurologic CDV group, those between 3 and 6 months were more involved. Sequencing and phylogenetic analysis of the H gene revealed the CDV as a member of the endemic Arctic-like genetic lineage. Conclusion: The genotypic examination of the hemagglutinin gene of the distemper virus reveals that the recent isolates of neurologic and non-neurologic clinical forms of CDV in Iran are similar. In positive rapid test cases, the PCR test of respiratory secretions for virus detection ranks with the highest sensitivity. In neurologic cases with negative rapid test results, PCR of CSF had the highest sensitivity, so it may serve as a diagnostic criterion.

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Detection of canine distemper virus in stray and pet dogs in Mosul city, Iraq

Cited by 2 publications

References 16 publications

Prevalence of ovine theileriosis in Mosul city, Iraq

Prevalence of ovine theileriosis in Mosul city, Iraq

Molecular Detection of Canine Distemper Virus Among Dogs Showing Neurologic and Non-neurologic Forms of Disease

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