Prevalence of ovine theileriosis in Mosul city, Iraq

Abstract

The present study aimed to determine the prevalence of ovine theileriosis (OT) in sheep in Mosul city, Iraq using microscopic examination (ME) of the blood smears stained with MGG-Quick stain and conventional polymerase chain reaction technique (c-PCR) to compare between c-PCR technique and ME as techniques for the diagnosis of disease, and to investigate the pattern and type of infections based on multiplex polymerase chain reaction technique (m-PCR). From October 2021 to May 2022, one-handed eighty-five Blood samples were drawn randomly from sheep in various regions of Mosul city. The overall prevalence of OT was 42% (22.7 out of 185) and 52.4% (97 out of 185) using microscopic examination and c-PCR technique, respectively. A slight agreement was observed between ME of blood smears and c-PCR technique according to Kappa value 0.190, with low sensitivity, specificity, and accuracy of ME method was 30%, 88.6%, 58.4%, respectively, compared with c-PCR technique. The prevalence of mixed infection 22.7% and single infection with T. lestoquardi 20% were significantly higher (P<0.05) than single infection with T. ovis 9.7%. This study concludes that OT is widespread in Mosul city, Iraq, and the c-PCR technique is more reliable and suitable for detecting Theileria infection in sheep than the ME method.

Clinical, seroepidemiological and hematological study of Buffaloes Babesiosis in Basrah, Iraq

“…The significant differences in the lever and kidney function test (P<0.05) came from the dysfunction and physiopathy changes in the hepatic and nephrotic tissues which normally response to these changes by increase in these enzymes values with variation that agree with (Olaa et al 2017& Mohammed et al 2020 The season and months the study detects the high infection rate in the summer because the disease is a tick born that mean the high incidence of the diseases increase with diffuse of the tick which become active in the summer such results reported by (Alsaad et al 2016) while the low incidence of the disease in the mild or cold weather months due to decrease of the tick activities in this season.…”

“…Giemsa Staining: Blood smears were prepared, and after air drying, the smears were fixed in methanol alcohols for three minutes, then stained with Giemsa stain for twenty minutes then examined with light microscope under an oil immersion lens to diagnose Babesia parasites based on parasite morphologically. (Mohammed et al 2020&Sawitri1 et al 2021 The hematological analysis included: red and white blood cells count (RBCs and WBCs count), packed cell volume (PCV), hemoglobin concentration (Hb %) and deferential leukocytes count. The serum was used in biochemical analyses that included: aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALKP), blood urea nitrogen (BUN), creatinine and total protein (TP).…”