Detection of Candida albicans Using a Manufactured Electrochemical Sensor

Abstract

Candida albicans is a commensal fungus that is responsible for a lot of nosocomial infections in immunocompromised people. Cell culture is currently the predominant method for diagnosing candidiasis, but it is time consuming. In this study, we developed a rapid screen procedure by devising a method for detecting C. albicans with the use of electrochemical sensors. Through this experiment, we propose a method for the detection of C. albicans in the system through the use of personal glucose meters. The hemicellulase was used to break down the cell wall of C. albicans to glucose and oligo, which can be detected by a glucose meter. The spiked samples were prepared suspending C. albicans in urine and serum, demonstrating the feasibility of the developed method in a real situation.

Mucormycosis diagnosis revisited: Current and emerging diagnostic methodologies for the invasive fungal infection (Review)

“…Electrochemical bio-sensors have been used to detect fungi such as Candida albicans and A. fumigatus. The relevant electrochemical biosensors for these fungi use membrane-bound impedance spectroscopy and chitosan-stabilised gold nano-particles (32). Optical biosensors to detect Candida species were developed in the study by Cai et al (33), which used Mannan on the cell surface to bind to the hydrogel Con-A.…”

A Short Review on Miniaturized Biosensors for the Detection of Nucleic Acid Biomarkers

“…The creation of linear probes uses a variety of commercially accessible firmware that may create detention of oligonucleotides inside highly conserved or hypervariable areas of various genomes following their assembly and alignment. Final tests include employing a basic local alignment search tool (BLAST) to check candidate sequences for homologies, theoretical melting temperature, dimer formation, and length (18–24 nucleotides) [ 76 , 77 , 78 , 79 ].…”

Rapid detection of Candida albicans in urine by an Electrochemical Impedance Spectroscopy (EIS)-based biosensor

“…20,21 In particular, electrochemical biosensors offer an alternative approach for detecting Candida cells by leveraging their electrochemical properties. 22 For instance, amperometric 23 and potentiometric 20 biosensors utilize the measurement of current or potential changes, respectively, upon Candida interaction. These biosensors demonstrate good sensitivity, simplicity, and cost-effectiveness, but they may suffer from limited selectivity due to non-specific interactions.…”