Detection of Campylobacter jejuni, Campylobacter coli, and virulence genes in poultry products marketed in Northeastern Brazil

Melo, Felipe Pereira de; Silva, Priscila Oliveira da; Clemente, Saruanna Millena dos Santos; Melo, Renata Pimentel Bandeira de; Silva, José Givanildo da; Júnior, José Wilton Pinheiro; Fonseca, Belchiolina Beatriz; Mendonça, Marcelo; Barros, Mércia Rodrigues

doi:10.33448/rsd-v10i10.19224

Cited by 5 publications

(2 citation statements)

References 16 publications

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“…Lower isolation rate from frozen carcasses were obtained by Borges et al (2020) as 11.1%. Contrarily higher percentages as 36.6 and 30% were obtained by Maziero andde Oliveira (2010) andDe Melo et al (2021), respectively. Differences in chicken campylobacteriosis incidence rates can be attributed to a variety of factors, including the type of samples examined, location, climate factors, hygienic measures and isolation methods, as well as identification techniques.…”

Section: Incidence Of Campylobacter Species In Humanmentioning

confidence: 86%

Prevalence and Virulence Genes Profile of Zoonotic Campylobacter species in Chickens and Human in Aswan Governorate

abbas

Karmi²,

mubarak³

et al. 2022

SVU-International Journal of Veterinary Sciences

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Campylobacteriosis is considered as one of the most common zoonotic gastrointestinal diseases in the world. Because of the substantial contamination of poultry carcasses and organs with stomach contents during mechanical evisceration, birds and their products are the most common sources of Campylobacter. This study evaluated the mutual function of chickens in the transmission of Campylobacter jejuni and Campylobacter coli to hospitalized patients in Aswan Governorate, Egypt. Samples were collected randomly from fresh chickens (no= 108) and frozen chickens (no= 100) from different supermarkets in Aswan Province, Egypt as well as 60 diarrheal samples were assembled from hospitalized patients. Biochemical and molecular techniques were employed through duplex polymerase chain reaction objecting the 23S rRNA, mapA and ceuE genes specific to genus Campylobacter, C. jejuni and C. Coli, respectively, after that virulence genes (flaA and cadF genes) were detected. By using conventional and duplex PCR methods, the overall incidence of Campylobacter was 29% and 25.4 %, respectively. C. jejuni and C. coli by conventional and PCR approaches were identified as 18.1, 5.1%, and 12.3, 7.2%, respectively, while 5.8% mixed infection was discovered by both techniques. Campylobacter species could be isolated from 66.7, 25, 17.5 and 18.3% of fresh chickens, frozen chickens, frozen liver and gizzard, and human, respectively with statistically significant difference. Epidemiologically, the insignificant age risk factor was statistically reported in this study among patients although Campylobacter was dominant in the 21-35 and 36-50 age groups. Campylobacter incidence was higher among females (33.3%) than in males (11.9%). On the other hand, flaA virulence gene was detected in 10.3% of both C. jejuni and C. coli isolated from chickens but could not be detected in human isolates. Whereas cadF virulence gene could be isolated in 20.5, 23.1, 36.4, and 9.1% of C. jejuni and C. coli of chickens′ and human isolates, respectively. In conclusion, the high incidence of Campylobacter in fresh chickens is considered the main risk factor for domestically acquired campylobacteriosis in Aswan Governorate, Egypt, confirming the urgent need for food safety strategies and emphasizing the importance of refrigeration and freezing in controlling bacterial growth in foods.

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Section: Incidence Of Campylobacter Species In Humanmentioning

confidence: 86%

Prevalence and Virulence Genes Profile of Zoonotic Campylobacter species in Chickens and Human in Aswan Governorate

abbas

Karmi²,

mubarak³

et al. 2022

SVU-International Journal of Veterinary Sciences

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“…The consumption of tainted poultry products is frequently associated with outbreaks. Poultry is considered a reservoir host of C. jejuni because this pathogen is found mostly in the gastrointestinal system of chickens and has been linked to meat contamination during processing (Beiranvand et al, 2022 ; de Melo et al, 2021 ). Poultry products are a popular source of proteins, vital amino acids, mineral salts, and vitamins for many individuals (Alagawany et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Campylobacterspp. isolated from poultry in Iran: Antibiotic resistance profiles, virulence genes, and molecular mechanisms

Mousavinafchi

Rahimi

Shakerian

2022

Food Science & Nutrition

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Campylobacter spp. genera is one of the most common causes of microbial enteritis worldwide. The objective of this work was to investigate the antimicrobial resistance (AMR) patterns, virulence genes, and genetic variation of thermophilic Campylobacter species collected from chicken meat samples in Iran. A total of 255 meat specimens were taken and transferred to the laboratory. Culture methods were utilized to identify the Campylobacter genus, and PCR and sequencing were performed to confirm the organisms. Antimicrobial susceptibility evaluation was performed using broth microdilution for six antimicrobials [ciprofloxacin (CIP), nalidixic acid (NAL), sitafloxacin (SIT), erythromycin (ERY), tetracycline (TET), and gentamicin (GEN)]. By using PCR, AMR and virulence genes were detected. The detection rate of Campylobacter spp. was 64 (25.09%) out of 255 meat samples, with C. jejuni and C. coli accounting for 41 (64.06%) and 14 (21.87%), respectively. Other Campylobacter isolates accounted for 14.06% of the total (nine samples). The antibiotic susceptibility of all Campylobacter isolates was tested using six antibiotics, and all (100%) were resistant to CIP and NAL. However, TET resistance was observed in 93.9% and 83.3% of C. jejuni and C. coli isolates, respectively. Four (8.2%) C. jejuni isolates were multidrug‐resistant (MDR), while none of the C. coli isolates were MDR. Two of the four MDR isolates were resistant to CIP, NAL, TET, and ERY, whereas the other two isolates were resistant to CIP, NAL, TET, and GEN. The values of the Minimum Inhibitory Concentration (MIC) were as follows: CIP, 64–256 μg/ml; NAL, 128–512 μg/ml; TET, 2–1024 μg/ml; SIT, 0.25–1 μg/ml; ERY, 1–32 μg/ml; and GEN, 1–256 μg/ml. recR , dnaJ , cdtC , cdtB , cdtA , flaA , ciaB , cadF , and pidA were discovered in more than 50% of C. jejuni isolates, although wlaN , virbll , cgtB , and ceuE were found in <50%. flaA , cadF , pidA , and ciaB were discovered in more than 50% of the C. coli samples, whereas recR , cdtC , cdtB , cdtA , and cgtB were found in less than half. For C. coli , the percentages for wlaN , dnaJ , virbll , an...

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Overview of Virulence and Antibiotic Resistance in Campylobacter spp. Livestock Isolates

Bunduruș¹,

Balta²,

Ştef³

et al. 2023

Antibiotics

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Campylobacter remains the most prevalent foodborne pathogen bacterium responsible for causing gastroenteritis worldwide. Specifically, this pathogen colonises a ubiquitous range of environments, from poultry, companion pets and livestock animals to humans. The bacterium is uniquely adaptable to various niches, leading to complicated gastroenteritis and, in some cases, difficult to treat due to elevated resistance to certain antibiotics. This increased resistance is currently detected via genomic, clinical or epidemiological studies, with the results highlighting worrying multi-drug resistant (MDR) profiles in many food and clinical isolates. The Campylobacter genome encodes a rich inventory of virulence factors offering the bacterium the ability to influence host immune defences, survive antimicrobials, form biofilms and ultimately boost its infection-inducing potential. The virulence traits responsible for inducing clinical signs are not sufficiently defined because several populations have ample virulence genes with physiological functions that reflect their pathogenicity differences as well as a complement of antimicrobial resistance (AMR) systems. Therefore, exhaustive knowledge of the virulence factors associated with Campylobacter is crucial for collecting molecular insights into the infectivity processes, which could pave the way for new therapeutical targets to combat and control the infection and mitigate the spread of MDR bacteria. This review provides an overview of the spread and prevalence of genetic determinants associated with virulence and antibiotic resistance from studies performed on livestock animals. In addition, we have investigated the relevant coincidental associations between the prevalence of the genes responsible for pathogenic virulence, horizontal gene transfer (HGT) and transmissibility of highly pathogenic Campylobacter strains.

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Detection of Campylobacter jejuni, Campylobacter coli, and virulence genes in poultry products marketed in Northeastern Brazil

Cited by 5 publications

References 16 publications

Prevalence and Virulence Genes Profile of Zoonotic Campylobacter species in Chickens and Human in Aswan Governorate

Prevalence and Virulence Genes Profile of Zoonotic Campylobacter species in Chickens and Human in Aswan Governorate

Campylobacterspp. isolated from poultry in Iran: Antibiotic resistance profiles, virulence genes, and molecular mechanisms

Overview of Virulence and Antibiotic Resistance in Campylobacter spp. Livestock Isolates

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