Detection of C-Reactive Protein in Serum Using Resonant Property of Quartz Crystal Microbalance

Aizawa, Hitoshi; Kurosawa, Shigeru; Tozuka, Mitsuhiro; Kurosawa, Chikako; Park, Jong-Won; Noda, Kohei; Tao, Hiroaki

doi:10.5796/electrochemistry.74.156

Cited by 2 publications

(2 citation statements)

References 17 publications

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Section: Introductionmentioning

confidence: 99%

“…At present, sensors that use a quartz crystal microbalance [1][2][3][4][5] or surface plasmon resonance phenomena [6][7][8][9][10] have been reported as methods for successful detection of biomarkers. The former has successfully detected C-reactive protein (CRP), which is a biomarker of cardiovascular disease, in 2006 [5], and the latter has detected a prostate specific antigen (PSA), known to be a cancer marker, from 2004 to 2008 [6][7][8][9][10]. However, because these sensors use bulk quartz or prisms, it is difficult to simultaneously detect multiple types of biomarkers on one sensor chip, and it becomes expensive to examine because the amount of antibody reagent required for one measurement increases.…”

Section: Introductionmentioning

confidence: 99%

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Surface stress sensor based on MEMS Fabry–Perot interferometer with high wavelength selectivity for label-free biosensing

Takahashi

Hizawa

Misawa³

et al. 2018

J. Micromech. Microeng.

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We have developed a surface stress sensor based on a microelectromechanical Fabry–Perot interferometer with high wavelength selectivity by using Au half-mirrors, for highly sensitive label-free biosensing. When the target molecule is adsorbed by the antigen–antibody reaction onto a movable membrane with a thin Au film, which acts as an upper mirror of the optical interferometer, the amount of deflection of the movable membrane deflected by the change in surface stress can be detected with high sensitivity. To improve the signal at the small membrane deflection region of this biosensor resulting in detection of low concentration molecules, by integrating 50 nm-thick Au half-mirrors, the wavelength selectivity of the optical interferometer has been successfully improved 6.6 times. Furthermore, the peak shift in the reflection spectrum due to the adsorption of bovine serum albumin (BSA) antigen with a concentration of 10 ng ml−l by the antigen–antibody reaction was spectroscopically measured on the fabricated optical interferometer, and the deflection amount of the movable membrane after 10 min treatment was 2.4 times larger than that of nonspecific adsorption with the avidin molecules. This result indicated that the proposed sensor can be used for selective detection of low-concentration target antigen molecules.

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