Detection of Brucella spp. in Dairy Products by Real-Time PCR

Moslemi, Elham; Soltandalal, Mohammad Mehdi; Beheshtizadeh, Mohammad Reza; Taghavi, Afsoon; Manjili, Hamidreza Kheiri; Lamouki, Reza Mahmoudi; Izadi, Azimeh

doi:10.5812/archcid.12673

Cited by 9 publications

(4 citation statements)

References 29 publications

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“…These findings are harmonious with published similar studies from Pakistan (14), Iraq (10,13,15), and India (16). On the other hand, higher rates of anti-Brucella antibodies were documented in studies in Libya 21% (17), India 27% (18), Egypt 36.4%-56.2% (19), and Iran 45% (20). Such variations might be attributed to various factors including the extent of encounters with wildlife (natural reservoirs), seasonality, level of available veterinary care, and testing methodology (10,13).…”

Section: Discussionsupporting

confidence: 83%

“…A Nigerian study also detected a similar prevalence in livestock by the bacteriological approach (21). Besides, different rates of Brucella contaminating sheep and goats' raw milk were found in Iran ranged from 1.2% to 45.5% (20,22). In addition to seasonal and epidemiological variations in different regions, isolation of Brucella is also influenced by the bacteriological protocol.…”

Section: Discussionmentioning

confidence: 83%

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Diagnosis of brucellosis in sheep and goats raw milk by fast and reliable techniques

Almashhadany¹

2021

IJVS

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This study aimed to evaluate the occurrence of brucellosis in sheep and goats' raw milk samples by detecting anti-Brucella antibodies and Brucella species at Erbil governorate. A total of 320 raw milk samples (150 sheep milk and 170 goats milk) were irregularly collected from dairy females. The overall occurrence of Brucella antibodies in samples of sheep and goats raw milk was 11.6% and 9.7% according to MRT and indirect ELISA, respectively. Isolation of Brucella (B.) species from samples of raw milk was 7.8%. The isolated species of Brucella were B. melitensis and B. abortus. A noticeable increase in frequency during September to November was observed. In conclusion, brucellosis is still a considerable public health threat in the Erbil. Based on the tests performance, the study recommends MRT in standard observing of brucellosis in milk aggregate farms, centers, and dairy manufactories. Customers are also recommended to adequately pasteurize the milk in order to damage this milk-borne pathogen before ingestion or saleable handling.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 83%

Diagnosis of brucellosis in sheep and goats raw milk by fast and reliable techniques

Almashhadany¹

2021

IJVS

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“…The analytical detection limit of the qPCR based on this target ranged from 1 to 6 genome copy/g in cheese [ 17 ]. Previous studies in India and Iran using PCR and quantitative real-time PCR showed high contamination rates in raw goat milk (88.8% and 45.5%, respectively) [ 18 , 19 ]. While using the milk ring test or ELISA, the contamination rates in cow′s milk ranged between 4.4 to 5.8% in India [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

Brucella spp. Contamination in Artisanal Unpasteurized Dairy Products: An Emerging Foodborne Threat in Tunisia

Béjaoui

Abdallah

Maaroufi

2022

Foods

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Brucellosis is a worldwide zoonotic disease transmitted to humans, predominantly by the consumption of contaminated raw milk and dairy products. This study aimed to investigate the occurrence of Brucella spp. in 200 raw milk, ricotta, and artisan fresh cheese samples, collected from individual marketing points in four districts in Tunisia. Samples were analyzed for the presence of Brucella spp. by IS711-based real-time PCR assay. Positive samples were further analyzed by qPCR for B. melitensis and B. abortus species differentiation. The DNA of Brucella spp. was detected in 75% of the samples, B. abortus was detected in 31.3%, and B. melitensis was detected in 5.3% of positive samples. A percentage of 49.3% of samples co-harbored both species, while 14% of the Brucella spp. positive samples were not identified either as B. abortus or B. melitensis. High contamination rates were found in ricotta (86.2%), cheese (69.6%), and raw milk (72.5%) samples. The study is the first in Tunisia to assess the occurrence of Brucella spp. contamination in artisanal unpasteurized dairy products and showed high contamination rates. The detection of both B. abortus and B. melitensis highlights that zoonotic high-pathogen agent control remains a challenge for food safety and consumer health protection and could represent a serious emerging foodborne disease in Tunisia.

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“…However, Brucella reservoirs still exist in the wild [16,92]. Te recent studies conducted in India, Iran, and Qatar using PCR techniques have confrmed the presence of Brucella species in milk and other dairy products [18,55,66]. Tis is a signifcant concern as humans can contract Brucella infection by consuming contaminated milk (Table 1).…”

Section: Brucella Prevalence In Milk and Various Dairy Productsmentioning

confidence: 99%

Presence of Brucella spp. in Milk and Dairy Products: A Comprehensive Review and Its Perspectives

Islam

Rahman

et al. 2023

Journal of Food Quality

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Consuming raw milk and milk-based products that have not been produced under strict control conditions can cause brucellosis, a highly contagious zoonotic disease. It is a significant global public health concern, particularly in regions with poor management and limited resources, such as Latin America, North and East Africa, the Middle East, and South and Central Asia. The study aims to summarize the occurrence of human brucellosis linked to milk and milk products and the presence of Brucella species in dairy foods. To achieve this goal, a meta-analysis was conducted on 69 studies ranging from 2001 to 2022, which were categorized into two groups: the incidence of Brucella species in milk and milk products and the prevalence of human brucellosis resulting from the consumption of contaminated milk. The following milk and milk products showed the highest incidence of Brucella species: cow milk (1.86%–81.7%), buffalo milk (10.4%–61.67%), camel milk (0%–24%), goat milk (0%–88.8%), and cheese (0%–39.1%). Consuming unpasteurized milk and milk products has been identified as the leading cause of human brucellosis, with incidence rates varying from 33.9% to 100%. Several human brucellosis cases have been linked to consuming raw milk and cheese in Spain, Israel, and other countries. Various serological techniques are employed to detect Brucella-specific antibodies in milk. The milk ring test (MRT) and enzyme-linked immunoassay (ELISA) are the two most widely utilized methods for detecting these antibodies in milk. Recently developed dual biosensors are a powerful approach for early diagnosis of Brucella from milk. Real-time PCR can rapidly detect organisms, reducing the risk of lab contamination and false positive results. To prevent and control brucellosis, essential steps include proper pasteurization of milk and dairy products, using the milk ring test (MRT) to detect Brucella in individual and bulk milk, immunization, education, and increasing public awareness of the disease. The consumption of raw milk and milk-made products that are not produced under strictly controlled conditions poses a significant risk to human health, mainly due to the high incidence of Brucella contamination. Therefore, ensuring strict control measures in producing milk and milk-made products is crucial to preventing the spread of this disease and safeguarding human health.

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Detection of Brucella spp. in Dairy Products by Real-Time PCR

Cited by 9 publications

References 29 publications

Diagnosis of brucellosis in sheep and goats raw milk by fast and reliable techniques

Diagnosis of brucellosis in sheep and goats raw milk by fast and reliable techniques

Brucella spp. Contamination in Artisanal Unpasteurized Dairy Products: An Emerging Foodborne Threat in Tunisia

Presence of Brucella spp. in Milk and Dairy Products: A Comprehensive Review and Its Perspectives

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