Detection of Brucella Antibodies in Sheep with Special Aspect of Clinicalstatus and Breed

García-Martínez

et al. 2023

J Hellenic Vet Med Soc

The seroprevalence of Brucella antibodies in serum of goats and sheep reared on semi-arid pastoral systems of northeastern Mexico was assessed. Additionally, species, gender, and predominant breed were evaluated as risk factors for seropositivity to brucellosis. Data were from 375 flocks of goats or co-mingled goats and sheep. Serum samples from 11001 goats and 4741 sheep collected between 2016 and 2019 were analyzed with the brucellosis card test. The disease affected 12% of flocks. The overall prevalence of brucellosis seropositivity in goats and sheep were estimated at 2.1% (95% CI = 1.78-2.31) and 0.8% (95% CI = 0.58-1.10), respectively. Brucellosis seropositivity was two times more likely (p < 0.01) in goats than sheep. The risk of seropositivity to brucellosis for goats and sheep was 2.3 and 3.2 times higher (p < 0.01) in females than males. Toggenburg goats were more likely (p < 0.01) to be seropositive to brucellosis than all other breeds of goats, whereas criollo sheep were more likely to be seropositive to brucellosis compared to hair sheep composite breeds. The current study revealed that, despite the absence of Brucella vaccination, brucellosis is not widely distributed in the study area. Also of all animals screened, seropositivity to Brucella infection was highest in Toggenburg when compared to other dairy and meat breeds; Criollo sheep also presented the highest seropositivity to brucellosis compared to hair sheep composite breeds. Finally, females compared to males had increased odds of testing positive for brucellosis.

Section: Resultsmentioning

confidence: 99%

Sero-epidemiology of brucellosis in small ruminants on rangeland in northern Mexico

García-Martínez

et al. 2023

J Hellenic Vet Med Soc

“…Different species of Brucella tend to be hostspecific.Brucellae aregram-negative coccobacilli with straight or slightly curved, facultative intracellular,nonspore forming, non-motile, and non-capsulated cells (Waringa et al, 2016;Patel et al, 2017). Out of twelve species, the most implicated agents of brucellosis are B.abortus, B. melitensis, B. bovis, B.canis, and B.suis (Scholz et al, 2016;Sabrina et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Application of Rose Bengal Test and ELISA in Meat Juice for Monitoring of Brucellosis among Cattle Carcasses at Erbil City, Iraq

mashhadany¹

2019

BUASVMCN-ASB

Brucellosis is a global zoonotic bacterial disease. It is also an ever-increasing public health concern, particularly in endemic regions such as Kurdistan region in Middle East. Samples of meat juice were screened for anti-Brucella antibodies via Rose Bengal Test (RBT) and ELISA from January to June 2018. Bacteriological isolation of Brucella sp. from meat samples was also performed. The overall prevalence of bovine brucellosis is 7.7%, 6.6%, and 4.9% by RBT, ELISA, and culture-based test respectively. Based on sensitivity and specificity, ELISA outperforms RBT in comparison to culture results as the gold standard test. Nonetheless, both tests showed good efficiency in comparison to culture approach. In terms of temporal changes of brucellosis rate, spring progress is strongly associated (r²=0.96) with increase in seroprevalence. In conclusion, the prevalence of bovine brucellosis in Erbil city is alarming. Countermeasures should be taken to mitigate the economic losses and transmission to human.

“…Brucellae are gram-negative coccobacilli or short rods, facultative intracellular, non -motile, nonspore forming, urease + ive, Brucella members are aerobic, but some strains require an atmosphere containing 5 to 10% Co2 added for growth, especially on primary isolation and non-capsulated [13,14].Currently, there are eleven species described in the genus Brucella, every one may infect different host species, but each Brucella species has a favorite for its host species. Six classical out of eleven species include Brucella abortus, Brucella melitensis, Brucella ovis, Brucella canis, Brucella suis, Brucella neotomae and five novel species of BrucellaincludeBrucellaceti,Brucellapinnipedialis,Brucellamicroti,Brucellainopinata,Brucel la papionis [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Untitled

2018

RJLBPCS

ABSTRACT:This study was conducted to monitoring human brucellosis among Erbil Governorate.A total of 280 blood samples were collected from male (146) and female (134). Information about persons was recorded, including sex, age, and place of habitation. These samples comprised 155 and 125 blood samples from rural and urban area respectively. The overall prevalence of brucella antibodies was (8.6%).The highest rate was found in female (9.7%), while the lowest rate was from the male (7.5 %). The prevalence rate among male was high (10.3 %) in age group 31-40 years, while in females were high (19.2%) in age group 21-30 years. Out of 280 blood samples, only (5.7%) brucella isolates were found. The isolation of Brucella abortus were (57.1%) and (66.7 %), while (42.9%) and (33.3%) were Brucella melitensis from male and female respectively. The relation between result of RBT and isolation of Brucella species achieved that (7.5 %) and (9.7%) from male and female were positive according to RBT, compared with (4.8%) and (6.7%) gave isolates of Brucella species respectively. According to the habitation of the participants, high prevalence rate (9.7%) among Rural area, whereas (7.2%) among urban area. The highest rate were found in September (12.0%), while the lowest rate were in July (6.7%).This study clarified that brucellosis is still one of a significant public health challenge for Iraqi Kurdistan. We recommend that the RBT play significant role in monitoring of human brucellosis.