Detection of bovine tuberculosis in three dairy cow stations in Iraq

Al-Graibawi, Mawlood Abbas Ali; Mohammad, T.A.; Barak, S.S.S.

doi:10.33899/ijvs.2014.116888

Cited by 2 publications

(4 citation statements)

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“…The isolation rate of M. bovis for non-visible reactors was 1 (9.09%). The total recovery rate of M. bovis for both visible and nonvisible reactors was 50 (64.93%), these results agreed with (22,24,53,54). and the number of atypical mycobacteria isolates was 3 (3.9%) which agree with (26,54).…”

Section: Discussionsupporting

confidence: 76%

Prevalence and risk factors of bovine tuberculosis in dairy cattle farms in Egypt

Hamed¹,

Nasr²,

Azooz³

et al. 2021

IJVS

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A cross-sectional study was carried out from November, 2015 to April, 2018 to determine the prevalence and identify the risk factors of BTB using the comparative intradermal tuberculin (CIDT) test on 5327 dairy cattle in 16 dairy herds in mid-delta, Alexandria Road and Upper Egypt districts. Questionnaires used to collect data on herd size and management. The herd and individual animal prevalence at cut-off ≥4mm were 68.75% (95%, CI: 46 to 91.4) and1.67% (95%, CI: 1.3to 2.1) respectively. The individual prevalence was significantly associated with age, breed and different location of farms and density of cattle in yard. On herd level, history of BTB in the farm, management practices, raising of different species in the same facility and newly purchased cattle are important risk factors for BTB. The postmortem examination of positive reactors revealed 66 (85.71%) out of 77slaughtered cattle with visible lesions and 11(14.29%) with non-visible lesions. Bacteriological examination revealed 74.24% (49/66) from visible lesions and 9.09% (1/11) from non-visible lesions were Mycobacterium bovis. The indirect enzyme linked immunosorbent assay (IELISA) results revealed 31(40.26%) showed positive result including 29 (93.6%) visible lesions and 2(6.4%) nonvisible lesions. In conclusion, the prevalence high in herds with poor management condition, raised different species in the same facility, had previous history of BTB infection and purchased new animals. At animal level Holstein breed, 3-6 years age, kept in high density and located in mid-delta district were at higher risk to be reactor to comparative intradermal tuberculin (CIDT) test.

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Section: Discussionsupporting

confidence: 76%

Prevalence and risk factors of bovine tuberculosis in dairy cattle farms in Egypt

Hamed¹,

Nasr²,

Azooz³

et al. 2021

IJVS

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“…Four weeks post the immunization; the left flank area of the lamb was clipped and shaved carefully, and divided into two portions, the firstone was injected I.D with 0.1 mL of normal saline as a control, while the second one were injected with 0.1 mL of purified protein derivative (PPD). The thickness of skin reaction was assayed by the ruler at 72 hours after inoculation (15), TF was extracted from spleen cell of the lambs.…”

Section: Delayed Type Hypersensitivity-skin Testmentioning

confidence: 99%

“…To estimate the safety and biological activity of the prepared TF to transfer DTH-reaction, six rabbits were divided equally in to 2 groups, the first groups injected intra-peritoneal (I.P) with 2 mL equivalent to 5*10 8 cells/mL of specific TF and the second group injected I.P with 2 mL normal saline and monitored daily for any clinical signs and checked for cellular immunity by DTH test one week later (15).…”

Section: First Experimentsmentioning

confidence: 99%

“…Clinical examination was carried out to all animals at zero time and post injection, which includes heart rate, rectal temperature and respiratory rate, blood sample were collected for 3 times post 2 nd immunization for serum gathering. All the lambs were examined by DTH-skin test as described above at the 4 th week post immunization (15).…”

Section: Second Experimentsmentioning

confidence: 99%

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Clinical and immunological study of specific Mycobacterium bovis-BCG transfer factor in lambs

Nayyef¹,

Al-Graibawi²

2021

IJVS

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This study was designed to prepare and evaluate the specific Mycobacterium bovis (M. bovis) Bacillus Calmette-Guerin (BCG) transfer factor (TF) from spleen cells of two lambs vaccinated subcutaneously S/C with BCG vaccine twice 2 weeks' intervals, similarly one lamb injected with normal saline used as a donor of non-specific TF. The lambs were clinically examined at zero time and along 3 days post each immunization, then, delayed type hypersensitivity (DTH) was checked post 4 weeks. TF was obtained from spleen cells of the lambs, inspected for sterility; safety.TF was evaluated in 4 groups (five in each) of lambs 3-4 months old. The first group (recipient) was injected S/C with 2 ml of specific BCG-TF, similarly the 2nd, 3rd and 4th groups were injected with nonspecific TF, BCG and normal saline respectively. The clinical signs and DTH test were checked as described above, only the specific TF recipient and BCG vaccinated lambs gave a positive DTH test. The serum concentration of inerlukin-17 (IL-17), interferongamma (IFN-γ) and migration inhibitory factor (MIF) cytokines were measured by Enzyme-linked immunosorbent assay (ELISA), which revealed a highly significant increase in the TF recipient lambs in comparison with the control and vaccinated groups. It concluded that the prepared specific M. bovis-BCG transfer factor was effective to induce cell mediated immunity via DTH and increasing the serum concentration of cytokines in recipient lambs.

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Detection of bovine tuberculosis in three dairy cow stations in Iraq

Cited by 2 publications

References 0 publications

Prevalence and risk factors of bovine tuberculosis in dairy cattle farms in Egypt

Prevalence and risk factors of bovine tuberculosis in dairy cattle farms in Egypt

Clinical and immunological study of specific Mycobacterium bovis-BCG transfer factor in lambs

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