Detection of bovine papillomavirus DNA on the normal skin and in the habitual surroundings of horses with and without equine sarcoids

Bogaert, Lies; Martens, Ann; Baere, Cindy De; Gasthuys, Frank

doi:10.1016/j.rvsc.2004.12.003

Cited by 65 publications

(49 citation statements)

References 22 publications

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“…Because there were approximately 40 papillomatosis-affected cattle on the farm where the two horses were kept, we believe that these sarcoid-affected horses were likely infected by direct or indirect contact with infected cattle (29). The previous identification of the BPV/BR-UEL4 strain in cattle warts collected from two other farms in nearby cities corroborates this speculation (21).…”

Section: Discussionmentioning

confidence: 69%

“…The identification of BPV13 in the sarcoids examined in this study gives rise to the hypothesis that a fraction of PV isolates previously diagnosed from sarcoids worldwide by PCRs using BPV1/2 E5-specific primers might have been misclassified as BPV2, especially as direct sequencing of numerous amplicons was not performed (10,15,(29)(30)(31). To avoid the misidentification of PV types, the use of general PCRs with broad-spectrum primers and sequencing is recommended.…”

Section: Discussionmentioning

confidence: 99%

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Bovine Papillomavirus Type 13 DNA in Equine Sarcoids

et al. 2013

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bEquine sarcoids are locally aggressive fibroblastic neoplasms considered to be the most common skin tumors of horses worldwide. Bovine papillomavirus types 1 and 2 have typically been associated with sarcoids in equids. Investigations aiming to identify papillomavirus strains, aside from bovine papillomaviruses 1 and 2, which might be associated with sarcoid lesions, have been lacking. The aim of this article is to report the identification of a third bovine papillomavirus type, bovine papillomavirus 13, associated with equine sarcoids. Six sarcoid lesions were collected from diverse anatomical sites on two horses from southern Brazil. To detect a broad spectrum of papillomavirus strains, eight degenerate primer pairs designed to detect conserved regions on the L1 and E1 genes were tested on the DNA samples. Direct sequencing was then performed on the obtained amplicons, and sequence identities were compared with sequences from all bovine papillomavirus types. The FAP59/FAP64, MY09/MY11, and AR-E1F2/AR-E1R4 sequences generated from the sarcoids were shown to present 99 to 100% identity with bovine papillomavirus 13, a new bovine papillomavirus type previously described in cattle. The results from this study suggest that there is a need to identify bovine papillomavirus type 13 and other papillomavirus strains that might be associated with sarcoids in diverse geographical areas; such investigations might establish the frequency of occurrence of this viral type in these common tumors of equids.

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Section: Discussionmentioning

confidence: 69%

Section: Discussionmentioning

confidence: 99%

Bovine Papillomavirus Type 13 DNA in Equine Sarcoids

et al. 2013

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“…Bovine papillomaviruses (BPVs) belong to Papillomaviridae (1), a family of viruses that have oncogenic potential to cause benign and malignant tumors in association with proliferations of cutaneous or mucosal epithelia (2,3). Recently, bovine papillomaviruses have been classified into three genera: BPVs 1, 2, and 13 as Deltapapillomavirus; BPVs 5 and 8 as Epsilonpapillomavirus; and BPVs 3, 4, 6, 9, 10, 11, and 12 as Xipapillomavirus.…”

Section: Introductionmentioning

confidence: 99%

“…BPV- 7 has not yet been assigned to a genus (1,4). BPV-1 and BPV-2 have also been identified from equine sarcoids in horses as a result of interspecies transmission (2,3). The presence of BPV-2 DNA has been identified in alimentary fibropapillomas, cutaneous papillomas, and urinary bladder cancer (5,6).…”

Section: Introductionmentioning

confidence: 99%

“…The presence of BPV-2 DNA has been identified in alimentary fibropapillomas, cutaneous papillomas, and urinary bladder cancer (5,6). BPV types 1 and 2 have also been identified in peripheral blood leukocytes (7,8), and in different body fluids, such as milk, colostrum, and semen, obtained from animals with papillomatosis (5,9,10), and even from apparently healthy cattle (8,11) and horses (2,12). BPV-4 is associated with cutaneous papillomatosis, besides upper-gastrointestinal papillomatosis (6,8,10).…”

Section: Introductionmentioning

confidence: 99%

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