Detection of Bordetella pertussis in Infants Suspected to have Whooping Cough

Hajia, Massoud; Rahbar, Mohammad; Fallah, Fatemeh; Safadel, Nooshafarin

doi:10.2174/1874306401206010034

Cited by 15 publications

(8 citation statements)

References 19 publications

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“…In this study, B. pertussis was detected only in 18% of suspected cases. In the study conducted by Hajia et al (2012) from Tehran, Iran, 12 out of 138 children < 6 months (9%) were positive for B. pertussis, which was lower compared to the current result. Castillo et al (2015) from Peru reported an incidence of pertussis as high as 40% in patients younger than one year admitted to five hospitals in Lima, Peru, which was higher than the rate of this study.…”

Section: Discussioncontrasting

confidence: 85%

Identification of Etiologic Agents of the Pertussis-like Syndrome in Children by Real-time PCR Method

et al. 2018

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The aim of this study was to recognize the identity and frequency of etiologic agents of the pertussis-like syndrome in children < 2 years of age. A cross-sectional hospital-based study conducted from August 2014 to August 2015. All children < 2 years of age (n=100) who were suspected as pertussis infected were enrolled in this study and tested for Bordetella pertussis, adenovirus (Adv), respiratory syncytial virus (RSV), human metapneumovirus (hMPV), and influenza virus A (INF-A) by real-time PCR technique. RSV was the most detected pathogen (20%), followed by B. pertussis (18%), Adv (16%), INF-A (11%), and hMPV (10%). Co-infection was observed in 8 patients (11%) and the combinations of RSV/INF-A (n=3, 4%), and AdV/B. pertussis (n=3, 4%) were more frequent. RSV, B. pertussis, and hMPV were more frequent pathogens among infants < 4 months of age. However, Adv and INF-A were more frequent pathogens among children > 6 months of age. In this study, RSV was the most frequent identified pathogen (n=20, 20%), followed by B. pertussis (n=18, 18%) and AdV (n=16, 16%). Pertussis was more frequent in spring (8%) and summer (6%). In addition, clinical symptoms of pertussis were the same as some viral pathogens, which can lead to misdiagnosis of infection. Therefore, diagnosis of pertussis should be established on the bases of both the clinical symptoms and the laboratory methods.

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Section: Discussioncontrasting

confidence: 85%

Identification of Etiologic Agents of the Pertussis-like Syndrome in Children by Real-time PCR Method

et al. 2018

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“…The classic presentation of pertussis is well known, but it has been observed less often since the start of immunization [3]. Therefore, B. pertussis infection cannot be accurately diagnosed just by clinical presentations; laboratory tests are needed for confirmation of the diagnosis in those patients showing suggestive clinical signs and symptoms, or those with a history of exposure to infected patients [3,8,9].…”

Section: Introductionmentioning

confidence: 99%

“…PCR assays are faster, highly sensitive, and have shown to increase by approximately 19% the overall percentage of laboratory-confirmed cases of pertussis [9,12,13]. PCR is included in the Centers for Disease Control and Prevention (CDC) and World Health Organization (WHO) laboratory definition of confirmed cases, and its use in epidemiological surveillance has increased recently due to better capacity to measure the impact of the disease in the community, and to improved studies of vaccine efficacy [9][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Bordetella pertussis diagnosis in children under five years of age in the Regional Hospital of Cajamarca, Northern Peru

Valle-Mendoza

Oré

Petrozzi-Helasvuo³

et al. 2015

J Infect Dev Ctries

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Introduction: Bordetella pertussis is an important human pathogen that causes whooping cough (pertussis), an endemic illness responsible of significant morbidity and mortality, especially in infants and children. Worldwide, there are an estimated of 16 million cases of pertussis, resulting in about 195,000 child deaths per year. In Peru, pertussis is a major health problem that has been on the increase despite immunization efforts. The objective of this study was to determine the prevalence of B. pertussis among children under five years of age suspected to have whopping cough in Cajamarca, Peru. Methodology: Children diagnosed with whooping cough admitted to the Hospital Regional de Cajamarca from August 2010 to July 2013 were included. Nasopharyngeal samples were obtained for B. pertussis culture and polymerase chain reaction (PCR) detection. Results: In 133 children, the pertussis toxin and IS481 gene were detected in 38.35% (51/133) of the cases by PCR, while only 9.02% (12/133) of the Bordetella cultures were positive. The most frequent symptoms in patients with positive B. pertussis were paroxysm of coughing 68.63% (35/51), cyanosis 56.86% (29/51), respiratory distress 43.14% (22/51), and fever 39.22% (20/51). Pneumonia and acute bronchial obstructive syndrome were present in 17.65% (9/51) and 13.72% (7/51) of the cases, respectively. Conclusions: B. pertussis is responsible for an important proportion of whooping cough in hospitalized children in Cajamarca. Epidemiologic surveillance programs for B. pertussis are essential in Peru, especially in children who could most benefit from the vaccine.

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“…Thus, we conducted a previous study on Peruvian children with a probable diagnosis of Pertussis and reported a Bordetella pertussis prevalence of 39.5% [14]. However, the classical presentation of pertussis has proven to be not enough to achieve a definitive diagnosis and laboratory tests are of the utmost importance for etiological confirmation to avoid overdiagnosis [28,31,32]. In the light of possible coinfections and more than 60% of patients without an etiological identification in our previous study, we conducted a new comprehensive analysis to detect viral and atypical bacterial etiologies in all our patients.…”

Section: Discussionmentioning

confidence: 99%

Identfication of viral and bacterial etiologic agents of the pertussis-like syndrome in children under 5 years old hospitalized

et al. 2019

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BackgroundAcute respiratory infections (ARIs) represent an important cause of morbidity and mortality in children, remaining a major public health concern, especially affecting children under 5 years old from low-income countries. Unfortunately, information regarding their epidemiology is still limited in Peru.MethodsA secondary data analysis was performed from a previous cross-sectional study conducted in children with a probable diagnosis of Pertussis from January 2010 to July 2012. All samples were analyzed via Polymerase Chain Reaction (PCR) for the following etiologies: Influenza-A, Influenza-B, RSV-A, RSV-B, Adenovirus, Parainfluenza 1 virus, Parainfluenza 2 virus, Parainfluenza 3 virus, Mycoplasma pneumoniae and Chlamydia pneumoniae.ResultsA total of 288 patients were included. The most common pathogen isolated was Adenovirus (49%), followed by Bordetella pertussis (41%) from our previous investigation, the most prevelant microorganisms were Mycoplasma pneumonia (26%) and Influenza-B (19.8%). Coinfections were reported in 58% of samples and the most common association was found between B. pertussis and Adenovirus (12.2%).ConclusionsThere was a high prevalence of Adenovirus, Mycoplasma pneumoniae and other etiologies in patients with a probable diagnosis of pertussis. Despite the presence of persistent cough lasting at least two weeks and other clinical characteristics highly suspicious of pertussis, secondary etiologies should be considered in children under 5 years-old in order to give a proper treatment.Electronic supplementary materialThe online version of this article (10.1186/s12879-019-3671-6) contains supplementary material, which is available to authorized users.

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Detection of Bordetella pertussis in Infants Suspected to have Whooping Cough

Cited by 15 publications

References 19 publications

Identification of Etiologic Agents of the Pertussis-like Syndrome in Children by Real-time PCR Method

Identification of Etiologic Agents of the Pertussis-like Syndrome in Children by Real-time PCR Method

Bordetella pertussis diagnosis in children under five years of age in the Regional Hospital of Cajamarca, Northern Peru

Identfication of viral and bacterial etiologic agents of the pertussis-like syndrome in children under 5 years old hospitalized

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