Identification of Etiologic Agents of the Pertussis-like Syndrome in Children by Real-time PCR Method

Mahmoudi, Shima; Banar, Maryam; Pourakbari, Babak; Alavi, Hediyeh Sadat; Eshaghi, Hamid; Aziz-Ahari, Alireza; Mamishi, Setareh

doi:10.14712/23362936.2018.6

Cited by 11 publications

(9 citation statements)

References 22 publications

(27 reference statements)

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“…Previous studies have shown that pertussis infection in children sometimes mimics viral RTIs, especially in infants (6,27,35). In our study, there were no significant differences regarding the clinical symptoms between the two groups.…”

Section: Discussioncontrasting

confidence: 60%

“…The FilmArray Respiratory Panel (FilmArray RP, v1.7) is able to detect 16 viral and 3 atypical respiratory pathogens, including B. pertussis, in a single test, which requires only 5 min of hands-on time and 65 min of instrument time (32). The multiplexed nature of this test enables the rapid differentiation of B. pertussis from other viruses that also cause pertussis-like syndrome, such as RSV, Adv and parainfluenza 3 (Para 3) (33)(34)(35). In addition, this method provides co-infection information for pertussisinfected patients, which may have underlying clinical significance.…”

Section: Introductionmentioning

confidence: 99%

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Identification of etiologic agents and clinical characteristics for patients suspected of having pertussis in a large Children’s Hospital in China

et al. 2019

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Background: In China, pertussis is a major health problem with an increasing incidence despite immunization efforts. Timely and accurate diagnosis is essential for the optimal management of pertussis, especially in severe cases. Methods: Nasopharyngeal swabs or sputum specimens were obtained from patients suspected of having pertussis on the day of hospitalization at Shanghai Children's Medical Center from December 01, 2016, to November 30, 2017. The specimens were tested with the FilmArray Respiratory Panel, a multiplex polymerase chain reaction (PCR) assay that detects 16 viruses, Bordetella pertussis (B. pertussis), Mycoplasma pneumoniae (M. pneumoniae), and Chlamydophila pneumoniae (C. pneumoniae).Results: Among the 140 children studied, 50.0% (70/140) were detected with a single pathogen, 45.0% (63/140) were detected with multiple pathogens, and 5.0% (7/140) had no detected pathogens. Forty-nine (35%, 49/140) patients tested positive for B. pertussis. Respiratory syncytial virus (RSV), parainfluenza virus (Para) and rhinovirus/enterovirus (Rhino/Entero) were the most prevalent pathogens in patients with pertussis-like syndrome. No significant differences between the groups with pertussis and pertussis-like syndrome were observed regarding the clinical symptoms. Severe cases were more frequently observed in unvaccinated, premature and pertussis/RSV co-infection patients.Conclusions: Our study highlights the importance of the timely and accurate diagnosis of pertussis based on both clinical symptoms and laboratory methods.

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Section: Discussioncontrasting

confidence: 60%

Section: Introductionmentioning

confidence: 99%

Identification of etiologic agents and clinical characteristics for patients suspected of having pertussis in a large Children’s Hospital in China

et al. 2019

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“…In a study of 232 children with median age of three years who presented with pertussis-like disease clinic, nasopharyngeal swab samples taken from patients were evaluated by PCR; In 9.9% of patients, hMPV was found to be positive alone and no co-infection was observed (9). In Iran between 2014 and 2015, a study that nasopharyngeal swab specimens were taken from 100 patients who applied to hospital with the symptoms of pertussis-like disease under two years of age and evalauted with PCR has showed that the incidence of hMPV was reported to be 10% and it did not accompany other infections (10).…”

Section: Discussionmentioning

confidence: 99%

A Human Metapneumovirus Case Who Presented with Pertussis Like Illness

Altuntaş¹,

Durmuş²,

Teke³

et al. 2019

J Pediatr Inf

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“…Bordetella causes this respiratory disease. Bordetella pertussis is a small Gram-negative pathogen (2,3).…”

Section: Introductionmentioning

confidence: 99%

Identification of Etiological Agents of Pertussis-Like Syndrome in Children Younger Than 5 Years Old Hospitalized in Southwestern Iran

Shamsizadeh,

Nikfar,

Bavarsadiankhah

et al. 2023

Jundishapur J Microbiol

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Background: Despite the global vaccination program, there are many new cases of pertussis in different societies annually. Objectives: This study aimed to investigate the prevalence of some microorganisms associated with pertussis-like syndrome and compare the clinical presentations between Bordetella pertussis and pertussis-like syndrome in children. Methods: Children younger than 5 years old suspected of pertussis-like syndrome were admitted to a hospital in Ahvaz, Iran, and examined from July 2018 to July 2019. Nasopharyngeal samples were evaluated using molecular methods. The studied microorganisms were the following: B. pertussis, B. parapertussis, Mycoplasma pneumoniae, Chlamydophila pneumoniae, adenovirus, respiratory syncytial virus, and parainfluenza virus type III. Results: Forty-five children were enrolled. B. pertussis was detected in 15 cases (33.3%), respiratory syncytial virus in 14 (31.1%), C. pneumoniae in 3 (6.7%), and parainfluenza virus type III in 3 (6.7%). The collected samples were negative in terms of M. pneumoniae, adenovirus, and B. parapertussis. In the case of paroxysmal cough, the clinical symptoms were significantly different between pertussis and pertussis-like groups. Conclusions: The results indicated that children with pertussis-like syndrome are commonly infected with B. pertussis and respiratory syncytial virus, so more attention should be paid to this issue. The study also demonstrated the importance of molecular diagnosis methods, along with diagnosis based on clinical symptoms, in children suspected of pertussis-like syndrome.

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Identification of Etiologic Agents of the Pertussis-like Syndrome in Children by Real-time PCR Method

Cited by 11 publications

References 22 publications

Identification of etiologic agents and clinical characteristics for patients suspected of having pertussis in a large Children’s Hospital in China

Identification of etiologic agents and clinical characteristics for patients suspected of having pertussis in a large Children’s Hospital in China

A Human Metapneumovirus Case Who Presented with Pertussis Like Illness

Identification of Etiological Agents of Pertussis-Like Syndrome in Children Younger Than 5 Years Old Hospitalized in Southwestern Iran

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