Detection of blaOXA-23 in Acinetobacter spp. isolated from patients of a university hospital

Corrêa, Laís Lisboa; Botelho, Larissa Alvarenga Batista; Barbosa, Livia C.; Mattos, Claudio Simões; Carballido, Jupira Miron; Castro, Carmem Lúcia Teixeira de; Mondino, Pedro Juan José; Paula, Geraldo Renato de; Mondino, Silvia Susana Bona de; Mendonça-Souza, Cláudia Rezende Vieira de

doi:10.1016/j.bjid.2012.10.003

Cited by 22 publications

(20 citation statements)

References 39 publications

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“…As long as therapeutic options for treating multidrug-resistant Acinetobacter spp. strains are quite limited, our results support the effectiveness of polymyxin B and tigecycline [37][38][39].…”

Section: Discussionsupporting

confidence: 81%

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Epidemiological characteristics and antimicrobial susceptibility among carbapenem-resistant non-fermenting bacteria in Brazil

Dias¹,

Diniz²,

Peter

et al. 2016

J Infect Dev Ctries

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Introduction: Non-fermenting Gram-negative bacteria such as Pseudomonas aeruginosa and Acinetobacter baumannii are widespread in the environment and are increasingly associated with nosocomial infections. Extensive and indiscriminate use of antibiotics in hospitals has contributed to an increased number of infections caused by these microorganisms, that are resistant to a wide variety of antimicrobials, including β-lactams. This study aimed to isolate and identify carbapenem-resistant Acinetobacter spp. and P. aeruginosa from hospitalized patients, to determine their antimicrobial susceptibility patterns and to screen for blaOXA , and blaOXA-143 genes among the isolated bacteria. Methodology: Antimicrobial resistance patterns were performed using the disk-diffusion method. Genetic markers related to carbapenem resistance were screened by polymerase chain reaction. Results: Carbapenem-resistant Acinetobacter spp. (n = 44) and P. aeruginosa (n = 28) samples were isolated from patients admitted to a tertiary hospital. Polymyxin B was the only effective drug for all isolates. Considering the oxacillinase gene screening, genetic markers were observed only in Acinetobacter isolates. The most frequent genotype observed was blaOXA-23 + /blaOXA-51 + (45.5%), followed by blaOXA-51 + /blaOXA-143

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Section: Discussionsupporting

confidence: 81%

“…Data from a national study carried out by others also corroborate these findings [38]. However, these data revealed a high frequency of isolates that were sensitive to tetracycline (approximately 85%), which were different from our results (44.4%).…”

Section: Discussionsupporting

confidence: 56%

Epidemiological characteristics and antimicrobial susceptibility among carbapenem-resistant non-fermenting bacteria in Brazil

Dias¹,

Diniz²,

Peter

et al. 2016

J Infect Dev Ctries

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“…Karbapenemi hidrolize eden ilk oksasilinaz olarak, 1985 yılında İngiltere'de tanımlanan OXA-23 enzimi, daha sonra hızla yayılmış ve OXA-23-benzeri enzimleri üreten izolatlar Avrupa, Asya ve Güney Amerika'da birçok merkezden bildirilmiştir [11][12][13][14][15] . Bizim çalışmamızda da, incelenen tüm suşlarda bla OXA-23 geninin varlığı saptanmış; bu durum hastanemizde OXA-23-benzeri enzimlerin karbapenem direncinden sorumlu önemli mekanizmalardan biri olduğunu düşündürmüştür.…”

Section: Discussionunclassified

Investigation of Oxacillinase Genes in Nosocomial Multidrug-Resistant Acinetobacter baumannii Isolates by Multiplex PCR and Evaluation of Their Clonal Relationship with Rep-PCR

Sarı¹,

Baran²,

Alaçam³

et al. 2015

Mikrobiyol Bul

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ÖZHastanede yatan hastalarda morbidite ve mortalitesi yüksek enfeksiyonlara yol açan Acinetobacter baumannii, önemli bir nozokomiyal patojendir. Birçok antibiyotik sınıfına, özellikle de karbapenemlere direnç geliştirmesi nedeniyle A.baumannii ciddi bir klinik problem haline gelmiştir. Bu çalışmanın amacı, çok ilaca dirençli (ÇİD) A.baumannii suşlarında karbapenem direncinden sorumlu oksasilinaz genlerinin araştırılması ve bu suşlar arasındaki klonal ilişkinin değerlendirilmesidir. Çalışmaya, Şubat-Mart 2012 tarihleri arasında hastanemizin yoğun bakım üniteleri (n= 42) ve diğer servislerinde (n= 20) yatan hastalara ait çeşitli klinik örneklerden (24 trakeal aspirat, 14 yara, 10 kan, 7 idrar, 2 apse, 2 balgam, 2 kateter ucu, 1 plevral sıvı) izole edilen toplam 62 ÇİD A.baumannii suşu dahil edilmiştir. İzolatlarının tanımlanma-sı ve antibiyotik duyarlılıklarının tespiti Vitek-2 otomatize sistemi (bioMérieux, Fransa) ile yapılmış; bakteri tanımlamasının doğrulanması için Maldi Biotyper (Bruker Daltonics, Almanya) sistemi kullanılmıştır. İmipenem, meropenem, kolistin ve tigesiklin duyarlılığı ek olarak E-test (bioMérieux, Fransa) ile değerlen-dirilmiştir. Karbapenemaz üretiminden sorumlu OXA tipi genlerin (bla OXA-23-like , bla OXA-40-like , bla OXA-51-like , Geliş Tarihi

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“…A similar report by Liakopoulos et al, [19] in Greece, noted that 120 out of 127 (95 %) A. baumannii isolates collected in 2011 were bla OXA-23 positive. Oxacillinase encoding gene bla OXA-23 was also reported as the most common carbapenamase encoding gene among Acinetobacter spp by Corrêa et al, [20] in Brazil and Santimaleeworagun et al, [15] in Thailand. It has been associated with resistance to carbapenems [21].…”

Section: Discussionmentioning

confidence: 99%

Genetic determinants of multi-drug resistance inAcinetobacter baumanniiat an academic hospital in Pretoria, South Africa

Nogbou

Phofa

Nchabeleng

et al. 2019

Preprint

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24Antimicrobial resistance is now globally recognised as the greatest threat to human 25 health. Acinetobacter baumanniis' (A. baumannii) clinical significance has been driven by its 26 ability to obtain and transmit antimicrobial resistance factors. In South Africa, A. baumannii 27 is a leading cause of healthcare associated infections (HAI). In this study, we investigated the 28 genetic determinants of multi-drug resistant A. baumannii (MDRAB) at a teaching hospital in 29 Pretoria, South Africa. 30One hundred non repetitive isolates of A. baumannii were collected for the study at Dr 31 George Mukhari Tertiary Laboratory (DGMTL). Antimicrobial susceptibility testing was 32 performed using the VITEK2 system (bioMerieux, France). The prevalence of common 33 resistance associated genes and AdeABC efflux pump system associated genes were 34 investigated using conventional PCR. Genetic relatedness of isolates was then determined 35 using rep-PCR. 36Seventy (70) of 100 isolates collected were confirmed to be multi-drug resistant and 37 were bla OXA51 positive. Phenotypically, the isolates where resistant to almost all tested 38 antibiotics. However, one isolate showed intermediate susceptibility to tigecycline while all 39 were susceptible to colistin. Oxacillinase encoding gene bla was the most detected at 40 99% and only 1% was positive for bla . The PCR results for metallo-betalactamase 41 (MBL) encoding genes showed that MBL bla VIM was the most frequently detected at 86% and 42 bla SIM-1 at 3% was the least detected. Out of 70 isolates, 56 isolates had the required gene 43 combination for an active efflux pump. The most prevalent clone was clone A at 69% of the 44 isolates. Regarding treatment; colistin and tigecycline are the most effective against strains 45 encountered at DGMTL as all tested carbapenems seem to have lost their effectiveness. 46The major genotypic determinants for drug resistances are oxacillinases: bla OXA-51 47 (100%) and bla (99%). The study reports for the first time, bla and bla SIM-1 48 detection in A. baumannii in South Africa. 49 50

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Detection of blaOXA-23 in Acinetobacter spp. isolated from patients of a university hospital

Abstract: OXA-23-like enzymes may be an important mechanism of carbapenem resistance among isolates present in the hospital studied.

Cited by 22 publications

References 39 publications

Epidemiological characteristics and antimicrobial susceptibility among carbapenem-resistant non-fermenting bacteria in Brazil

Epidemiological characteristics and antimicrobial susceptibility among carbapenem-resistant non-fermenting bacteria in Brazil

Investigation of Oxacillinase Genes in Nosocomial Multidrug-Resistant Acinetobacter baumannii Isolates by Multiplex PCR and Evaluation of Their Clonal Relationship with Rep-PCR

Genetic determinants of multi-drug resistance inAcinetobacter baumanniiat an academic hospital in Pretoria, South Africa

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