Detection of bla KPC-2 in Proteus mirabilis in Brazil

Cabral, Adriane Borges; Maciel, Maria Amélia Vieira; Barros, Josineide Ferreira; Antunes, Marcelo M.; Lópes, Ana Catarina de Souza

doi:10.1590/0037-8682-0152-2014

Cited by 14 publications

(5 citation statements)

References 14 publications

(16 reference statements)

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“…Further, the authors found that the bla KPC gene was detected in 80 % of the isolates, corroborating the data in this study, where we found an occurrence of 88.8 % for the bla KPC gene. These occurrence rates of the bla KPC gene identified in this study highlight the persistence of this gene in K. pneumoniae, and since its detection in 2006 from Recife, Brazil 13 , it remains the main carbapenemase associated with carbapenem-resistant K. pneumoniae samples in several Brazilian states, including Recife-PE 1,5,32 .…”

Section: Discussionsupporting

confidence: 56%

High plasmid variability, and the presence of IncFIB, IncQ, IncA/C, IncHI1B, and IncL/M in clinical isolates of Klebsiella pneumoniae with bla KPC and bla NDM from patients at a public hospital in Brazil.

Oliveira

Beltrão

Scavuzzi

et al. 2020

Rev. Soc. Bras. Med. Trop.

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Introduction: Antibiotic resistance in carbapenemase-producing Klebsiella pneumoniae is acquired and disseminated mainly by plasmids. Therefore, we aimed to investigate the occurrence of carbapenemase genes, analyze the genetic diversity by ERIC-PCR, and examine the most common plasmid incompatibility groups (Incs) in clinical isolates of K. pneumoniae from colonization and infection in patients from a hospital in Brazil. Methods: Twenty-seven isolates of carbapenem-resistant K. pneumoniae were selected and screened for the presence of carbapenemase genes and Incs by PCR, followed by amplicon sequencing. Results: The bla KPC and bla NDM genes were detected in 24 (88.8 %) and 16 (59.2 %) of the isolates, respectively. Thirteen isolates (48.1 %) were positive for both genes. The IncFIB (92.6 %) and IncQ (88.8 %) were the most frequent plasmids, followed by IncA/C, IncHI1B, and IncL/M, indicating that plasmid variability existed in these isolates. To our knowledge, this is the first report of IncHI1B in Brazil. We found eight isolates with clonal relationship distributed in different sectors of the hospital. Conclusions: The accumulation of resistance determinants, the variability of plasmid Incs, and the clonal dissemination detected in K. pneumoniae isolates demonstrate their potential for infection, colonization, and the dissemination of different resistance genes and plasmids.

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Section: Discussionsupporting

confidence: 56%

High plasmid variability, and the presence of IncFIB, IncQ, IncA/C, IncHI1B, and IncL/M in clinical isolates of Klebsiella pneumoniae with bla KPC and bla NDM from patients at a public hospital in Brazil.

Oliveira

Beltrão

Scavuzzi

et al. 2020

Rev. Soc. Bras. Med. Trop.

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“…However, studies have shown that P. mirabilis-CR strains are versatile in terms of carbapenemase production, MBL-NDM, VIMand IMP, 8,[34][35][36] or KPC-producing strains being frequently reported. 6,7,9 With regards to the P. rettgeri strain, this was confirmed to be MBL-NDM type. In general, these strains are rarely described in the literature and there are only a few studies conducted on larger groups.…”

Section: Discussionmentioning

confidence: 74%

“…5 The enzymes responsible for Proteeae resistance to carbapenems, which is considered to be of high epidemiological and clinical significance, are KPC, OXA-48 and NDM, VIM and IMP metallo-β-lactamases (MBL). [6][7][8][9][10][11][12][13] KPC is active on the classes of penicillins, cephalosporins, β-lactamase inhibitors and also on carbapenems and aztreonam, its activity being moderately inhibited by clavulanic acid. 3 MBLs have a significant hydrolytic activity on carbapenems, but without any effect on aztreonam, which they cannot hydrolyze.…”

Section: Introductionmentioning

confidence: 99%

Distribution of NDM1 Carbapenemase-Producing Proteeae Strains on High-Risk Hospital Wards

Rus¹,

Licker²,

Musuroi³

et al. 2020

IDR

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Background: Carbapenem-resistant Proteeae (CRP) is a group of multidrug-resistant (MDR) microorganisms that raise special treatment problems due to their intrinsic resistance to colistin. In this study, our aim is to provide a phenotypic and molecular characterization of the carbapenemases secreted by CRP strains isolated from inpatients from an intensive care unit (ICU) and surgical wards, as well as the identification of the risk factors involved in their acquisition. Methods: An observational, cross-sectional study was performed which included all Proteeae strains isolated in samples from inpatients on high-risk wards of the largest university hospital in Western Romania, from July 2017 to April 2019. Meropenemresistant strains (N=65) with MIC ≥16 µg/mL were subjected to a singleplex PCR assay for the detection of blaNDM, blaVIM and blaCTX-M genes. The analysis of risk factors was performed by logistic regression. Results: Out of 8317 samples that were processed, 400 Proteeae strains were isolated: 64% belonging to the genus Proteus, 26.75% to the genus Providencia and 9.25% to the genus Morganella. Most CRP strains (N=56) were of MBL type, and 55 had the blaNDM gene as the prevalent gene substrate. P. stuartii was the main species that provided the circulating MDR strains. Most CRP strains came from patients admitted to ICU, being isolated mainly from bronchial aspirates and blood cultures. Multivariate analysis revealed 3 independent risk factors -mechanical ventilation>96h ], p <0.001), tracheostomy (HR: 2.65 [1.14-6.17], p = 0.024) and prolonged antibiotic therapy (HR: 1.01 [1.00--1.02], p = 0.03). Conclusion:There is a significant increase in the incidence of CR P. stuartii strains, the MBL-blaNDM type being predominant. These strains presented various other resistance mechanisms, being often extremely difficult to treat and led to an excess of lethality of 27.16%.

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“…Out of 15 imipenem resistant Proteus spp., 20% isolates were positive for blaKPC gene. Single blaKPC-2 positive P. mirabilis was reported in the studies of Pilato et al (2016) and Cabral et al (2014), respectively.11,12 Possibility of this gene transmission may be due to horizontal transmission by transposons, the mobile genetic elements which can transfer from one bacterium to another. 13 Till now only a few numbers of NDM-1 producing P. mirabilis has been detected in di erent studies).…”

Section: Discussionmentioning

confidence: 99%

Emergence of Carbapenemase Encoding Genes in Proteus Species in Tertiary Care Hospital of Bangladesh

Afroz

Shamsuzzaman

Begum³

et al. 2021

Bangladesh Med J

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Along with different carbapenemase encoding genes, in recent year class D OXA enzymes are documented in Proteus spp which are not common in Enterobacteriaceae. The dissemination of plasmids, transposons and integrons among bacteria and species playing roles for this dissemination. So, this study was designed to observe the emergence and distribution of different classes of carbapenemase encoding genes among imipenem resistant Proteus spp. isolated from tertiary care hospital in Bangladesh. Total 15 imipenem resistant Proteus isolates were included in this study, which were collected from wound swab, pus, urine and blood samples. Identification was done by culture and biochemical test and antibiotic susceptibility test was done by disc diffusion method. MIC of imipenem (g/ml) was done among imipenem resistant P. mirabilis by agar dilution method. blaKPC, blaNDM-1, blaVIM, blaIMP, blaOXA-48 like, blaOXA-23 like, blaOXA-51 like, blaOXA-58 like carbapenemase encoding genes were detected among imipenem resistant Proteus spp. by PCR and sequencing of blaOXA-48 like, blaOXA-51 like gene done by capillary method to compare the sequences with the same gene, available in gene bank. Among 15 imipenem resistant isolates blaNDM-1 (26.67%), blaKPC (20%), blaVIM (20%), blaOXA-484 (20%) were predominant carbapenemase encoding genes followed by blaOXA-66(6.67%). This study finds that blaOXA-484 gene and blaOXA-66 class D carbapenemase encoding genes are emerging in Proteus spp. and may play a contributing factor in developing carbapenem resistance. Bangladesh Med J. 2020 May; 49(2) : 1-8

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Detection of bla KPC-2 in Proteus mirabilis in Brazil

Cited by 14 publications

References 14 publications

High plasmid variability, and the presence of IncFIB, IncQ, IncA/C, IncHI1B, and IncL/M in clinical isolates of Klebsiella pneumoniae with bla KPC and bla NDM from patients at a public hospital in Brazil.

High plasmid variability, and the presence of IncFIB, IncQ, IncA/C, IncHI1B, and IncL/M in clinical isolates of Klebsiella pneumoniae with bla KPC and bla NDM from patients at a public hospital in Brazil.

Distribution of NDM1 Carbapenemase-Producing Proteeae Strains on High-Risk Hospital Wards

Emergence of Carbapenemase Encoding Genes in Proteus Species in Tertiary Care Hospital of Bangladesh

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