Detection of beryllium in digested autopsy tissues by inductively coupled plasma mass spectrometry using a high matrix interface configuration

Abstract

This article describes a robust methodology using the combination of instrumental design (high matrix interface-HMI), sample dilution and internal standardization for the quantification of beryllium (Be) in various digested autopsy tissues using inductively coupled plasma mass spectrometry. The applicability of rhodium as a proper internal standard for Be was demonstrated in three types of biological matrices (i.e., femur, hair, lung tissues). Using HMI, it was possible to achieve instrumental detection limits and sensitivity of 0.6 ng L(-1) and 157 cps L ng(-1), respectively. Resilience to high salt matrices of the HMI setup was also highlighted using bone mimicking solution ([Ca(2+)] = 26 to 1,400 mg L(-1)), providing a 14-fold increase in tolerance and a 2.7-fold decrease in method detection limit compared to optimized experimental conditions obtained without the HMI configuration. Precision of the methodology to detect low levels of Be in autopsy samples was demonstrated using hair and blood certified reference materials. Be concentration ranging from 0.015 to 255 μg kg(-1) in autopsy samples obtained from the U.S. Transuranium and Uranium Registries were measured using the methodology presented.

Fabrication of Anchored Complexes as Electrodes for Sensing Heavy Metal Ions by Electrochemical Method

“…Beryllium is a deceptive cancer-causing poison because of its trouble in biodegradation 47 .It can be recognized by means of atomic absorption and emission spectrometry 48 and fluoro spectrometry 49 . In any case, techniques either lack affectability and selectivity or need costly mechanical assembly and entangled investigation.…”

Atomic spectrometry update. Clinical and biological materials, foods and beverages

“…Calibration was achieved using a dosing frequency-based technique and could be applied for external calibration or standard addition in the case of complex matrices. Lariviere et al 43 described the use of a high matrix interface system for the measurement of Be in autopsy tissues. Aer optimisation, the instrumental LOD was 0.6 ng L À1 despite the high level of salt present in digests from samples such as bone or hair.…”

“…The quantitative determination of trace concentrations of Be in biological matrices using ICP-MS oen involves some form of sample treatment to pre-concentrate Be from the sample matrix prior to measurement of the element. In an effort to avoid the need for element pre-concentration, Lariviere et al 43 combined a high matrix interface conguration with internal standardisation to quantitatively determine Be in diluted samples of human autopsy tissue digests. Optimisation of the interface conguration enabled the system to deal with the high salt and dissolved solids within the digested samples and gave a reported 2.7-fold improvement in LOD (0.6 mg L À1 ).…”

Fit-for-Purpose ICP-MS Procedure Supporting Brazilian Regulations on Inorganic Constituents in Fresh and Saline Waters

“…[7][8][9] Additionally, high matrix introduction (HMI) technology uses auto-optimization of aerosol dilution to further improve matrix tolerance, reducing both aerosol density and water vapor loading in the plasma. 8,[10][11][12][13][14][15][16] Wilbur and Jones 8 demonstrated the benefits of HMI system for determination of 26 elements in complex matrix certified reference materials (CRM), such as river sediment and soil samples. Aqueous samples were investigated using fast protein liquid chromatography (FPLC) coupled to ICP-MS with a high matrix introduction system.…”