Detection of bacterial contamination of platelet concentrates

Pietersz, R. N. I.; Engelfriet, C. P.; Reesink, H. W.; Wood, Erica M.; Winzar, S.; Keller, A. J.; Wilson, John T.; Henn, Guilherme Alves de Lima; Mayr, W. R.; Ramirez‐Arcos, Sandra; Goldman, Mindy; Georgsen, Jørgen; Morel, Pascal; Hervé, P.; Andeu, G.; Assal, Azzedine; Seifried, Erhard; Schmidt, Michael; Foley, M.; Doherty, C.; Coakley, P.; Salami, Ayodeji E.; Cadden, E.; Murphy, William G.; Satake, Masahiro; Korte, Dirk de; Bosnes, Vidar; Kjeldsen‐Kragh, Jens; McDonald, Carl; Brecher, Mark E.; Yomtovían, Roslyn; AuBuchon, James P.

doi:10.1111/j.1423-0410.2007.00967.x

Cited by 48 publications

(55 citation statements)

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“…Only in the USA most of the whole blood derived platelets are screened by measuring the pH or glucose or other rapid methods. These tests had 4-6 folds lower detection rate than the culture based methods [13].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Sensitivity and Specificity of Use of Glucose and pH for Bacterial Screening of Platelet Concentrates Compared to the Bact/Alert

Razjou

Naghadeh

Ferdowsi

et al. 2016

Indian J Hematol Blood Transfus

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Bacterial contamination of blood components is the major infectious risk in transfusion medicine. Since platelets should be stored at room temperature that makes them an excellent growth medium for bacteria; it is mentioned as a major problem in transfusion medicine. Transfusion risk of a bacterial contaminated platelet concentrate is higher than viral pathogen such as HIV, HBV, HCV and HTLV. The objective of this study was to evaluation of the sensitivity and specificity of use of glucose and pH for bacterial screening of platelet concentrates compared to the Bact/Alert. 1332 platelet concentrates were screened by the Bact/Alert system for aerobic and anaerobic bacterial contamination. Bacterial contamination was also evaluated by using urine reagent strips (Multistix10 SG Bayer) and culture methods. Moreover PH screening with a pH meter (Metrohm 744 Swiss) and glucose was also used for detection of bacterial contamination. The rate of bacterial contamination detected by the Bact/Alert system in platelet concentrates was 25 in 1332 (1.9 %). It contained 15 (1.1 %) for aerobic bacteria and 10 (.8 %) for anaerobic bacteria. 226 of 1332 were considered as containing bacteria by using urine reagent strips. Six of the 226 units were also positive by the Bact/Alert system. Three of those units were culture positive for aerobic bacteria and three for anaerobic. The result of platelet concentrates that underwent pH screening by use of pH meter and a pH portion of urine reagent strips was the same. The sensitivity and specificity of considering glucose alone for detection of bacterial contamination were 12 and 98 % respectively. For pH alone, these were 24 and 83 %. For glucose and/or pH, these were 24 and 83 %; and for combination of glucose and pH, these were 12 and 98 %. Our results showed use of glucose/pH strips would improve the safety of blood products and should be encouraged.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the Sensitivity and Specificity of Use of Glucose and pH for Bacterial Screening of Platelet Concentrates Compared to the Bact/Alert

Razjou

Naghadeh

Ferdowsi

et al. 2016

Indian J Hematol Blood Transfus

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“…In 2007, the International Society of Blood Transfusion (ISBT) held an international forum [8], which reported on bacterial detection in platelet concentrates in 12 countries. Eight of these 12 countries used BacT/ ALERT (bioMérieux, Nürtingen, Germany).…”

Section: Culture Methods In Combination With a 'Negative-to-date' Conmentioning

confidence: 99%

Implementation of Bacterial Detection Methods into Blood Donor Screening – Overview of Different Technologies

Schmidt

Sireis

Seifried³

2011

Transfus Med Hemother

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Background: Through the implementation of modern technology, such as nucleic acid testing, over the last two decades, blood safety has improved considerably in that the risk of viral infection is less than 1 in a million blood transfusions. By contrast, the residual risk of transfusion-associated bacterial infection is stable at approximately 1 in 2,000 to 1 in 3,000 in platelets. To improve blood safety with regard to bacterial infections, many countries have implemented bacterial screening methods as part of their blood donor screening programmes. Methods: Bacterial detection methods are clustered into three groups: i) culture methods in combination with the ‘negative-to-date’ concept, ii) rapid detection systems with a late sample collection, and iii) bedside screening tests. Results: The culture methods are convincing because of their very high analytical sensitivity. Nevertheless, false-negative culture results and subsequent fatalities were reported in several countries. Rapid bacterial systems are characterised as having short testing time but reduced sensitivity. Sample errors are prevented by late sample collection. Finally, bedside tests reduce the risk for sample errors to a minimum, but testing outside of blood donation services may have risks for general testing failures. Conclusion: Bacterial screening of blood products, especially platelets, can be performed using a broad range of technologies. Each system exhibits advantages and disadvantages and offers only a temporary solution until a general pathogen inactivation technology is available for all blood components.

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“…In the meantime, automated bacterial culture systems are used in some countries to screen PCs for bacterial contamination [6,7]. BacT/ALERT™ (bioMérieux, Durham, NC, USA) is the system most commonly employed [8]. Product samples obtained near to the time of preparation of the PCs are transferred to culture bottles and incubated.…”

Section: Introductionmentioning

confidence: 99%

Screening of platelet concentrates for bacterial contamination: spectrum of bacteria detected, proportionof transfused units, and clinical follow-up

et al. 2009

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Detection of bacterial contamination of platelet concentrates

Cited by 48 publications

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Evaluation of the Sensitivity and Specificity of Use of Glucose and pH for Bacterial Screening of Platelet Concentrates Compared to the Bact/Alert

Evaluation of the Sensitivity and Specificity of Use of Glucose and pH for Bacterial Screening of Platelet Concentrates Compared to the Bact/Alert

Implementation of Bacterial Detection Methods into Blood Donor Screening – Overview of Different Technologies

Screening of platelet concentrates for bacterial contamination: spectrum of bacteria detected, proportionof transfused units, and clinical follow-up

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