Detection of Babesia caballi and Babesia equi in Dermacentor nuttalli adult ticks

Battsetseg, Badgar; Xuan, Xuenan; Ikadai, Hiromi; Rodríguez-Bautista, José Luis; Byambaa, Badarch; Boldbaatar, Damdinsuren; Battur, Banzragch; Gonchigoo, Battsetseg; Batsukh, Zayat; Igarashi, Ikuo; Nagasawa, Hideyuki; Mikami, Takeshi; Fujisaki, Kozo

doi:10.1016/s0020-7519(01)00120-5

Cited by 71 publications

(42 citation statements)

References 6 publications

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“…Twenty-one percent of questing Ixodes ricinus ticks were PCR positive for Babesia sp., which correlates with infection rates described in other studies: between 6.2% and 62% of babesial infection in questing [3,15], or engorged ticks [17]. Although the particular species of Babesia was not determined by sequencing, we confirmed that the amplification was specific to Babesia sp.…”

Section: Discussionsupporting

confidence: 90%

Evidence of Bartonella sp. in questing adult and nymphal Ixodes ricinus ticks from France and co-infection with Borrelia burgdorferi sensu lato and Babesia sp.

et al. 2005

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-Ticks are known vectors for a wide range of pathogenic microorganisms. Their role in the transmission of some others is so far only suspected. Ticks can transmit multiple pathogens, however, little is known about the co-existence of these pathogens within questing ticks. We looked for the presence of DNA from three micro-organisms, Bartonella sp., Borrelia burgdorferi sensu lato and Babesia sp. which are known or suspected tick-borne pathogens, using a cohort of 92 questing Ixodes ricinus ticks collected from pastures in northern France. DNA was extracted from each individual tick and the presence of the three pathogens was investigated using Polymerase Chain Reaction (PCR) amplification. Nine among 92 samples (9.8%) demonstrated PCR products using Bartonella specific primers, 3 among 92 (3.3%) using Borrelia burgdorferi sensu lato specific primers and 19 among 92 (20.6%) using Babesia specific primers. Seven among 92 samples (7.6%) were PCR positive for at least two of the pathogens and one sample was positive for all three. Adult ticks (12/18; 67%) showed significantly higher infection rates compared to nymphs (11/74; 15%) for all three pathogens (P < 0.001). This study is the demonstration of the simultaneous presence of Bartonella sp., Borrelia burgdorferi sensu lato and Babesia sp. in questing Ixodes ricinus ticks. questing ticks / co-infection / PCR detection

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Section: Discussionsupporting

confidence: 90%

Evidence of Bartonella sp. in questing adult and nymphal Ixodes ricinus ticks from France and co-infection with Borrelia burgdorferi sensu lato and Babesia sp.

et al. 2005

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“…Each sample of extracted DNA was used as template in nested PCR assays, using previously described specific protocols based on ema-1 gene for T. equi (NICOLAIEWSKY et al, 2001) and rap-1 gene for B. caballi (IKADAI et al, 1999;BATTSETSEG et al, 2001). Briefly, five microliters of DNA was used as a template in 25 µL reaction mixtures containing 10X PCR buffer, 1.0 mM MgCl 2 , 0.6 mM deoxynucleotide triphosphate (dNTPs) mixture, 1.5 U of Taq DNA polymerase (Life Technologies) and 0.5 µM each primer (Integrated DNA Technologies) ( Table 1).…”

Section: Molecular Detection Of Equid Piroplasmidsmentioning

confidence: 99%

Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil

Braga

Costa

Gomes

et al. 2017

Rev. Bras. Parasitol. Vet.

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Equine piroplasmosisis, a tick-borne disease caused by the intra-erythrocytic protozoans Babesia caballi and Theileria equi, has economic importance due to the international trade and the increased movement of horses all over the world. The goal of this study was to evaluate the occurrence of phylogenetic diversity of T. equi and B. caballi genotypes among infected equids from São Luís Island, state of Maranhão, northeastern Brazil. Between December of 2011 and June of 2012, EDTA-blood and serum samples were collected from 139 equids (90 donkeys, 39 horses and 10 mules). From 139 serum samples submitted to ELISA assay, IgG antibodies to T. equi and B. caballi were detected in 19.4% (27/139) and 25.2% (35/139), respectively. Among sampled animals, 21.6% (30/139) and 55.4% (77/139) were positive for cPCR assays for T. equi and B. caballi, based on ema-1 and rap-1 genes, respectively. Overall, the T. equi sequences (n=7) submitted to Maximum Likelihood analysis (based on a 18S rRNA fragment of 1700 bp after alignment) grouped into three main groups, which were subdivided in eight clusters. The present work showed that different genotypes of T. equi and B. caballi circulate among equids in Brazil.Keywords: Babesia caballi, Theileria equi, phylogeny, 18S rRNA. ResumoA piroplasmose equina, uma doença transmitida por carrapatos e causada pelos protozoários intra-eritrocíticos Babesia caballi e Theileria equi, tem importância econômica devido ao comércio internacional e ao aumento do movimento de cavalos em todo o mundo. O objetivo do presente estudo foi mostrar a diversidade filogenética de T. equi e B. caballi infectando cavalos, burros e jumentos na Ilha de São Luís, Estado do Maranhão, Nordeste do Brasil. Entre dezembro de 2011 e junho de 2012, amostras de sangue com EDTA e soro de foram coletadas de 139 equídeos (90 jumentos, 39 cavalos e 10 burros). Dentre as 139 amostras de soro submetidas ao ensaio de ELISA, foram detectados anticorpos IgG contra T. equi e B. caballi em 19,4% (27/139) e 25,2% (35/139), respectivamente. Entre os animais amostrados, 21,6% (30/139) e 55,4% (77/139) foram positivos por meio dos ensaios de cPCR para T. equi e B. caballi, com base nos genes ema-1 e rap-1, respectivamente. No geral, as sequências T. equi (n = 7) submetidas à análise de Máxima Verossimilhança (baseada em um fragmento do 18S rRNA de 1700 pb, após o alinhamento) foram agrupadas em três grupos principais, os quais foram subdivididos em oito grupos. O presente trabalho mostrou que diferentes genótipos de T. equi e B. caballi circulam entre equídeos no Brasil.Palavras-chave: Babesia caballi, Theileria equi, filogenia, 18S rRNA.

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“…Although tick infestation with Dermacentor nuttalli, Dermacentor silvarum and Hyalomma dromedari is common in Mongolian horses [6][7], scientific information on tick transmission is wanting. Battsetseg et al [4], have recently reported the amplification of specific-equine Babesia parasite gene fragments in field-collected (i.e. nonengorged) D. nuttalli ticks in Mongolia, suggesting its vectorial role in Babesia parasite transmission.…”

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confidence: 99%

Detection of Equine Babesia spp. Gene Fragments in Dermacentor nuttalli Olenev 1929 Infesting Mongolian Horses, and Their Amplification in Egg and Larval Progenies.

Battsetseg

Lucero

Xuan

et al. 2002

The Journal of Veterinary Medical Science

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ABSTRACT. Babesia equi (EMA-1) and Babesia caballi (BC48) gene fragments were amplified by polymerase chain reaction (PCR), in blood samples, and partially fed-females and egg and larval progenies of Dermacentor nuttalli, collected from horses in Altanbulag, Tuv Province, Mongolia. While Babesia parasite DNA was detected in some horse blood samples during the first PCR, all positive cases in partially fed-female ticks, eggs and larvae were confirmed by nested PCR. Present study reinforces earlier similar findings in unfed D. nuttalli ticks collected from an open space vegetation in Bayanonjuul, Tuv Province in Central Mongolia, pointing to the most likely important role of D. nuttalli in the transmission of equine babesiosis in Mongolia. The detection of parasite DNA in eggs and larval progenies is likewise suggestive of transovarial parasite transmission in this tick species.

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Detection of Babesia caballi and Babesia equi in Dermacentor nuttalli adult ticks

Cited by 71 publications

References 6 publications

Evidence of Bartonella sp. in questing adult and nymphal Ixodes ricinus ticks from France and co-infection with Borrelia burgdorferi sensu lato and Babesia sp.

Evidence of Bartonella sp. in questing adult and nymphal Ixodes ricinus ticks from France and co-infection with Borrelia burgdorferi sensu lato and Babesia sp.

Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil

Detection of Equine Babesia spp. Gene Fragments in Dermacentor nuttalli Olenev 1929 Infesting Mongolian Horses, and Their Amplification in Egg and Larval Progenies.

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