Detection of Babesia bovis in blood samples and its effect on the hematological and serum biochemical profile in large ruminants from Southern Punjab

Zulfiqar, Samreen; Shahnawaz, Sadia; Ali, Muhammad Arif; Bhutta, Arif Mahmood; Iqbal, Shahid; Hayat, Sikandar; Qadir, Shazia; Lateef, Muhammad; Kiran, Nazia; Saeed, Ali; Iqbal, Furhan

doi:10.1016/s2221-1691(11)60202-5

Cited by 41 publications

(36 citation statements)

References 27 publications

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“…[23] and Zulfiqar et al . [24] reported greater sensitivity and specificity of PCR assays over the existing tests for diagnosis of bovine babesiosis.…”

Section: Discussionmentioning

confidence: 99%

Diagnosis and management of bovine babesiosis outbreaks in cattle in Punjab state

Bal¹,

Mahajan²,

Filia³

et al. 2016

Vet World

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Aim:The aim of the present study was to diagnose severe outbreaks of bovine babesiosis in Punjab state, in the year 2015 and to suggest control and preventive measures to animal owners.Materials and Methods:Mortality of animals was recorded in two cattle herd comprising a total of 465 cattle in Sangrur (n=125) and Faridkot (n=340) districts. There was a history of purchase of animals at one farm. 23 blood samples were collected from diseased (n=15) and healthy animals (n=8) for hematological analysis, parasitological, and polymerase chain reaction (PCR)-based diagnosis. Ticks were also collected from animals for identification.Results:Out of 465 cattle at risk, 28 were critically ill and 14 died of disease with morbidity, mortality, and case fatality rate of 6.02%, 3.01%, and 50.00%, respectively. Clinical signs and necropsy findings were suggestive of babesiosis. Ticks collected from both the outbreaks were identified as Rhipicephalus (Boophilus) microplus. Thin blood smears from infected animals (especially with clinical sign of hemoglobinuria) were found positive for Babesia bigemina organisms; however, molecular diagnosis (PCR) further confirmed the disease. Animals were successfully treated with diminazene aceturate, hematinics, and antipyretics.Conclusions:Two fatal outbreaks of babesiosis in cattle were diagnosed with application of conventional parasitological, hematological, and molecular diagnostic techniques. PCR was found to be far more sensitive in detecting the disease, especially in latent infections. Animal owners were advised to follow quarantine measures before mixing new animals in the herd and strategic acaricidal treatments for effective tick control.

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“…[23] and Zulfiqar et al . [24] reported greater sensitivity and specificity of PCR assays over the existing tests for diagnosis of bovine babesiosis.…”

Section: Discussionmentioning

confidence: 99%

Diagnosis and management of bovine babesiosis outbreaks in cattle in Punjab state

Bal¹,

Mahajan²,

Filia³

et al. 2016

Vet World

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“…Inorganic method of DNA extraction was used following Iqbal et al (2011) and Zulfiqar et al (2012). The quality of the DNA extract in regard to purity and integrity was assessed with optical density counts at 260/280 nm and submerged gel electrophoresis.…”

Section: Methodsmentioning

confidence: 99%

A comparison of two different techniques for the detection of blood parasite,Theileria annulata, in cattle from two districts in Khyber Pukhtoon Khwa Province (Pakistan)

et al. 2012

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The present study was carried out to determine the prevalence of Theileria annulata in large ruminants from two districts, Peshawar and Kohat, in Khyber Pukhtoon Khwa (Pakistan). Blood samples were collected from 95 cattle. Data on the characteristics of animals and herds were collected through questionnaires. No significant risk factors were found associated with the spread of tropical theileriosis in the study area. Two different parasite detection techniques, PCR amplification and screening of Giemsa stained slides, were compared and it was found that PCR amplification is a more sensitive tool (33.7% parasite detection), as compared to smear scanning (5.2% parasite detection) for the detection of Theileria annulata. 32 out of 95 animals, from both districts, produced the 721-bp fragment specific for Theileria annulata.

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“…Blood was collected from the jugular vein of the animals by using disposable syringe with needle. The collected blood was immediately preserved in 10 (Korea) and by using kits manufactured by Randox LTD Laboratories (UK) following Shahnawaz et al [7] , Zulfiqar et al [8] and Khan et al [9] .…”

Section: Methodsmentioning

confidence: 99%

Effect of age and gender on some blood biochemical parameters of apparently healthy small ruminants from Southern Punjab in Pakistan

Kiran

Bhutta²,

Khan

et al. 2012

Asian Pacific Journal of Tropical Biomedicine

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Detection of Babesia bovis in blood samples and its effect on the hematological and serum biochemical profile in large ruminants from Southern Punjab

Abstract: : This study has reported for the first time the presence of B. bovis in large ruminant and the results can lead to the prevention of babesiosis in the region to increase the livestock output.

Cited by 41 publications

References 27 publications

Diagnosis and management of bovine babesiosis outbreaks in cattle in Punjab state

Diagnosis and management of bovine babesiosis outbreaks in cattle in Punjab state

A comparison of two different techniques for the detection of blood parasite,Theileria annulata, in cattle from two districts in Khyber Pukhtoon Khwa Province (Pakistan)

Effect of age and gender on some blood biochemical parameters of apparently healthy small ruminants from Southern Punjab in Pakistan

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