A comparison of two different techniques for the detection of blood parasite,<i>Theileria annulata</i>, in cattle from two districts in Khyber Pukhtoon Khwa Province (Pakistan)

Khattak, Rehman Mehmood; Rabib, M.; Khan, Zaheer; Ishaq, Mohd; Hameed, Hafsa; Taqddus, Asia; Faryal, M.; Durranis, S.; Gillani, Quratulane; Allahyar, Razia; Shaikh, Rehan Sadiq; Khan, M. A.; Ali, Muhammad Arif; Iqbal, Furhan

doi:10.1051/parasite/2012191091

Cited by 27 publications

(35 citation statements)

References 14 publications

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“…Higher prevalence of T. annulata found in females is similar to previous reports [29,33] . There is only one report, from Egypt, of higher prevalence of T. annulata in males than in females [34] .…”

Section: Discussionsupporting

confidence: 92%

“…This is in accord with earlier reports that stained smear blood examination cannot detect all subclinical or chronic infections, because parasitemia is often extremely low and may be missed [4,27] . Several reports have clearly demonstrated that PCR is a more sensitive and specific test than the conventional thin blood smear [28][29][30] and is reliable for detecting early or carrier infections.…”

Section: Discussionmentioning

confidence: 99%

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Pakistan’ın Cholistan Çölünde Başıboş Dolaşan Cholistan Sığırlarında Tropikal Theileriosisin Prevalansı ve Kan Değerleri

SAEED¹,

Shaikh²,

GULSHER³

et al. 2016

Kafkas Univ Vet Fak Derg

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This is the first report on tropical theileriosis in Cholistani cattle, with the aim of 1) assessing the reliability of PCR as a tool for diagnosis of the early/carrier state; 2) determining the prevalence of theileriosis; and 3) comparing haematological profiles of parasite-positive and parasite-negative cattle. A total of 264 cattle (142 female and 122 male; 127 adult and 137 young) were examined for tropical theileriosis through clinical examination, stained smear screening, and polymerase chain recation. No cattle showed clinical signs of the disease. Of the diagnostic tests, PCR was more sensitive for detection of the early/carrier state of theileriosis (19.3%) compared to stained thin blood smear examination (1.9%). Female (24.6%) and young animals (23.4%) showed higher prevalence than males and adults, but not significant (P>0.05). Prevalence of the disease (51.6%) was significantly higher (P<0.05) in summer. Haematological indices were not significantly different in parasite-positive compared to parasite-negative cattle, except for total protein and creatinine which were significantly higher in infected animals. The study revealed a substantial prevalence of tropical theileriosis in Cholistani cattle. Nevertheless, their adaptation to the climate and their potential for tick and disease resistance may reflect in the absence of clinical signs and in normal haematological indices. Keywords: Theileria annulata, Cattle, PCR, Pakistan Pakistan'ın Cholistan Çölünde Başıboş Dolaşan Cholistan Sığırlarında Tropikal Theileriosisin Prevalansı ve Kan Değerleri ÖzetCholistan sığırlarında tropical theileriosis ile ilgili ilk rapor olan bu çalışma ile; 1 teşhis yöntemi olarak taşıyıcı hayvanlarda PCR metodunun güvenirliliğinin belirlenmesi; theileriosisin prevalansının tespit edilmesi; parazit pozitif ve negative bireylerde kan parametrelerinin karşılaştırılması hedeflenmiştir. Toplam 264 sığır klinik, mikroskopik ve PCR ile tropical theileriosis yönünden muayene edilmiştir. Sığırların hiç birinde klinik bulgu gözlenmemiştir. PCR (%19.3), subklinik enfeksiyonların belirlenmesinde mikroskopik bakıya (%1.9) göre oldukça duyarlı bulunmuştur. Hastalğın yaygınlığı dişi (24.6%) ve genç hayvanlarda (%23.4) erkek ve erişkinlere göre daha yüksek bulunmuş, ancak bu farklılığın istatistiksel olarak anlamlı olmadığı (P>0.05) görülmüştür. Hastalığın, yaz aylarında (%51.6) daha yüksek olduğu tespit edilmiştir. T. annulata yönünden pozitif ve negative hayvanların kan değerlerinde total protein ve creatinine hariç (bu değerler enfekte hanvanlarda yüksek bulunmuştur) bir farklılık gözlenmemiştir. Bu çalışma Cholistan sığırlarında hastalığın prevalansının yüksek olduğunu ortaya koymuştur. Muayene edilen hayvanların hiç birinde klinik bulguların gözlenmemesi, kan değerlerinde değişikliğin olmaması, bu sığırların kenelere ve hastalığa karşı dirençlerini yansıtmaktadır. Anahtar sözcükler: Theileria annulata,

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Pakistan’ın Cholistan Çölünde Başıboş Dolaşan Cholistan Sığırlarında Tropikal Theileriosisin Prevalansı ve Kan Değerleri

SAEED¹,

Shaikh²,

GULSHER³

et al. 2016

Kafkas Univ Vet Fak Derg

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“…Ticks were found on 18.52% of the infected animals suggesting that ticks were the vector for this parasite although this correlation was statistically significant in ULRM. A similar trend of tick infestation and theileriosis was previously reported by (Rehman et al, 2004;Ghosh et al, 2007 andKhattak et al, 2012).…”

Section: Presence Of Ticksupporting

confidence: 89%

Study of prevalence and associated risk factors of anaplasmosis and theileriasis in cattle

Kispotta

Islam

Hoque

et al. 2017

Asian J. Med. Biol. Res

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Abstract:A detailed cross-sectional study was carried out to investigate the prevalence and associated risk factors of theileriosis and anaplasmosis in cattle of Dinajpur district over a period of six months from January to June 2014. A total of 200 samples were randomly collected from the cattle. A pretested questionnaire was implemented to collect data on different risk factors. Blood smears were prepared and examined under microscope (100 xs) with Giemsa's stain. The study revealed proportional prevalence was 10% for theileriasis and 18.5% for anaplasmosis. The percentages of theileriasis and anaplasmosis 12.61% and 22.52% were higher in female than in male animal 6.74% and 13.48% respectively. In both cases, female showed higher positivity in compare with male. The univariate logistic regression analysis revealed cattle with a herd size >10 (OR=4.3), temperature (≥ 103ºF) (OR=3.27), presence of tick in the body surface (OR=3.09) and record of Ivermectin treatment (OR=4.47) were found significantly associated with theileriasis prevalence (p<0.05) and irregular bathing history was found significantly associated in the case of anaplasmosis prevalence (OR=0.23). However, in multivariate analysis, temperature (≥ 103ºF) (OR=2.89), presence of tick in the body surface (OR=2.80) and animal with a record of Ivermectin treatment (OR=4.24) showed significant association with theileriasis and herd size >10, deep brown; white colour and bathing history were significantly associated (P < 0.05) with the anaplasmosis. Good husbandry practice and adoption of hygienic measures are needed among the high risk group or individuals to minimize the spread of the disease.

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“…Kamani et al (2010) observed that the higher prevalence was found in adult than young cattle. Sarkar (2007) and Khattak et al (2012) also found that the higher prevalence was showed in female than male. Prevalence of the disease was higher in croosbreed cattle than local cattle which was reported by Siddiki et al (2010) and Esin et al (2012).…”

Section: Introductionmentioning

confidence: 88%

Prevalence of theileriosis and babesiosis in cattle in Sirajganj district of Bangladesh

Mahmud¹,

Belal²,

Hossain

2015

Res. Agric. Livest. Fish.

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A study was conducted to investigate the prevalence of theileriosis and babesiosis in cattle in Sirajganj district of Bangladesh during the period of December 2013 to November 2014. During one year study period a total of 395 cattle were examined, 23 and 8 were found to be infected with Theileria spp. and Babesia spp., respectively. On Geimsa stained blood smear examination, it was observed that the overall prevalence of theileriosis and babesiosis in cattle were recorded as 5.82% and 2.27% respectively. The effect of age, sex, breed and season was observed in cattle during this study. The highest prevalence of theileriosis (7.25%) and babesiosis (3.10%) was reported in the older cattle (>3 years of age) and the higher prevalence was observed in female (6.66% and 2.59%, respectively) than male (4.0% and 1.60% respectively). All crossbred cattle was showed higher prevalence than local cattle. The prevalence of theileriosis was noticed as the highest in the rainy season (6.25%) in relation to summer (5.83%) and winter (5.05%) season. But the prevalence of babesiosis was ranked the highest in summer season (2.50%) followed in rainy (2.27%) and winter (2.02%) season that was insignificant. From the study it was evident that cattle were infected with the organisms and caused a heavy economic loss which will assist to take necessary preventive measurements.Res. Agric., Livest. Fish.2(1): 79-86, April 2015

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A comparison of two different techniques for the detection of blood parasite,Theileria annulata, in cattle from two districts in Khyber Pukhtoon Khwa Province (Pakistan)

Cited by 27 publications

References 14 publications

Pakistan’ın Cholistan Çölünde Başıboş Dolaşan Cholistan Sığırlarında Tropikal Theileriosisin Prevalansı ve Kan Değerleri

Pakistan’ın Cholistan Çölünde Başıboş Dolaşan Cholistan Sığırlarında Tropikal Theileriosisin Prevalansı ve Kan Değerleri

Study of prevalence and associated risk factors of anaplasmosis and theileriasis in cattle

Prevalence of theileriosis and babesiosis in cattle in Sirajganj district of Bangladesh

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