Detection of association of IL1β, IL4R, and IL6 gene polymorphisms with cervical cancer in the Bangladeshi women by tetra-primer ARMS-PCR method

Muhammad, Sayma Binte; Hassan, Fahomida; Bhowmik, Khokon Kanti; Millat, Md. Shalahuddin; Sarwar, Shahid; Aziz, Abdul; Barek, Md. Abdul; Uddin, Mohammad Sarowar; Ferdous, Mahmuda; Islam, Mohammad Safiqul

doi:10.1016/j.intimp.2020.107131

Cited by 16 publications

(11 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some research argued that IL1A and IL1B were associated with tumor progression and prognosis; in addition, IL-1A was an independent predictor of survival in cervical cancer patients. [ 47 – 49 ]. Furthermore, IL1B could be predicted the risk of breast cancer patients developing bone metastases [ 50 , 51 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of a necroptosis-related prognostic gene signature associated with tumor immune microenvironment in cervical carcinoma and experimental verification

Sun

Huang

et al. 2022

World J Surg Onc

View full text Add to dashboard Cite

Cervical carcinoma (CC) has been associated with high morbidity, poor prognosis, and high intratumor heterogeneity. Necroptosis is the significant cellular signal pathway in tumors which may overcome tumor cells’ apoptosis resistance. To investigate the relationship between CC and necroptosis, we established a prognostic model based on necroptosis-related genes for predicting the overall survival (OS) of CC patients. The gene expression data and clinical information of cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) patients were obtained from The Cancer Genome Atlas (TCGA). We identified 43 differentially expressed necroptosis-related genes (NRGs) in CESC by examining differential gene expression between CESC tumors and normal tissues, and 159 NRGs from the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Gene ontology (GO) and KEGG enrichment analysis illustrated that the genes identified were mainly related to cell necrosis, extrinsic apoptosis, Influenza A, I − kappaB kinase/NF − kappaB, NOD − like receptor, and other signaling pathways. Subsequently, least absolute shrinkage and selection operator (LASSO) regression and univariate and multivariate Cox regression analyses were used to screen for NRGs that were correlated with patient prognosis. A prognostic signature that includes CAMK2A, CYBB, IL1A, IL1B, SLC25A5, and TICAM2 was established. Based on the prognostic model, patients were stratified into either the high-risk or low-risk subgroups with distinct survival. Receiver operating characteristic (ROC) curve analysis was used to identify the predictive accuracy of the model. In relation to different clinical variables, stratification analyses were performed to demonstrate the associations between the expression levels of the six identified NRGs and the clinical variables in CESC. Immunohistochemical (IHC) validation experiments explored abnormal expressions of these six NRGs in CESC. We also explored the relationship between risk score of this necroptosis signature and expression levels of some driver genes in TCGA CESC database and Gene Expression Omnibus (GEO) datasets. Significant relationships between the six prognostic NRGs and immune-cell infiltration, chemokines, tumor mutation burden (TMB), microsatellite instability (MSI), and immune checkpoints in CESC were discovered. In conclusion, we successfully constructed and validated a novel NRG signature for predicting the prognosis of CC patients and might also play a crucial role in the progression and immune microenvironment in CC.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification of a necroptosis-related prognostic gene signature associated with tumor immune microenvironment in cervical carcinoma and experimental verification

Sun

Huang

et al. 2022

World J Surg Onc

View full text Add to dashboard Cite

show abstract

“…Upon stimulation, blood monocytes and tissue macrophages produce IL-1b proprotein, which is cleaved and activated by caspase 1. [10][11][12][13][14][15] According to genome-wide association studies, patients with three common characteristic polymorphisms of this gene, including rs16944, rs1143627 and rs1143634, are highly susceptible to cancer development. [16][17][18] rs1143634, also known as þ3954C>T, is a silent coding sequence polymorphism located in exon 5 of chromosome 2.…”

Section: Introductionmentioning

confidence: 99%

Role of IL-1β rs1143634 (+3954C>T) polymorphism in cancer risk: an updated meta-analysis and trial sequential analysis

2021

Self Cite

View full text Add to dashboard Cite

Objective Oxidative stress caused by the pro-inflammatory cytokine interleukin (IL)-1β has been widely investigated for cancer risk. In this study, we focused on the role of IL-1β rs1143634 polymorphism to reveal its impact on cancer development. Methods Related studies with fixed inclusion criteria were selected from electronic databases to May 2021. This meta-analysis was performed with odds ratios and 95% confidence intervals. Heterogeneity, publication bias and sensitivity analyses were also conducted. Trial sequential analysis (TSA) and in- silico gene expression analysis were performed. Results Forty-four case–control studies involving 18,645 patients with cancer and 22,882 controls were included. We observed a significant association of this single nucleotide polymorphism with overall cancer risk in the codominant model 3 (1.13-fold), recessive model (1.14-fold) and allelic model (1.08-fold). Subgroup analysis revealed that rs1143634 elevated the risk of gastric cancer, breast cancer and multiple myeloma. In addition, Asian and mixed populations and hospital-based controls had a significantly higher risk of cancer development. TSA confirmed our findings. Conclusion Our meta-analysis revealed that the presence of IL-1β rs1143634 polymorphism increases the risk of cancer development. Among polymorphism carriers, the Asian population has a higher risk than other ethnic populations. This meta-analysis was registered retrospectively at INPLASY ( https://inplasy.com/ , INPLASY2021100044).

show abstract

“…Moreover, the selected polymorphisms have a confirmed impact on the course of immune response, the disturbances of which also are observed in the development of BC [ 20 , 21 , 22 ]. Analysis of the available literature also confirmed the influence of the selected polymorphisms on the incidence of numerous cancers, including prostate cancer, breast cancer, cervical cancer, rectal cancer, colon cancer, and gallbladder cancer [ 54 , 55 , 56 , 57 , 58 , 59 ].…”

Section: Methodsmentioning

confidence: 76%

Variability, Expression, and Methylation of IL-6 and IL-8 Genes in Bladder Cancer Pathophysiology

Grębowski

Saluk‐Bijak

Bijak

et al. 2023

IJMS

View full text Add to dashboard Cite

Bladder cancer (BC) is the 10th most common form of cancer globally, but its complete aetiology is still unknown. Nevertheless, there is evidence that chronic inflammation plays a role in the development and progression of BC. Therefore, the presented study aimed to detect a potential association between selected single nucleotide polymorphisms (SNPs)—rs1800797 and rs2069845 in IL-6 and rs2227307 in IL-8—and BC development, as well as to identify the impact of BC on the level of expression and methylation of IL-6 and IL-8 promoters in PBMCs with the use of the TaqMan SNP genotyping assay, TaqMan gene expression assay, and methylation-sensitive high-resolution melting techniques. We did not find any association between the genotypes and combined genotypes of all studied polymorphisms and the occurrence of BC. However, we found that BC patients were characterised by decreased IL-6 and IL-8 mRNA expression levels compared to the controls. Additionally, the methylation status of the IL-6 promoter was higher in controls than in BC patients. Our findings suggest that inflammation may be involved in the development and progression of BC.

show abstract

Detection of association of IL1β, IL4R, and IL6 gene polymorphisms with cervical cancer in the Bangladeshi women by tetra-primer ARMS-PCR method

Cited by 16 publications

References 38 publications

Identification of a necroptosis-related prognostic gene signature associated with tumor immune microenvironment in cervical carcinoma and experimental verification

Identification of a necroptosis-related prognostic gene signature associated with tumor immune microenvironment in cervical carcinoma and experimental verification

Role of IL-1β rs1143634 (+3954C>T) polymorphism in cancer risk: an updated meta-analysis and trial sequential analysis

Variability, Expression, and Methylation of IL-6 and IL-8 Genes in Bladder Cancer Pathophysiology

Contact Info

Product

Resources

About