Detection of Apoptotic Lymphocytes Through Sysmex XN‐1000 As a Diagnostic Marker for Mononucleosis Syndrome

Sale, Silvia; Carone, Addolorata Emanuela; Fumi, Maurizio; Pancione, Ylenia; Rocco, Vincenzo

doi:10.1002/jcla.21938

Cited by 8 publications

(10 citation statements)

References 18 publications

(26 reference statements)

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“…In a recent paper, Sale observed that in patients with MNS, the SSC‐SFL dot plot supplied by Sysmex XN‐10 included a population of presumed apoptotic lymphocytes. Using a particular gating strategy, they counted the percentage of events of the population of apoptotic lymphocytes (Ly‐Apopt).…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…The haematology analysers can measure some positional parameters of the different cell populations in PB that can be useful in the identification of some pathologies, such as granulocytic dysplasia or sepsis. [1][2][3][4] Furthermore, some papers suggest that lymphocyte positional parameters can be useful to differentiate viral diseases (VD) from lymphoproliferative disorders, [5][6][7][8][9] as well as to detect the presence of Sézary cells 10 or mantle cell lymphoma cells. 11 The aim of our study was to investigate the usefulness of the lymphocyte positional parameters in the Sysmex XN haematology analyser to detect changes in the lymphocytes and differentiate among the various lymphoproliferative disorders, mononucleosis syndrome (MNS) and other non-neoplastic lymphocytoses.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Usefulness of the lymphocyte positional parameters in the Sysmex XN haematology analyser in lymphoproliferative disorders and mononucleosis syndrome

Furundarena

Uranga

Sainz

et al. 2017

Int J Lab Hematology

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Usefulness of the lymphocyte positional parameters in the Sysmex XN haematology analyser in lymphoproliferative disorders and mononucleosis syndrome

Furundarena

Uranga

Sainz

et al. 2017

Int J Lab Hematology

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“…Both cell types were lost mostly by DGCun, possibly due to mechanical and chemical destruction. Differences of cell proportions after DGC compared to the respective smear could result from cell apoptosis during DGC since—unlike in a common hemocytometer analysis by light scatter and peroxidase activity—apoptotic cells can easily be excluded during differential smear analysis [29]. RBCL effects on cell concentrations, however, are more difficult to explain.…”

Section: Discussionmentioning

confidence: 99%

Circulating adult stem and progenitor cell numbers—can results be trusted?

et al. 2019

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Background Within the last years, the interest in physical exercise as non-invasive stimulus influencing circulating hematopoietic stem and progenitor cell (CPC) concentrations has constantly grown. Cell estimates are often derived by determining the subgroup of CPC as percent lymphocytes (LYM) or mononuclear cells (MNC) via flow cytometry and back calculation over whole blood (WB) cell counts. However, results might depend on the used cell isolation technique and/or gating strategy. We aimed to investigate MNC loss and apoptosis during the flow cytometry sample preparation process preceded by either density gradient centrifugation (DGC) or red blood cell lysis (RBCL) and the potential difference between results derived from back calculation at different stages of cell isolation and from WB. Methods Human blood was subjected to DGC and RBCL. Samples were stained for flow cytometry analysis of CPC (CD34+/CD45dim) and apoptosis analysis (Annexin V) of MNC and CPC subsets. MNC and LYM gating strategies were compared. Results Both DGC as well as RBCL yielded comparable CPC concentrations independent of the gating strategy when back calculated over WB values. However, cell loss and apoptosis differed between techniques, where after DGC LYM, and monocyte (MONO) concentrations significantly decreased (p < 0.01 and p < 0.05, respectively), while after RBCL LYM concentrations significantly decreased (p < 0.05) and MONO concentrations increased (p < 0.001). LYM apoptosis was comparable between techniques, but MONO apoptosis was higher after DGC than RBCL (p < 0.001). Conclusions Investigated MNC counts (LYM/MONO ratio) after cell isolation and staining did not always mimic WB conditions. Thus, final CPC results should be corrected accordingly, especially when reporting live CPC concentrations after DGC; otherwise, the CPC regenerative potential in circulation could be biased. This is of high importance in the context of non-invasively induced CPC mobilization such as by acute physical exercise, since these cell changes are small and conclusions drawn from published results might affect further applications of physical exercise as non-invasive therapy.

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“…The reference for the Sysmex XN‐Series is the XN‐1000 as a high throughput automated hematology analyzer and was first evaluated in 2012 for both whole blood mode and body fluid mode 4‐6 . Thereafter, its accuracy and precision have been described for various sample types and medical conditions and compared with other leading hematology analyzers 6‐10 . A subset of the XN technology and measurement channels are also used in XN‐L series: A complete blood count (CBC) with a 6‐part white blood cell (WBC) differential, where immature granulocytes (IG) are counted in addition to the 5‐part differential.…”

Section: Introductionmentioning

confidence: 99%

The Sysmex XN‐L (XN‐350) hematology analyzer offers a compact solution for laboratories in niche diagnostics

Khartabil

Frankrijker

Rijke

et al. 2020

Int J Lab Hematology

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Introduction In 2015, Sysmex launched a new series of hematology analyzers (XN‐L Series) designed to fulfill the needs of niche laboratories in areas such as pediatrics, dialysis, neurology, and oncology while providing a compact solution. In this study, we evaluate the whole blood and body fluid modes of one of these analyzers, the XN‐350. Methods A total of 300 residual EDTA samples were measured on the XN‐350 in whole blood mode and the XN‐1000 to evaluate method comparison, flagging sensitivity, repeatability, reproducibility, linearity, carryover, and stability. In addition, 191 samples were obtained and processed in body fluid mode which included, cerebrospinal fluid (CSF), continuous ambulatory peritoneal dialysis (CAPD), ascites, synovial, and pleural fluid to perform method comparison, repeatability, reproducibility, linearity, limit of quantitation, and carryover studies. Results Strong agreement was shown between the XN‐350 and XN‐1000 for both whole blood and body fluid modes in results and flagging. Linearity results in both modes on the XN‐350 showed a high R2 value (>.99). For WBC, RBC, HGB, and PLT, the carryover results were well within the predetermined criteria of ≤0.5% for whole blood and ≤0.3% for CSF. Repeatability and reproducibility were acceptable for both modes, and there were no significant deviations present in stability for whole blood. In addition, there was high agreement in all body fluid types evaluated. Conclusion The performance of the XN‐350 is comparable to the XN‐1000 in both whole blood and body fluid modes, making it a reliable alternative to larger analyzers for smaller, niche laboratories.

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Detection of Apoptotic Lymphocytes Through Sysmex XN‐1000 As a Diagnostic Marker for Mononucleosis Syndrome

Abstract: On Sysmex XN-1000, dot-plot observation allowed immediate detection of IM. Meanwhile, an algorithm based on the parameters on these plots can be calculated with excellent performance.

Cited by 8 publications

References 18 publications

Usefulness of the lymphocyte positional parameters in the Sysmex XN haematology analyser in lymphoproliferative disorders and mononucleosis syndrome

Usefulness of the lymphocyte positional parameters in the Sysmex XN haematology analyser in lymphoproliferative disorders and mononucleosis syndrome

Circulating adult stem and progenitor cell numbers—can results be trusted?

The Sysmex XN‐L (XN‐350) hematology analyzer offers a compact solution for laboratories in niche diagnostics

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