Detection of Apoptosis Induced by Topoisomerase Inhibitors and Serum Deprivation in Syrian Hamster Embryo Cells

Inhibition of lung carcinoma cell growth by high density lipoprotein-associated ?-tocopheryl-succinate

“…However, no PARP cleavage product could be detected in A549 cells that were treated with either free or HDL 3 -associated aTS. Similar observations were reported for Syrian hamster embryo cells that underwent apoptosis (characterized by pronounced DNA fragmentation) in response to topoisomerase inhibitor treatment or serum deprivation [47]. Results obtained during the present study appear to be specific for aTS, as the 86-kDa PARP cleavage product is formed in A549 cells in response to MAL-induced apoptosis [48].…”

Heregulin-induced apoptosis is mediated by down-regulation of Bcl-2 and activation of caspase-7 and is potentiated by impairment of protein kinase C α activity

“…Interestingly, PARP degradation with no appearance of its 85-kDa fragment is found in apoptotic Syrian hamster embryo (SHE) cells (Alexandre et al, 2000a). No induction of caspase-3 activity was noted in apoptotic SHE cells (Alexandre et al, 2000b). These results along with the data presented in Figures 3 and 4d suggest that although PARP is the common substrate of some caspases, proteases other than caspase-3 (such as caspase-7) may degrade PARP without its 85-kDa fragment that is typically seen in caspase-3-mediated PARP cleavage.…”

Caspase-9 plays a marginal role in serum starvation-induced apoptosis

“…Cell culture systems sensitive for serum withdrawal can be subdivided in two groups by their rate of apoptotic response. A rapid time course of apoptosis within a few hours is described for undifferentiated PC-12 cells [1], AKR-2B mouse fibroblasts [2], Syrian hamster embryo cells [3], and myocardiacs [4], for example. A slow time course, which may take several days, was described for NGF-differentiated PC-12 cells [1], HUVEC [5] and myc-overexpressing Rat-1 cells [6], for example.…”