Detection of antiphospholipid antibodies by ELISA-method with bovine thromboplastin as antigen

López‐Soto, Alfons; Casals, F J; Font, Josep; Cervera, Ricard; Ingelmo, M

doi:10.1016/0049-3848(89)90152-7

Cited by 11 publications

(4 citation statements)

References 10 publications

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“…We found no statistically significant difference in the prevalence of the different APA tested, and a good correlation between the ELISA assays. Our study does not confirm a higher prevalence of ATA in LA positive patients previously reported (33). However, an unusually low prevalence of ACA was observed in that study.…”

Section: Discussioncontrasting

confidence: 99%

Clinical Significance of Various ELISA Assays for Detecting Antiphospholipid Antibodies

et al. 1990

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SummaryWe have compared the prevalence of antiphospholipid antibodies (APA) measured by enzyme-linked immunosorbent assay (ELISA), in 119 selected patients using five different antigens: bovine cardiolipin, phosphatidylserine, phosphatidylinositol, bovine partial thromboplastin and human brain partial thromboplastin. All the plasmas have been evaluated for the presence of lupus anticoagulant (LA) activity by clotting techniques. We found a significant association between the incidence of LA and APA (p <0.001), only moderate agreement between the prolongation of the activated partial thromboplastin time (APTT) and ELISAs (r around 0.50) and a good agreement between the ELISAs (r around 0.80). The combination of antibodies against cardiolipin (ACA) and human brain partial thromboplastin (AHPTA) allowed the detection of antibodies in most of the LA positive cases. ACA, AHPTA and antiphosphatidylinositol antibodies detected all the positive samples. Six patients (5%) had a single APA detected. The clinical associations of APA according to phospholipid specificity, immunoglobulin isotype and titer are shown.

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Section: Discussioncontrasting

confidence: 99%

Clinical Significance of Various ELISA Assays for Detecting Antiphospholipid Antibodies

et al. 1990

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“…Patients with fetal loss but no underlying disorder have been found to have lupus anticoagulant in 3 4 8 % of cases and anticardiolipin antibodies in 3-50% of cases (12,13). In a recent study, 59.1% of 352 women with RSA were found to have either IgG or IgM antibody to one of the six phos-Louis, U.S.A.) was used as antigen according to antiphospholipid antibody detection methods previously described (24,25). Briefly, microtitre plates (Nunc, Wiesbaden, Germany) were covered with thromboplastin at a concentration of 50 pg/ml and stored overnight at 4°C.…”

mentioning

confidence: 99%

Recurrent first trimester spontaneous abortion associated with antiphospholipid antibodies:

Marzusch

Dietl

Klein

et al. 1996

Acta Obstet Gynecol Scand

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“…Patients' sera were tested for APA using bovine thromboplastin as antigen according to the methods described by Branch et al [11] and López-Soto et al [56]. The basic enzyme-linked immunosorbent assay (ELISA) method has been recently published [48].…”

Section: Laboratory Methodsmentioning

confidence: 99%

Ocular symptoms in association with antiphospholipid antibodies

Leo-Kottler¹,

Klein

Berg

et al. 1998

Graefe's Archive for Clinical and Experimental Ophthalmology

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Detection of antiphospholipid antibodies by ELISA-method with bovine thromboplastin as antigen

Cited by 11 publications

References 10 publications

Clinical Significance of Various ELISA Assays for Detecting Antiphospholipid Antibodies

Clinical Significance of Various ELISA Assays for Detecting Antiphospholipid Antibodies

Recurrent first trimester spontaneous abortion associated with antiphospholipid antibodies:

Ocular symptoms in association with antiphospholipid antibodies

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