Detection of antigens of the mouse mammary tumor (MTV) and murine leukemia virus (MuLV) in cells of cultures derived from mammary tumors of mice of several strains

Hilgers, J.; Williams, William V.; Myers, B; Dmochowski, L

doi:10.1016/0042-6822(71)90347-3

Cited by 44 publications

(11 citation statements)

References 20 publications

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“…We have chosen the mouse mammary tumor as the experimental model to explore the feasibility of using viral-related proteins in the body fluids as quantitative signals for the presence of solid tumors. Viral antigens have been demonstrated in mammary tumor cell cultures, mammary tumor tissues, and in mouse milk (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11). Furthermore, the level of viral antigens in the milk correlates with the tumor incidence in the mouse strains examined (5,11).…”

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confidence: 99%

Plasma levels of a viral protein as a diagnostic signal for the presence of tumor : the murine mammary tumor model.

Ritzi¹,

Martin²,

Stolfi³

et al. 1976

Proc. Natl. Acad. Sci. U.S.A.

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We used the mouse mammary tumor and its associated virus (murine mammary tumor virus) to examine the possibility of using'plasdia levels of a viral protein (gp52, the glycoprotein of 52,000 molecular weight) as a diagnostic indicator of the presence of a solid tumor. The following features have emerged from our studies: (a) tumor-bearing animals show markedly elevated (100-1000 ng/ml) plasma levels of gp52 and the mean concentration increases with tumor size; (b) mammary tumor tissues located outside the mammary gland are also detected by the elevated plasma gp52; (c) low (2-10 ng/ml)plasma levels of gp52 are found in tumor-free mice, whether they are derived from strains characterized by high or low frequencies of spontaneous mammary tumors; (d) tumor-free lactating females exhibit the normally low levels of plasma gp52 despite the fact that their milk contains an average of 20,000 ng/ml of this antigen' (e) thus, high levels of plasma gp52 are found only in the presence of tumor and are not induced by either predisposition for the disease or by normal production of 'the antigen during lactation; (f) the circulatory clearance time of gp52 is sufficiently rapid to require continued replenishment to maintain the high levels observed in tumor-bearing animals, a feature implying that the gp52 concentration can be a responsive parameter of disease status.The information obtained suggests that plasma gp52 is a potentially useful and specific systemic indicator of the presence and extent of murine mammary neoplasia. We have chosen the mouse mammary tumor as the experimental model to explore the feasibility of using viral-related proteins in the body fluids as quantitative signals for the presence of solid tumors. Viral antigens have been demonstrated in mammary tumor cell cultures, mammary tumor tissues, and in mouse milk (1-11). Furthermore, the level of viral antigens in the milk correlates with the tumor incidence in the mouse strains examined (5, 11). In similar studies comparing individual mice, the concentrations determined by either immunodiffusion (6) or radioimmune assay (9) indicate that mice with higher levels of the antigens in the -milk have a greater tendency to develop tumors. While these studies of milk antigens have been informative, they are not applicable to nonlactating individuals, and it is not evident that the data provide diagnostic information on tumor status.A more generally useful pathway would look to blood plasma as a source of the antigen and to focus on individual viral proteins rather than on whole particles. To this end we devised (12) a convenient method for the purification in high yield of the two gs antigens (gp52, a viral glycoprotein with a 52,000 molecular weight, and p27) of the murine mammary tumor virus (MuMTV). The availability of the pure proteins made it possible for us to develop (12) radioimmune assays sufficiently sensitive to detect these proteins at a level of 1 ng/ml.

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confidence: 99%

Plasma levels of a viral protein as a diagnostic signal for the presence of tumor : the murine mammary tumor model.

Ritzi¹,

Martin²,

Stolfi³

et al. 1976

Proc. Natl. Acad. Sci. U.S.A.

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“…".I2 Further, immunologic studies recently carried out in this laboratory b y rising anti-MTV and anti-MiiLV sera i n MHA and immunofluoresrence tests (IF) on fixed cells of mouse mammary cancer have demonstrated the presence of MTV and MuLV antigens in the same cells. 23 In a study of sera from patients with breast carcinoma, a number of the sera gave cytoplasmic and periiliiclear fluorescence with tissue culture cells derived from tumors of the same or other patients with breast This reaction could not be correlated with the presence of type R or type C virus-like parti-so. (i 'I'rssur CULTURE STUDIES -Seman et al 1441 cles in the t.umor cells.…”

Section: Discussionmentioning

confidence: 99%

Studies on the presence of particles resembling RNA virus particles in human breast tumors, pleural effusions, their tissue cultures, and milk

Seman

Gallager

Lukeman

et al. 1971

Cancer

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Ultrastructural studies have been carried out on biopsies of 84 breast cancers, 13 metastatic lymph nodes, 3 fibroadenomas, and on cells from 33 pleural effusions from breast cancer patients. Breast tumor tissues of 157 patients and pleural effusion cells of 36 patients have been put in tissue culture. Growth has been obtained of cells derived from 47% of the breast tumor biopsies and from 90% of the pleural effusions. However, no cell line could be established. Milk specimens of 4 women with breast cancer and of 17 apparently healthy women also have been examined for the presence of virus particles. Particles resembling murine type B and/or type C virus particles and small virus‐like particles have been observed in a number of specimens. Particles resembling type B and/or type C virus particles have been found in 34 of the 100 breast tumor, metastatic lymph node, and fibroadenoma biopsies. They also have been observed in 2 of 33 specimens of pleural effusions, in 4 of 72 tissue culture specimens, and in 4 of 21 milk specimens. Small virus‐like particles have been observed in 29 of the 100 biopsy specimens and in 3 of 38 tissue cultures derived from breast tumors. They also have been shown in 4 of 21 milk specimens. The relationship of these virus‐like particles to the origin of human breast neoplasia remains to be clarified.

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“…The indirect immuno-fluorescence staining was performed on microscope slides (76 × 26 ram) with swinepox virns-infected pig kidney eell cultures according to the technique described by HILGERS et al (6).…”

Section: Indirect Immuno-2~luoreseence Testmentioning

confidence: 99%

Swinepox. Virus isolation, experimental infections and the differentiation from vaccinia virus infections

Boer

1975

Archives of Virology

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The isolation of swinepox virus in primary pig kidney cell cultures is reported. The differentiation from vaccinia virus was possible with challenge infections of convalescent pigs and the use of the agar gel diffusion precipitation (AGDP) test and immuno-electroosmophoresis (IEOP). Using both immune precipitation tests reactions of identity were obtained between the heterologous antigens of swinepox and vaccinia viruses. A total of 829 pig sera from the field were tested for precipitating antibodies with the IEOP. Antibodies were detected in 65 (=7.8 per cent) of these serum samples.

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Detection of antigens of the mouse mammary tumor (MTV) and murine leukemia virus (MuLV) in cells of cultures derived from mammary tumors of mice of several strains

Cited by 44 publications

References 20 publications

Plasma levels of a viral protein as a diagnostic signal for the presence of tumor : the murine mammary tumor model.

Plasma levels of a viral protein as a diagnostic signal for the presence of tumor : the murine mammary tumor model.

Studies on the presence of particles resembling RNA virus particles in human breast tumors, pleural effusions, their tissue cultures, and milk

Swinepox. Virus isolation, experimental infections and the differentiation from vaccinia virus infections

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