Detection of antibodies to tetanus toxoid: comparison of a direct haemagglutination method with a radioimmunoassay.

Abstract

(vol/ vol) in normal saline and coupled to tetanus toxoid with chromic chloride as described previously.5 6 Briefly, to 0-2 ml of the 50% erythrocytes was added 0 1 ml of 250 LFU/ml toxoid diluted in normal saline and this mixture was mixed on a vortex mixer. Whilst still on the vortex mixer, 0-5 ml of "aged" chromic chloride5 at 0-1 mg/ml was added dropwise. The coupled cells were then held at room temperature 1138 on 10 May 2018 by guest. Protected by copyright.

An Improved Assay for Human Tetanus Anti-Toxin and Its Use in the Accession of Human Plasma for the Production of High-Titre Tetanus Immunoglobulin

“…A number of different approaches have been employed to assay tetanus anti-toxin (TAT), namely, the mouse pro tection bioassay [4][5][6], passive haemagglutination [7,8], double immunodiffusion [9], counter-current immunoelectrophoresis [10], enzyme-linked immunosorbent assay [11], and radioimmunoassay [12,13]. Most of these methods have been developed with short-term research applications in mind and not for large-scale donor screening.…”