Detection of antibodies to Mycoplasma gallisepticum in egg yolk versus serum samples

Brown, Mary Bomberger; Stoll, Margaret L.; Scasserra, Andrea E.; Butcher, Gary D.

doi:10.1128/jcm.29.12.2901-2903.1991

Cited by 15 publications

(3 citation statements)

References 12 publications

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“…When we looked at previous embryonated egg studies, egg yolks were frequently investigated for MG; for example, in general, egg yolk antibodies were examined and compared to those in serum samples by ELISA (Hagan et al, 2004;Brown et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

Comparison of Mycoplasma gallisepticum Infection in Different Samples and Ages of Chicken Breeder Flocks

Demirbilek

Ardicli

Çarlı

2020

Braz. J. Poult. Sci.

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This study aimed to compare method-based and newly developed sample-based methods for Mycoplasma gallisepticum (MG) detection in different samples of breeder flocks suffering from respiratory disease problems by using culture, real-time PCR (rPCR) and ELISA from chicks and embryonated eggs. Overall, 450 samples of 19-day-old chicken embryo's trachea, 450 samples of 8-day-old chicken tracheal swabs and 900 blood samples of 20-, 27-, 34-, 40-and 46-week-old breeder chickens from 5 flocks were sampled for 26 weeks, and were all tested for MG by culture, MG-rPCR and MG-ELISA. Culturing assays and rPCR were applied to 450 mixture samples from 19-day-old chicken embryo's trachea and 450 tracheal swab samples (each pooled into groups of 3) from 8-day-old chicks from the same flocks. Also, 900 blood samples from the same 5 breeder flocks suffering from respiratory disease problems were tested by MG-ELISA. In individual sample-based analyses, 55 (18.3%) of the 300 pooled swab samples were positive for MG using culture methods, and 106 (35.3%) of the same samples were found positive by rPCR (sensitivity, specificity). The ELISAs indicated that 252 (28%) of the 900 breeding blood samples were MG seropositive. Using age-based analyses, the most positive period was 46 weeks, followed by 40 weeks, 34 weeks, 27 weeks and at least 20 weeks, in order of decreasing seropositivity. When comparing the culture and rPCR results of the two different sampling methods, chicken embryo's trachea yielded more positive results than did tracheal swabs from the same flocks. In conclusion, rPCR is a highly specific, sensitive and reliable method for MG identification.

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Section: Discussionmentioning

confidence: 99%

Comparison of Mycoplasma gallisepticum Infection in Different Samples and Ages of Chicken Breeder Flocks

Demirbilek

Ardicli

Çarlı

2020

Braz. J. Poult. Sci.

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“…Samples were read on a commercial microplate reader (BioTek MQX200, BioTek Instruments Inc., Winooski, VT) at an absorbance of 450 nm. Absorbance values for the positive control, negative control, and experimental samples were used to calculate the sample to positive control ratios ( S/P ratios ) ( Brown et al, 1991 ; Davies et al, 2003 ).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Clostridium septicum hemolytic activity, administration route, and dosage volume of a clostridial dermatitis (cellulitis) bacterin-toxoid on humoral immune response in commercial turkeys

Forga

Robbins²,

Smith³

et al. 2023

Poultry Science

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“…d The absorbance obtained for the positive control, negative control, and experimental samples was used to calculate the sample to positive control ratios (S/P ratios). 2,4 An experimental bacterin was produced from the CS isolate that was capable of causing lesions consistent with turkey cellulitis and was recovered from induced lesions, as described. The bacterin was produced from an 18-hr CMM culture of CS, inactivated by the addition of formaldehyde to achieve a final concentration of 0.25%.…”

mentioning

confidence: 99%

Evidence for Clostridium Septicum as a Primary Cause of Cellulitis in Commercial Turkeys

et al. 2009

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Abstract. Etiology and methods of immunoprophylaxis against common field cellulitis in commercial turkeys were evaluated. It was determined that intravenous administration (,10 8 cells/ml) of 1 of 4 isolates of Clostridium septicum from cellulitis lesions rapidly caused the classic lesions of cellulitis followed by death within 36 hr at high doses. When the supernatant alone was injected into turkey poults, signs of depression and ataxia were temporarily observed for up to 20 hr after injection, but no cellulitis lesions were detected. An enzyme-linked immunosorbent assay was developed to measure antibody levels against the isolated C. septicum. This assay was used to predict susceptibility to infection. An experimental formalin-killed bacterin/ toxoid was produced from the challenge strain of C. septicum with inactivation timed to allow toxin accumulation and ,10 8 cells/ml. This bacterin/toxoid given at day of hatch generated a rapid and persistent antibody response against the homologous C. septicum in the vaccinated birds at 9 weeks (P , 0.001). The ability of this experimental vaccine to protect birds in the field as well as its ability to evaluate unvaccinated flocks to establish the time of seroconversion and the relationship to clinical disease are currently under evaluation.

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Detection of antibodies to Mycoplasma gallisepticum in egg yolk versus serum samples

Cited by 15 publications

References 12 publications

Comparison of Mycoplasma gallisepticum Infection in Different Samples and Ages of Chicken Breeder Flocks

Comparison of Mycoplasma gallisepticum Infection in Different Samples and Ages of Chicken Breeder Flocks

Evaluation of Clostridium septicum hemolytic activity, administration route, and dosage volume of a clostridial dermatitis (cellulitis) bacterin-toxoid on humoral immune response in commercial turkeys

Evidence for Clostridium Septicum as a Primary Cause of Cellulitis in Commercial Turkeys

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