Detection of antibodies to Leishmania donovani in animals in a kala-azar endemic region in eastern Sudan: a preliminary report

Mukhtar, Moawia M.; Sharief, Abdalla Hassan; Saffi, S.H. El; Harith, Abdallah el; Higazzi, T.B.; Adam, Andi Muhammad; Abdalla, H S

doi:10.1016/s0035-9203(00)90429-2

Cited by 50 publications

(61 citation statements)

References 12 publications

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“…The literature is scarce regarding serological diagnosis of leishmaniosis in rats; we could only find two studies using an immunochromatographic method (LIMA et al, 2013;SINGH et al, 2013), two using ELISA (MUKHTAR et al, 2000;BARBOSA, 2005) in Sudan and Brazil, and two using IFAT (BEN-ISMAIL et al, 1989;CÁSSIA-PIRES et al, 2014) in Tunisia and Brazil. Our prevalence based on ELISA was higher (23.3%) than previously reported (0% in Brazil and 4.1% in Sudan) and based on IFAT was lower (3.3%) than that of previous reports (27.2% in Tunisia and 51.3% in Brazil).…”

Section: Discussionmentioning

confidence: 94%

Leishmania in synanthropic rodents (Rattus rattus): new evidence for the urbanization of Leishmania (Leishmania) amazonensis

Caldart

Freire

Ferreira

et al. 2017

Rev. Bras. Parasitol. Vet.

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Leishmania in synanthropic rodents (Rattus rattus):new evidence for the urbanization of Leishmania (Leishmania) amazonensis AbstractThis study aimed to detect parasites from Leishmania genus, to determine the prevalence of anti-Leishmania spp. antibodies, to identify circulating species of the parasite, and to determine epidemiological variables associated with infection in rats caught in urban area of Londrina, Paraná, Brazil. Animal capture was carried out from May to December 2006, serological and molecular methods were performed. DNA was extracted from total blood, and nested-PCR, targeting SSu rRNA from Leishmania genus, was performed in triplicate. The positive samples were sequenced twice by Sanger method to species determination. In total, 181 rodents were captured, all were identified as Rattus rattus and none showed clinical alterations. Forty-one of the 176 (23.3%) animals were positive for Leishmania by ELISA and 6/181 (3.3%) were positive by IFAT. Nine of 127 tested animals (7.1%) were positive by PCR; seven were identified as L.

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Section: Discussionmentioning

confidence: 94%

Leishmania in synanthropic rodents (Rattus rattus): new evidence for the urbanization of Leishmania (Leishmania) amazonensis

Caldart

Freire

Ferreira

et al. 2017

Rev. Bras. Parasitol. Vet.

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“…Serological evidences of anti- L . donovani antibodies in different domestic animals including cattle were reported in Sudan [14]. In a recent study in Nepal, Leishmania DNA was detected in several domestic animals including cattle from an endemic area [15].…”

Section: Introductionmentioning

confidence: 99%

Survey of domestic cattle for anti-Leishmania antibodies and Leishmania DNA in a visceral leishmaniasis endemic area of Bangladesh

et al. 2011

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BackgroundVisceral leishmaniasis (VL), caused by an intracellular parasite Leishmania donovani in the Indian subcontinent, is considered to be anthroponotic. The role of domestic animals in its transmission is still unclear. Although cattle are the preferred blood host for Phlebotomus argentipes, the sandfly vector of VL in the Indian subcontinent, very little information is available for their role in the disease transmission. In this study, we examined domestic cattle for serological and molecular evidence of Leishmania infection in a VL-endemic area in Bangladesh. Blood samples from 138 domestic cattle were collected from houses with active or recently-treated VL and post-kala-azar dermal leishmaniasis patients. The presence of anti-leishmanial antibodies in serum was investigated using enzyme-linked immunosorbent assay (ELISA) and then with direct agglutination tests (DAT). Nested PCR (Ln PCR) was performed to amplify the ssu-rRNA gene using the DNA extracted from Buffy coat. Recently-developed molecular assay loop-mediated isothermal amplification (LAMP) was also performed for further sensitive detection of parasite DNA.ResultsIn this study, 9.4% (n = 13) of the cattle were found to be positive by ELISA. Of the 13 ELISA-positive cattle, only four (30.8%) were positive in DAT. Parasite DNA was not detected in either of the molecular assays (Ln PCR and LAMP).ConclusionsThe study confirmed the presence of antibodies against Leishmania parasite in cattle. However, the absence of Leishmania DNA in the cattle indicates clearly that the cattle do not play a role as reservoir host. Similar study needs to be undertaken in the Indian subcontinent to determine the role of other domestic animals on which sandflies feed.

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“…Studies in Sudan and Kenya have detected L. donovani in domestic animals ( 2 , 3 ), but whether these play a role in Uganda is unknown. In Uganda, the disease appears to be restricted to Pokot County, a semiarid lowland area in Nakapiripirit District (Appendix Figure).…”

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confidence: 99%

Kala-azar Control, Uganda

Kolaczinski

Worku

Chappuis

et al. 2007

Emerg. Infect. Dis.

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Detection of antibodies to Leishmania donovani in animals in a kala-azar endemic region in eastern Sudan: a preliminary report

Cited by 50 publications

References 12 publications

Leishmania in synanthropic rodents (Rattus rattus): new evidence for the urbanization of Leishmania (Leishmania) amazonensis

Leishmania in synanthropic rodents (Rattus rattus): new evidence for the urbanization of Leishmania (Leishmania) amazonensis

Survey of domestic cattle for anti-Leishmania antibodies and Leishmania DNA in a visceral leishmaniasis endemic area of Bangladesh

Kala-azar Control, Uganda

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