Detection of Antibiotics and Evaluation of Antibacterial Activity with Screen-Printed Electrodes

Munteanu, Florentina-Daniela; Titoiu, Ana Maria; Marty, Jean‐Louis; Vasilescu, Alina

doi:10.3390/s18030901

Cited by 76 publications

(34 citation statements)

References 122 publications

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“…Biorecognition is one of the most common strategies and uses aptamer-based sensors [23][24][25][26][27][28][29][30][31][32], which are the most recent and commonly found in the literature, followed by enzyme-linked immunoassay (ELISA) functionalization [14,15,33,34], and sensors based on molecular imprinted polymers (MIPs) [35,36] or antibodies [8,37,38] (see Table S2). Furthermore, the oxidation of TCs has been reported using diverse types of electrodes and materials, ranging from metallic nanoparticles [39][40][41][42][43][44][45], graphene oxide [46][47][48][49][50], composites [43,[51][52][53][54][55], gold [3,11,56], boron-doped diamond electrode (BDDE) [57][58][59][60], ruthenium oxide-hexacyanor-uthenate (RuO-RuCN) [61], or multiwall carbon nanotubes (MWN...…”

Section: Introductionmentioning

confidence: 99%

Tetracycline Antibiotics: Elucidating the Electrochemical Fingerprint and Oxidation Pathway

et al. 2021

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Herein, a complete study of the electrochemical behavior of the most commonly used tetracycline antibiotics (TCs) on unmodified carbon screen-printed electrodes (SPEs) is presented. In addition, the oxidation pathway of TCs on SPE is elucidated, for the first time, with liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-QTOF-MS). Square wave voltammetry (SWV) was used to study the electrochemical fingerprint (EF) of the antibiotics shaping the different oxidation processes of the TCs in a pH range from 2 to 12. Their characteristic structure and subsequent EF offer the possibility of distinguishing this class of antibiotics from other types. Under the optimized parameters, calibration curves of tetracycline (TET), doxycycline (DOXY), oxytetracycline (OXY), and chlortetracycline (CHL) in a Britton Robinson buffer solution (pH 9) exhibited a linear range between 5 and 100 µM with excellent reproducibilities (RSDTET = 3.01%, RSDDOXY = 3.29%, RSDOXY = 9.78% and RSDCHL = 6.88% at 10 µM, N = 3) and limits of detection (LOD) of LODTET = 4.15 µM, LODDOXY = 2.14 µM, LODOXY = 3.07 µM and LODCHL = 4.15 µM. Furthermore, binary, tertiary, and complex mixtures of all TCs were analyzed with SWV to investigate the corresponding EF. A dual pH screening (pH 4 and pH 9), together with the use of a custom-made Matlab script for data treatment, allowed for the successful confirmation of a single presence of TCs in the unknown samples. Overall, this work presents a straightforward study of the electrochemical behavior of TCs in SPE, allowing for the future on-site identification of residues of tetracycline antibiotics in real samples.

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Section: Introductionmentioning

confidence: 99%

Tetracycline Antibiotics: Elucidating the Electrochemical Fingerprint and Oxidation Pathway

et al. 2021

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“…The application of nanomaterials in stack architectures is very promising for the development of high stable SC-ISEs with long operating lifetimes. [118] Several screen-printed electrodes (SPEs) modified with nanocomposites were also developed for electrochemical determinations of e.g., heavy metal and antibiotics in real environmental and food samples [119][120][121][122]. The use of SPEs modified by carbon black was also described for phenyl carbamate pesticides (carbofuran, isoprocarb, carbaryl and fenobucarb) detection in grain samples.…”

Section: Analyte Detection Based On Electrochemical Bio/chemosensorismentioning

confidence: 99%

Nanostructured Organic/Hybrid Materials and Components in Miniaturized Optical and Chemical Sensors

et al. 2020

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In the last decade, biochemical sensors have brought a disruptive breakthrough in analytical chemistry and microbiology due the advent of technologically advanced systems conceived to respond to specific applications. From the design of a multitude of different detection modalities, several classes of sensor have been developed over the years. However, to date they have been hardly used in point-of-care or in-field applications, where cost and portability are of primary concern. In the present review we report on the use of nanostructured organic and hybrid compounds in optoelectronic, electrochemical and plasmonic components as constituting elements of miniaturized and easy-to-integrate biochemical sensors. We show how the targeted design, synthesis and nanostructuring of organic and hybrid materials have enabled enormous progress not only in terms of modulation and optimization of the sensor capabilities and performance when used as active materials, but also in the architecture of the detection schemes when used as structural/packing components. With a particular focus on optoelectronic, chemical and plasmonic components for sensing, we highlight that the new concept of having highly-integrated architectures through a system-engineering approach may enable the full expression of the potential of the sensing systems in real-setting applications in terms of fast-response, high sensitivity and multiplexity at low-cost and ease of portability.

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“…Electrochemical sensors are devices that produce electrical signals, proportional to the analyte concentrations to which they are exposed [6]. These devices have proven to be rapid, precise, selective, sensitive and easy to use analytical tools for the analysis of a wide range of environmental samples including antibiotic residues [7,8]. Norfloxacin has previously been detected, anodically, at carbon electrode surfaces.…”

Section: Introductionmentioning

confidence: 99%

Voltammetric and Impedimetric Detection of Norfloxacin at Co Nanoparticle Modified Polymer Composite Electrodes

2020

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The measurement of antibiotics in environmental water systems is increasingly becoming a top priority for global environmental watchdog organisations, as the looming threat of emerging contaminants moves to centre stage. A novel chemical sensor based on polyamic acid (PAA) semiconducting polymer and cobalt nanoparticles (CoNP) was developed and used to demonstrate proof of concept evidence for measurement of norfloxacin at trace concentrations in aqueous systems. Polyamic acid and cobalt nanoparticles were both chemically synthesised and characterised using Fourier transform infrared spectroscopy, transmission electron microscopy, cyclic voltammetry and small angle x-ray scattering. The polyamic acid and cobalt nanoparticles were electrodeposited onto screen printed carbon electrodes to produce novel composite sensors (SPCE/PAA/CoNP). The polymer composite chemical sensors were applied to the detection of norfloxacin in the micromolar concentration using square wave voltammetry, with a sensitivity of 18.0 � 6.59 μA/mM, calculated from the slope of the calibration curve and a limit of detection (LOD) of 0.979 � 0.419 mM, with LOD = 3.3(Sy/S). The SPCE/PAA/ CoNP sensor response to norfloxacin as measured by electrochemical impedance spectroscopy, yielded a sensitivity of 17.6 � 8.72 Ω/mM as and a limit of detection (LOD) of 0.228 � 0.0935 mM.

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Detection of Antibiotics and Evaluation of Antibacterial Activity with Screen-Printed Electrodes

Cited by 76 publications

References 122 publications

Tetracycline Antibiotics: Elucidating the Electrochemical Fingerprint and Oxidation Pathway

Tetracycline Antibiotics: Elucidating the Electrochemical Fingerprint and Oxidation Pathway

Nanostructured Organic/Hybrid Materials and Components in Miniaturized Optical and Chemical Sensors

Voltammetric and Impedimetric Detection of Norfloxacin at Co Nanoparticle Modified Polymer Composite Electrodes

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