Detection of antibiotic resistance genes in samples from acute and chronic endodontic infections and after treatment

Rôças, Isabela N.; Siqueira, José F.

doi:10.1016/j.archoralbio.2013.03.010

Cited by 49 publications

(33 citation statements)

References 33 publications

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“…The most frequently adopted exclusion criteria were previous exposure to antimicrobial agents, ranging from 1 to 6 months. The presence of resistance genes was assessed in clinical isolates (38-40, 43, 44, 46) and in samples from each environment (41,42,45). The presence of resistance genes in saliva samples was determined only from healthy subjects (38,41).…”

Section: Resultsmentioning

confidence: 99%

Distribution of Genes Related to Antimicrobial Resistance in Different Oral Environments: A Systematic Review

Moraes

Só

Pizzol

et al. 2015

Journal of Endodontics

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Section: Resultsmentioning

confidence: 99%

Distribution of Genes Related to Antimicrobial Resistance in Different Oral Environments: A Systematic Review

Moraes

Só

Pizzol

et al. 2015

Journal of Endodontics

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“…5 Recent studies have investigated the presence of genes exhibiting bacterial resistance to antibiotics, including the cfxA/cfxA2 gene, in clinical samples from endodontic infections. 6,7,8 However, no report has investigated the presence of these genes in oral cavity sites, such as the supragingival plaque, which is an important source of microorganisms for endodontic infections. This study aimed to identify the presence of microorganisms and virulence factors associated with antimicrobial resistance in samples from different niches of the oral cavity in different clinical situations.…”

Section: Introductionmentioning

confidence: 99%

Saliva, supragingival biofilm and root canals can harbor gene associated with resistance to lactamic agents

et al. 2015

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This study aimed to determine the presence of Prevotella strains and genes associated with resistance to lactamics in different oral niches from patients with/without primary endodontic infections. Saliva (S) and supragingival biofilm (SB) were collected from three patient groups: Group I -no endodontic infection (n = 15); Group II -acute endodontic infection (n = 12); and Group III -chronic endodontic infection (n = 15). Root canal (RC) samples were collected from Groups II and III. The presence of P. intermedia, P nigrescens, P. tannerae and cfxA/cfxA2 gene was assessed by PCR. The cfxA/cfxA2 gene was not detected in all environments within the same patient. The cfxA/cfxA2 gene was present in 23.81% of S samples, 28.57% of SB samples, and 7.41% of RC samples. Prevotella species were detected in 53.97%, 47.62% and 34.56% of the S, SB, and RC samples, respectively. P. intermedia had a high frequency in saliva samples from Group 3. Saliva samples from Group 1 had higher detection rates of P. nigrescens than did Groups 2 and 3. Patients without endodontic disease had high frequencies of P. nigrescens in the SB samples. The presence or absence of spontaneous symptoms was not related to the detection rates for resistance genes in the RC samples. Saliva, supragingival biofilm and root canals can harbor resistant bacteria. The presence of symptomatology did not increase the presence of the cfxA/cfxA2 gene in the supragingival biofilm and inside root canals.

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“…Après le diagnostic clinique, le prélèvement a été réalisé selon la méthode de Rôcas et Siqueira [15]. En effet, avant le prélèvement de l'exsudat, chaque patient a rincé la cavité buccale pendant quelques secondes avec une solution de chlorhexidine à 0,12 %.…”

Section: Collecte Des éChantillonsunclassified

Détection d’Acinetobacter baumannii, agent pathogène opportuniste et multirésistant dans les infections bucco-dentaires à Ouagadougou, Burkina Faso

Kaboré¹,

Konaté²,

Bako³

et al. 2016

Med Buccale Chir Buccale

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Résumé -Introduction :Acinetobacter est une bactérie opportuniste multirésistante. Cette étude visait à détecter Acinetobacter baumanii dans les infections bucco-dentaires au Burkina Faso. Cette bactérie a été retenue compte tenu du caractère émergent de sa résistance aux antibiotiques dans le monde, de sa pathogénicité opportuniste, de son pouvoir de diffusion épidémique et de l'impasse thérapeutique qu'elle engendre. Matériel et méthodes : C'est une étude prospective conduite au Centre municipal de santé bucco-dentaire de Ouagadougou de juin 2014 à octobre 2014. Les données cliniques ont été recueillies à l'aide d'une fiche de collecte. Les bactéries ont été identifiées avec la galerie API 20E. La sensibilité aux antibiotiques et la détection de β-lactamases à spectre étendu (BLSE) ont été réalisées par la méthode de diffusion sur milieu gélosé. Résultats : Une prédominance de la tranche d'âge 19-40 ans (55,2 %) sur les 125 patients reçus a été observée. Trois souches d'Acinetobacter baumannii (2,4 %) ont été isolées des cellulites chroniques et identifiées. Elles étaient totorésistantes aux 21 antibiotiques testés et ne produisaient pas de BLSE. Conclusion : Au regard de la totorésistance d'Acinetobacter baumannii et de l'impasse thérapeutique qu'elle engendre, sa détection chez un patient doit faire l'objet de déclaration officielle. Abstract -Introduction:Acinetobacter is an opportunistic and multidrug-resistant (MDR) bacterium, implicated in nosocomial and community infections. This study aimed to detect the MDR species Acinetobacter baumanii involved in oral infections in Burkina Faso. This bacterium was chosen in view of the emerging character of its antibiotic resistance in the world, its opportunistic pathogenicity, its epidemic spread and the therapeutic stalemate generated. Material and Methods: This was a prospective study conducted at the «Centre Municipal de Santé Bucco-dentaire de Ouagadougou» from June to October 2014. Clinical data were collected using a collection sheet. Pathogenic bacteria were identified by API 20E. Antibiotic sensitivity and detection of extended spectrum β-lactamase (ESBL) were performed by the diffusion method on agar medium. Results: Of the 125 patients treated, the age group 19-40 years (55.2%) was predominant. Three strains of Acinetobacter baumannii (2.4%) were isolated and identified in chronic cellulitis. They were toto-resistant to the 21 antibiotics tested and did not produce ESBL. Conclusion: Given the multidrug resistance of Acinetobacter baumannii and the therapeutic stalemate generated, its detection in a patient needs to be declared in an official statement. Mots clés :Acinetobacter baumannii / cellulite / parodontite apicale / totorésistance Med Buccale Chir Buccale 2016;22:105-112

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Detection of antibiotic resistance genes in samples from acute and chronic endodontic infections and after treatment

Cited by 49 publications

References 33 publications

Distribution of Genes Related to Antimicrobial Resistance in Different Oral Environments: A Systematic Review

Distribution of Genes Related to Antimicrobial Resistance in Different Oral Environments: A Systematic Review

Saliva, supragingival biofilm and root canals can harbor gene associated with resistance to lactamic agents

Détection d’Acinetobacter baumannii, agent pathogène opportuniste et multirésistant dans les infections bucco-dentaires à Ouagadougou, Burkina Faso

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