Detection of anti-testicular antibodies among infertile males using indirect immunofluorescent technique

Abstract

Introduction and Aim: The antinuclear antibodies (ANA) are unwanted molecules which bind and destroy certain structures within the nucleus. Immunofluorescence is a powerful technique that utilizes fluorescent-labeled antibodies to detect specific target antigens. The aim of this study was to detect the anti-testicular antibody among infertile males in Baghdad city and determine the most common type of infertility. Materials and Methods: The study involved 73 male (53 infertile and 20 non-infertile) volunteers, at Kamal Al-Samarrai Teaching Hospital in Baghdad, Iraq. Serum collected from the study subjects was tested for steroid-cell antibodies (STC-Ab), anti-nuclear antibody (ANA) and anti-testicular antibodies (ATCA) by Indirect Immunofluorescence assay (IIFA). Data obtained was subjected to statistical analysis using the SPSS program. Results: In the current study 52.9% of infertile men tested positive for testicular antibodies compared to the control group. The highest rate of testicular cell antibodies was observed in the serum of infertile patients aged between 30-39 years and the lowest in patients aged 50-59 years. The marriage duration among those showing the highest percentage of antibodies against testicular cells was 1-10 years. Study for the association of male infertility type to those positive for ATCA showed almost all (85.7%) patients with necrozoospermia to be positive for ATCA. This was followed by males with azoospermia (50%) and oligospermia (46.9%). The patients were negative for Addison’s disease while a few (28.6%) were positive for lupus erythematosus.

Prevalence of Methicillin-Resistant and Methicillin Sensitive Staphylococcus aureus Nasal Carriage and their Antibiotic Resistant Patterns in Kirkuk City, Iraq

“…The polymerase chain reaction (PCR) was applied in the detection of pathogenic microorganisms, [50][51][52][53][54][55][56][57][58][59][60] and genetic-related diseases. [61][62][63][64]…”

The effect of Mentha piperita oil in improving serotonin level, physiological factors, antioxidants and fertility markers in male rabbits on opioid oxycodone withdrawal

“…Results for sperm activity in this study is in agreement with earlier studies by Luo et al, (24) and Soltani et al, (25), who report that the essential oils in M. peperita are a vital substrate for the sperm through the oxidation of fatty acids as a source of energy, which contributes to increasing the effectiveness of the sperm and their maturation as well as in maintaining their concentration, composition and activity (24,25). It can be recommended to apply M. peperita in the treatment of anti-testicular antibodies in wide ratio of infertile males (26). Thus, our study shows a positive role for M. peperita oils, in withdrawal of opioid oxycodone addiction.…”

The protective role of adiponectin in restoring spermatogenesis in mice fed on methionine-choline deficient diet

“…A meta-analysis study found that seminal fluid quality (sperm count and concentration) had fallen by 50-60% worldwide in the previous four decades, raising severe concerns about fertility in the near future (1). Anti-testicular antibodies (ATCA) were most prevalent contributing to infertility (2), but malnutrition, sedentary lifestyles, alcohol consumption, smoking, pollution, underlying health conditions, and stress have also been implicated as major causes of male infertility (3). It has been estimated that a healthy male produces over 200 million sperm cells daily within the testis through the spermatogenesis process that involves the continuous production of spermatozoa cells (4).…”

“…The steroid testosterone, produced by the testis is essential in maintaining and regulating normal spermatogenesis (5). Studies have shown that spermatogenesis is greatly influenced by genetic, nutritional and lifestyle factors which play an essential role in male reproductive health and fertility (2). Research conducted in recent years has shown diet, balanced nutrition to have a significant impact on male fertility (6,7,8).…”