Detection of anti-pituitary autoantibodies by immunoblotting

Crock, Patricia; Salvi, Mario; Miller, A.; Wall, Jack R.; Guyda, H.

doi:10.1016/0022-1759(93)90404-u

Cited by 73 publications

(44 citation statements)

References 14 publications

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“…When serum fT3 and fT4 levels decrease due to decreased TSH secretion, TRH increases and prolactin level may increase. However, hyperprolactinemia is observed only in up to 20% of cases even in hypothyroidism [14]. In our series, serum fT3 and fT4 levels were within the reference ranges, although serum TSH level was low.…”

Section: Discussionsupporting

confidence: 45%

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Longitudinal Study of Patients with Idiopathic Isolated TSH Deficiency: Possible Progression of Pituitary Dysfunction in Lymphocytic Adenohypophysitis

et al. 2006

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Abstract. The relationship between isolated TSH deficiency and hypophysitis was studied. Six patients (five women and one man) with idiopathic isolated TSH deficiency were longitudinally investigated with an interval of 31 to 60 months. Clinical symptoms, laboratory results and endocrine function were investigated as well as pituitary magnetic resonance imaging (MRI) at the start and the end of the study. Clinically, initial symptoms due to hypothyroidism were ameliorated by the thyroid hormone replacement in all patients. Oligomenorrhea newly appeared during the study in three patients, although no other symptoms appeared. Serum fT3 and fT4 levels were within the reference ranges, and serum TSH level and its response to TRH stimulation remained low in all patients. Peak plasma GH level during GRH stimulation was significantly (p<0.03) decreased, at the end of the study as compared with the start. Peak plasma FSH level to LHRH stimulation was significantly (p<0.03) decreased as well as basal FSH level. In contrast, peak of prolactin during TRH stimulation was significantly (p<0.03) increased at the end of the study as compared with the start as well as basal prolactin level. Endocrine features at the end of the study were compatible with those of lymphocytic adenohypophysitis (LAH). MRI of the pituitary gland showed empty sella in one patient and slight swelling in two patients. These findings remained unchanged during the study period. One patient underwent pituitary biopsy, with histological examination showing atypical form of LAH. LAH can cause idiopathic isolated TSH deficiency and can functionally progress to combine dysfunction of the pituitary gland.

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Section: Discussionsupporting

confidence: 45%

“…The methods of measuring plasma or serum hormone levels and antibodies are described elsewhere [1]. APA was measured by the method using Western blotting as described previously by Crock et al [14]. In short, normal human pituitary glands were homogenized in PBS and were centrifuged for the separation of the cytosol and the membrane fractions.…”

Section: Methodsmentioning

confidence: 99%

Longitudinal Study of Patients with Idiopathic Isolated TSH Deficiency: Possible Progression of Pituitary Dysfunction in Lymphocytic Adenohypophysitis

et al. 2006

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“…IB to detect pituitary antibodies was first reported in 1993 by Crock and colleagues [19]. We have recently compared IF and IB and found that IB was more sensitive (64% vs. 57%) and specific (86% vs. 76%) than IF in predicting histologically-proven AH.…”

Section: Pituitary Antibodiesmentioning

confidence: 85%

Pituitary autoimmunity: 30 years later

Caturegli

Lupi

Landek-Salgado

et al. 2008

Autoimmunity Reviews

159

147

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Pituitary autoimmunity encompasses a spectrum of conditions ranging from histologically proven forms of lymphocytic hypophysitis to the presence of pituitary antibodies in apparently healthy subjects. Hypophysitis is a rare but increasingly recognized disorder that typically presents as a mass in the sella turcica. It mimics clinically and radiologically other non-secreting sellar masses, such as the more common pituitary adenoma. Hypophysitis shows a striking temporal association with pregnancy, and it has been recently described during immunotherapies that block CTLA-4. Several candidate pituitary autoantigens have been described in the last decade, although none has proven useful as a diagnostic tool. This review summarizes the advances made in the field since the publication of the first review on pituitary autoimmunity, and the challenges that await clarification.

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“…Serum FT 4 and FT 3 (Vitros Immunodiagnostic Products, Ortho-Clinical Diagnostics Inc., Rochester, NY, USA), TSH (Immulite 2000 third generation TSH, Diagnostic Products Corp., Los Angeles, CA, USA) were assayed by commercial kits in all patients and controls. Normal values are as follows: FT 4 , 7.0-17.0 pg/ml; FT 3 , 2.7-5.7 pg/ml; TSH, 0.4-3.4 mU/ml.…”

Section: Assaysmentioning

confidence: 99%

Serum pituitary antibodies in normal pregnancy and in patients with postpartum thyroiditis: a nested case–control study

Manetti

Parkes²,

Lupi³

et al. 2008

European Journal of Endocrinology

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Objectives: The aim of this study was to evaluate antipituitary antibody (APA) prevalence in a series of patients with postpartum thyroiditis (PPT) during pregnancy and in the postpartum. Design: We conducted a nested case-control study on consecutive PPT and normal pregnant women at the Centre for Endocrine and Diabetes Sciences in Cardiff and at the Department of Endocrinology in Pisa. Methods: We enrolled 30 women with PPT: 17 were hypothyroid (Hypo), 7 with hyperthyroidism (Hyper) and 6 with a transient hyperthyroidism followed by hypothyroidism (Biphasic). Twenty-one healthy pregnant women served as controls. APA (measured using indirect immunofluorescence), free thyroxine, free triiodothyronine, TSH, antithyroid autoantibodies, and thyroid ultrasound were performed during pregnancy and postpartum. The stored sera have been sent to Pisa, where serum APA, IGF1, and cortisol were measured. Results: APA were found in 8 out of the 30 PPT patients (26.7%) and in one normal pregnancy (4.7%, PZ0.063). Three out of the seventeen Hypo with PPT (17.6%), three out of the seven Hyper PPT (42.8%), and two out of the six Biphasic PPT (33.3%) were positive for APA. APA prevalence was not significantly different in the PPT subgroups (PZ0.453). With one exception, APA all increased in the postpartum period (87.5%, P!0.016). Basal serum IGF1 and cortisol were in the normal range with the exception of two patients with positive APA who presented low serum IGF1 levels (36 and 45 ng/ml). Conclusions: APA are frequently present in the postpartum period in patients affected by PPT. Further studies are necessary to evaluate whether APA in PPT patients are associated with pituitary function impairment.European Journal of Endocrinology 159 805-809

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Detection of anti-pituitary autoantibodies by immunoblotting

Cited by 73 publications

References 14 publications

Longitudinal Study of Patients with Idiopathic Isolated TSH Deficiency: Possible Progression of Pituitary Dysfunction in Lymphocytic Adenohypophysitis

Longitudinal Study of Patients with Idiopathic Isolated TSH Deficiency: Possible Progression of Pituitary Dysfunction in Lymphocytic Adenohypophysitis

Pituitary autoimmunity: 30 years later

Serum pituitary antibodies in normal pregnancy and in patients with postpartum thyroiditis: a nested case–control study

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